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Analytical Solutions for Cell and Gene Therapy Research
Your vision is to create life-changing cell and gene therapies. But getting your cell therapy products to market quickly and efficiently requires accurate testing of critical attributes. That’s where Bio-Techne can help. Our broad portfolio of reliable analytical tools and platforms is designed to accelerate your workflow while providing unparalleled speed, accuracy, and reproducibility of results — from start to finish.
At Bio-Techne, we are fully committed to standing by you every step of the way. All of our analytical products and services are governed by rigorous quality assurance measures, helping to make sure your cell therapy products meet your precise quality, purity, and performance demands.
Cellular Phenotyping
Characterize your cell populations at every stage of the workflow using industry-leading Bio-Techne antibodies and highly sensitive and automated immunoassays. Quickly confirm that your cells exhibit the correct phenotype and meet your purity requirements. Whether you are looking for single analyte or multiplexing capabilities, we have your current and future needs covered.
Vector Characterization
Accurate titer measurement of vectors is critical for researchers studying infectious disease, vaccine development, and cell and gene therapy. Our highly reproducible assays and platforms provide fast, hands-free assessment of vectors like AAV and lentivirus, including identity, purity, stability, and other critical quality attributes. Rigorous quality control helps ensure our assays remain consistent and highly reproducible over time.
- Cell/lineage differentiation/inhibitory marker expression antibodies: Detect multiple cell surface and intracellular markers simultaneously at the single cell level using combinations of fluorophore-conjugated antibodies.
- Proteome Profiler™ Antibody Arrays: Blot-based, high throughput and semiquantitative, allowing measurement of more than 100 proteins for early-stage discovery
- ELISA Kits: Available in a range of formats including our optimized ready-to-use Quanitkine™ kits, 90-minute QuicKit™, and our flexible DuoSet™ ELISA options spanning over 1,000 targets.
- Luminex® Discovery Assays and Luminex High-Performance Assays: Discover and validate biomarker candidates with Luminex multiplex immunoassays. Profile multiple analytes in one sample. Customizable analyte menu available with over 450 analytes.
- Simple Plex™ assays: Automated quantitation of cytokine secretion profiles in just 90 minutes.
- Simple Western™ assays: Fully automated and quantitative Western analysis to detect biomarkers and process-related impurities in <3 hours.
- Multicolor flow cytometry kits: Antibodies targeting key surface and secreted proteins plus transcription factors.
- FluorokinesTM Fluorescent-labeled proteins: Highly specific human proteins for direct ligand-based cell analysis.
- Stem cell functional identification kits: Stem cell differentiation and verification kits help build consistent and robust cell culture models.
- Single-Cell Westerns: Analyze heterogeneity of cell type, activation status, and gene transfer efficiency.
- RNAscope™ ISH assays: Single-cell RNA expression analysis to localize your cell therapy in the target tissue.
- T cell monitoring: Reagents and assays to confirm that your cells don’t become over-activated.
- Simple Western™ Assays: Fully automated, high sensitivity Western characterization of AAV stability, identity, and purity with only 3 µL of sample.
- Maurice iCE Assays: Analytical instrument to determine the stability, identity, and purity of your AAV vectors and LNPs with absorbance and fluorescence detection.
- AAV and lentiviral viral titer assays: Fast and reproducible viral titer assays offer a highly effective method to quantify the viral titer of both lentivirus and adeno-associated virus capsids.
- Multicolor flow cytometry kits: Antibodies targeting key surface and secreted proteins plus transcription factors.
- Immune checkpoint blockade antibodies: Antibodies specificity confirmed with cells and lysates from an engineered knockout cell line.
- Single-Cell Westerns: Analyze heterogeneity of cell type, activation status, and gene transfer efficiency.
- DNAscope™ assay: Accurate and reliable detection of DNA aberrations and spatial arrangements of DNA.
Contaminant Analysis
Undetected process-related impurities can lead to reduced product efficacy, quality issues, and undesirable immune responses in patients. Whether you need to screen for host cell proteins or process residuals, our reliable, repeatable analytical solutions help drive confidence in the purity of your final product. Get the accuracy and specificity you need to detect co-migrating proteins, report molecular weight, and automate the entire analysis process.
