4‑Hydroxynonenal Antibody Best Seller

Catalog # MAB3249 | R&D Systems, Inc. a Bio-Techne Brand
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MAB3249
MAB3249-SP
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Key Product Details

Species Reactivity

Multi-Species

Applications

Immunohistochemistry, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 198960

Product Summary for 4‑Hydroxynonenal Antibody

Immunogen

KLH-coupled 4-Hydroxynonenal

Specificity

Detects 4-Hydroxynonenal adducts of histidine residues. No cross-reactivity with nitrotyrosine, formaldehyde, glutaraldehyde, or 4‑hydroxy hexenaldehyde adducts is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for 4‑Hydroxynonenal Antibody

Detection of Human 4-Hydroxynonenal antibody by Western Blot.

Detection of Human 4‑Hydroxynonenal by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 4-Hydroxynonenal (4-HNE). PVDF membrane was probed with 1 µg/mL of Mouse Anti-4-Hydroxynonenal Monoclonal Antibody (Catalog # MAB3249) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for 4-Hydroxynonenal at adducts of histidine residues (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human 4-Hydroxynonenal antibody by Simple WesternTM.

Detection of Human 4‑Hydroxynonenal by Simple WesternTM.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 4-Hydroxynonenal (4-HNE), loaded at 0.2 mg/mL. Specific bands were detected for 4‑Hydroxynonenal at adducts of histidine residues kDa (as indicated) using 5 µg/mL of Mouse Anti-4‑Hydroxynonenal Monoclonal Antibody (Catalog # MAB3249). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
4-Hydroxynonenal antibody in Human Prostate by Immunohistochemistry (IHC-P).

4‑Hydroxynonenal in Human Prostate.

4-Hydroxynonenal was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-4-Hydroxynonenal Monoclonal Antibody (Catalog # MAB3249) at 0.1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Applications for 4‑Hydroxynonenal Antibody

Application
Recommended Usage

Immunohistochemistry

0.1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human normal prostate and prostate cancer tissue

Simple Western

5 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line treated with 4-Hydroxynonenal (4-HNE)

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line treated with 4-Hydroxynonenal (4-HNE)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 4.3 using MAB3249 in the following applications:

Published Applications

Read 38 publications using MAB3249 in the following applications:

Formulation, Preparation and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 4-Hydroxynonenal

4-hydroxy-2-nonenal (4-hydroxynonenal, 4-HNE) is a highly reactive aldehyde generated by the exposure of polyunsaturated fatty acids to peroxides and reactive oxygen species (ROS). It non-enzymatically forms stable protein adducts with histidine, lysine, and cysteine side chains that have been used as biomarkers for oxidative damage in cells. Conditions where 4-HNE immunoreactivity has been observed include include inflammation, neurodegenerative diseases, and ischemic damage to the heart and brain.

Alternate Names

4Hydroxynonenal, HNE

Product Documents for 4‑Hydroxynonenal Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for 4‑Hydroxynonenal Antibody

For research use only

Citations for 4‑Hydroxynonenal Antibody (38)

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Customer Reviews for 4‑Hydroxynonenal Antibody (3)

4.3 out of 5
3 customer ratings
5 stars
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3 stars
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  • 4-Hydroxynonenal Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Liver tissue
    Species: Mouse
    Verified Customer | Posted 08/11/2021
    4‑Hydroxynonenal Antibody MAB3249
  • 4-Hydroxynonenal Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Aortic valves
    Species: Mouse
    Verified Customer | Posted 03/29/2021
    -Fixed in 10% neutral buffered formalin followed by dehydration process -10 um thick cryosection - Blocking: Normal Goat Serum (NGS) - Primary Ab (4-HNE): diluted in NGS at 20 ug/mL; 4C overnight - Secondary (AF488): 1:100 dilution in NGS; room temp 1hr - Vector anti-fade mounting media with DAPI
    4‑Hydroxynonenal Antibody MAB3249
  • Name: cynthia morimoto
    Application: Western Blot
    Sample Tested: See PMID 21429624
    Species: Mouse
    Verified Customer | Posted 02/19/2015

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FAQs for 4‑Hydroxynonenal Antibody

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  • Q: For the Western Blot data image for Catalog # MAB3249, how long were the HepG2 cells treated with 4-HNE prior to analysis?

    A: The HepG2 cells were treated with 100 µM 4-HNE for 1 hour before lysing the cells.

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Reconstitution Calculator

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