Targeted Protein Degradation Western Blot Solutions

In this App Note, researchers performed protein degradation western blot analysis of 120 samples in just two overnight runs to provide highly quantitative data, which allows for an accurate determination of DC₅₀ and D_max. Researchers used off-the-shelf antibodies from Novus Biologicals and PROTAC^® degraders from Tocris to profile PROTAC^®-induced Cereblon neosubstrate degradation.

dTAG degradation technology offers a generalizable strategy to degrade, in principle, any intracellular protein of interest. Its broad applicability makes this a valuable strategy for exploring and validating targets using automated western blot protein degradation assays.

Do you want immunoassay-like quantitation for your western blot protein degradation analysis with low sample volume? Get picogram-level sensitivity with just 3 µL of starting material! Learn how the fully automated protein degradation assay on Simple Western is used in labs worldwide to quantify Degrader-induced protein knockdown. 

The team at Boehringer Ingelheim presents their recent real-world TPD project examples and in-depth analysis, covering insights on device and consumable management and optimized protein degradation assay setups to increase protein degradation western blot throughput.

Watch Dr. Gary Allenby, CSO at Aurelia Bioscience, who shares how their research team utilizes automated protein degradation western blot systems to rapidly and reproducibly quantify protein knockdown following treatment with Degraders.

Learn how western blot protein degradation assays with Simple Western reveal dCBP-1 protein degradation of p300/CBP in healthy and diseased cells from Christopher J. Ott, PhD, Harvard Medical School.

Bio-Techne offers a unique portfolio of high-quality reagents, instruments, and services for your protein degradation pathway research. Our TPD Research Product Guide highlights our tools and services to support your Targeted Protein Degradation research.

This edition of expert insights highlights advancements in the vibrant field of targeted protein degradation with small molecule drugs that aim for 'undruggable' targets. This eBook also connects researchers to new technologies accelerating drug discovery with robust high throughput screens and quantitative protein assays like the automated Simple Western™ platform from Bio-Techne.