Monitoring Efficacy and Toxicity
Excessive release of pro-inflammatory cytokines can be a toxic response to cell therapy. Our trusted antibodies and immunoassays allow you to generate accurate, reliable data, helping you get your therapies to patients safely and efficiently. Our automated ELISAs, Western blotting, and particle detection instruments, along with our breadth of flow cytometry antibodies, are ushering in a new era of time-efficient and highly sensitive analysis solutions.
- Simple Plex™ Assays: Automated quantitation of cytokine secretion profiles in just 90 minutes.
- Quantikine™ ELISA Kits: Choose from over 450 optimized and ready-to-use kits. Stringent quality control helps ensure our ELISA assays remain consistent and highly reproducible over time.
- Micro-Flow Imaging™: Characterizes and quantitates residual activation beads and protein aggregates.
- Simple Western™ Assays: Fully automated Western immunoassay and total protein analysis for bioprocess contaminant detection with superior sensitivity to SYPRO® Ruby total protein stains.
- Mycoplasma detection: High-throughput, routine screening of cell cultures for mycoplasma contamination.
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CHO Host Cell Protein Impurity Testing: Simple Plex assays automate the CHO HCP in-process testing workflow and provide high-quality consistent results.
- Multicolor flow cytometry kits: Antibodies targeting key surface and secreted proteins plus transcription factors.
- Quantikine™ and DuoSet™ ELISA Kits: Available in a range of formats. xtensively tested for long-term consistency and reproducibility. Flexible DuoSet kits are adaptable across multiple platforms.
- Luminex® Discovery Assays and Luminex High-Performance Assays: Discover and validate biomarker candidates with Luminex multiplex immunoassays. Multiplex up to 50 analytes in one sample. Customizable analyte menu available with over 450 analytes.
- Simple Plex™ Assays: Automated quantitation of cytokine secretion profiles in just 90 minutes.
- Simple Western™ Assays: Fully automated, Western-based immunoassays with results in as little as 3 hours.
- Single-Cell Westerns: Analyze heterogeneity of cell type, activation status, and gene transfer efficiency.
- Antibodies for disease biomarkers: Affinity-purified polyclonal antibodies and purified proteins for immunoassay development.
- Angiogenesis assays: Membrane-based antibody arrays for the parallel determination of the relative levels of selected angiogenesis-related proteins.
- RNAscope™ ISH technology: Single-cell RNA expression analysis to localize your cell therapy in the target tissue.
- Cell/lineage marker antibodies: Detect multiple cell surface and intracellular markers simultaneously at the single cell level using combinations of fluorophore-conjugated antibodies.
- AAV detection: Automated, high-throughput analytical platforms and systems let you characterize AAVs in sample volumes as low as 3 µL.
Evaluating CAR Expression on CAR-T Cells Using FluorokinesTM
Evaluating CAR Expression on CAR-T Cells Using FluorokinesTM
One of the current challenges in the field of CAR-T cell therapy is the lack of a simple assay to easily detect and quantify CAR expression following T cell transduction. Read this application note to learn about the advantages of using R&D SystemsTM Fluorokines fluorescent-labeled proteins to directly stain and detect CAR+ cells by flow cytometry.
The CAR ToolKit: Progress and Challenges in CAR-T/NK Cell R&D and Mfg
The CAR ToolKit: Progress and Challenges in CAR-T/NK Cell R&D and Mfg
With numerous clinical trials underway and multiple CAR-T cell products now approved, solving the current obstacles when manufacturing CAR-T/NK cell products continues to be a pressing issue for the cellular immunotherapy field. Watch this webinar, hosted by BioInsights, to learn about the current challenges, latest progress, and innovation facing CAR-T and CAR-NK cell development.
Quantify Secreted T Cell Activation Markers
Simple Plex assays on the Ella platform offer a rapid and precise assessment of secreted T Cell activation markers to boost cell therapy development and manufacturing efficiency. Get high-quality, quantitative data in just 90 minutes with unparalleled flexibility and ease of use.
Streamline Your Cell And Gene Therapy Process
Getting your therapeutic across the finish line is all about meeting critical quality and purity standards within an accelerated workflow. From viral vector analysis to research and clinical level cell characterization, our robust, automated analytical platforms will help get your therapeutic to patients quickly and safely.
