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Advanced Neuroscience Research and Development with Simple Western

Advanced Neuroscience with the Most Data from Precious Samples

Some of the most prevalent and least understood diseases are those that affect the nervous system. Simple Western™ is winning the race to discovery in neuroscience research and development with high sensitivity multiplex protein assays at unprecedented speeds. Get the most data out of your precious sample. With Single-Cell Western on Milo™, you can measure heterogeneity in your samples and study protein expression within specific rare neural cell subtypes.

Advanced neuroscience research with Simple Western requires only 3 µL of sample while offering pg-level sensitivity, so there is no longer any need to pool samples like mouse tissue. Get single-animal statistical data and realize significant cost savings and save mouse lives. Simple Western also ups the data point count on laser capture microdissection (LCM) samples.

Mouse Tissue Western Blot Data with Simple Western

RePlex with Total Protein Detection

To get the most data out of precious samples like brain homogenate, Simple Western eliminates the arduous strip and re-probe procedure by offering RePlex™, which efficiently removes the antibodies from the first round of probing for a second round of probing with fresh antibodies. The second round may also be used for Total Protein Detection, so you can normalize your data with confidence.

Right Figure: Total protein concentration was used as the internal loading control for protein quantification in tau transgenic mice. (A-B) Simple Western analysis of brain homogenate from tau transgenic mice using antibodies against Lamin A/C and b-Actin. (C) Simple Western Total Protein assay detects total protein in each brain homogenate sample. (D) Quantification of Lamin A/C, (E) b-Actin and (F) total protein concentration. (G) Representative electropherogram generated from the Simple Western Total Protein assay. Figure adapted with permission from Musi et al. (2018) Aging Cell

Brain Tissue Western Blot Data with Simple Western

Industry-Leading Fluorescence Detection Sensitivity

Implement Simple Western's Jess™ into your neuroscience research and development and get the most sensitivity from your brain tissue western blot. Stellar™ NIR/IR Detection Modules for Jess provide chemiluminescence and industry-leading fluorescence detection sensitivity along with excellent reproducibility and a 4-log dynamic range for advanced neuroscience experiments. Get advanced neuroscience results at detection levels below 1 pg that's 100X more sensitive than your average brain tissue western blot!

Webinar hero image with an artistic depiction of the Stellar technology

Dissociation of Mouse Neural Tissue for Single-Cell Western Analysis

In this App Note, we describe and characterize a protocol to successfully dissociate mouse neural tissue micro-surgically dissected from combined cortex, ventricular zone, and hippocampus regions of E18 mice into single cells. Using a multiplexed Single-Cell Western assay to analyze protein expression in the dissociated single neuronal cells, we identify 3 subpopulations of cells within the tissue: astrocytes, radial glia, and immature neurons based on expression of their respective phenotypic protein markers: GFAP, Pax6, and β-III tubulin.

Microdissected mouse brain tissue

Simple Western Neuroscience Application Spotlight Paper

Cutting-Edge Neuroscience Research

Simple Western has led to ground-breaking neuroscience research published in premier scientific journals.

Application Note for Simple Western application in neuroscience

Identify and Quantify Neural Cell Subtypes

Single-Cell Westerns can identify and quantify neural cell heterogeneity and monitor the differentiation of iPSCs into neural cell subtypes using GMP reagents.

Advances in Neuroscience eBook thumbnail

Advances in Neuroscience

Many scientists count on our advanced analytical solutions for the discovery and validation of biomarkers for conditions such as Parkinson’s, brain injury, Alzheimer’s, and more.

Customer Success Stories

Simple Western Applications

immune cells attack cancer cell



Transform your cancer & immuno-oncology research by utilizing next-generation Western blotting tools to give you richer views into your samples.

Solutions for Cell Signaling and Signal Pathways Analysis

Cell Signaling


Analyze entire signaling pathways with high sensitivity multiplexing and total protein normalization.

Testing for Lentivirus and AAVs

Cell and Gene Therapy


Next-generation analytical instrumentation to help improve and accelerate your cell and gene therapy workflows.

Advances in Targeted Protein Degradation

Targeted Degradation


Boost your screening studies using high throughput & hands-free Simple Western systems for quantitative analysis.

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Pharmacokinetic Analysis


High-throughput pharmacokinetic analysis with quantitative, high-sensitivity assays directly in complex lysates and tissue homogenates. 




A validated workflow for protein analysis in extracellular vesicle samples from the leaders in exosome isolation and high sensitivity Western blotting solutions.

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Vaccine Development


Improve reproducibility, quantitation, and time to market for your vaccine candidates.

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Increase your sensitivity and multiplexing capabilities with neural samples using automated Western blotting systems.

Simple Plex HEK-HCP Assay

Bioprocess Impurities


Screen for processing-related impurities and host-cell related contaminants. Quantify empty/full capsid content of viral vectors like AAVs.


SARS-CoV-2 Research


Reduce your lab time while accelerating your COVID‑19 research. Set up, leave and remotely review your data in just 3 hours!

SARS-CoV-2 Serology

SARS-CoV-2 Serology Assay


Rapid quantitative characterization of human IgG antibodies in serum or plasma reactive against multiple SARS-CoV-2 antigens.