Following the success of your initial research, we can provide large-scale quantities of critical, specialized reagents to assure your continued success. Work performed within our FDA-regulated facilities enables you to move seamlessly into regulatory agency submissions with our regulatory affairs team as a partner.
Featured Analytical Products
Next-Generation Automated ELISA
Simple Plex assays running on the fully automated Ella™ platform offer a viable alternative to traditional plate-based ELISAs for rapid multiplex analysis across a broad range of research and clinical applications.
cIEF and CE-SDS with Maurice
Characterize LNPs or viral vectors and assess vector purity and stability with a fully integrated CE platform that enables rapid analysis of charge and size heterogeneity.
Luminex® Multiplex Assays
Discover and validate biomarker candidates with Luminex Discovery and Luminex High-Performance multiplex immunoassays. Multiplex up to 50 analytes in one sample.
Multicolor Flow Cytometry
Our Multicolor Flow Cytometry kits contain fluorochrome-conjugated antibodies for simultaneous detection of up to four different target molecules on a single cell.
Fully Automated Simple Western
Simple Western™ automated multi-attribute analysis of viral and non-viral vectors
Micro-Flow Imaging
Gain more insight into the world of sub-visible particles with advanced Micro-Flow Imaging. Display images in real-time for on-the-spot visual verification and complete confidence in your results.
The Latest in Cell and Gene Therapy Analytics
Automated Analysis of Lentivirus and AAV
Learn how Simple Western, Simple Plex, and Maurice improve your viral vector analytics with rapid assessment of viral titer and purity analysis of lentivirus and AAV.
See What's Inside Your AAV Capsids
Explore the impact of stress on AAV charge separation profiles, gain key insights into DNA and protein packaging within the virus, and optimize the quality of AAV products for safer, more effective gene therapies.
Tools for Cell-Based Therapies
Learn about some of the latest tools and genome engineering services that can enhance your cell therapy manufacturing process and disrupt the status quo for manufacturing, validating, and monitoring cell therapies.
Customization for Cell Therapy
Learn how customization at the raw material level can help give cell therapy manufacturers confidence in the security of their supply, de-risk manufacturing processes, and improve scalability.
Improving Preclinical Gene Therapy
Learn how RNAscope™ and BaseScope™ in situ hybridization technologies are enabling more informative and efficient preclinical gene therapy development and contributing critical data in IND-enabling studies.
Rapid and Flexible Gene Delivery
Get an inside look at the benefits of using TcBuster, our non-viral gene transfer system that shortens your process timeline and cost and also delivers larger cargo sizes compared to virus-based methods.
Accelerate Your Therapeutic Discovery
Cell Expansion and Characterization
Our cell therapy platforms offer sensitive and specific assays that spare your precious samples, with the flexibility to determine efficacy, potency and safety throughout your manufacturing process. See how you can start combining and creating panels for your T cells.
Efficient Contaminant Screening
Micro-flow Imaging (MFI) gathers rich morphological data and uses powerful software tools to classify different particle types in solution. Likewise, our reliable analytical platforms can help you accurately identify and differentiate between potential sources of bioprocess contaminants to confirm the quality and stability of your therapeutic.
Gene Transfer Assessment
See how our Single-cell Western platforms can differentiate between hemoglobin subunits and measure their expression in single red blood cells. Commercial, off-the-shelf reagents provide added flexibility for your cell and gene therapy experiments.
Therapy Response Profiling
ProteinSimple platforms provide simple yet sophisticated solutions for biomarker and cell type profiling and characterization. From immuno-oncology and vaccines to cell therapy, explore ProteinSimple research applications by area of interest or by instrument.
This new eBook provides an overview of several biological and manufacturing challenges facing the development and production of T cell therapies. It follows with examples of how Bio-Techne products and services can address these challenges at each stage of the process.
Advance your cell and gene therapy research with flexible and innovative analytical tools and resources for every step of the manufacturing process. Our experienced team will work with you to develop, scale, and deliver exactly what you need to get your therapy to market.
Luminex® is a Registered Trademark of Luminex Corporation.
