Chroman 1

Name: Chroman 1
Brand: Tocris Bioscience
SKU: 7163

Catalog # 7163 | Tocris Bioscience a Bio-Techne Brand

COA / SDS

Highly potent and selective ROCK 2 inhibitor; improves cell survival after cryogenesis

Key Product Details

Description:	Highly potent and selective ROCK 2 inhibitor; improves cell survival after cryogenesis
Chemical Name:	(3S)-N-[2-[2-(Dimethylamino)ethoxy]-4-(1H-pyrazol-4-yl)phenyl]-3,4-dihydro-6-methoxy-2H-1-benzopyran-3-carboxamide dihydrochloride
Purity:	≥98% (HPLC)
Molecular Weight:	509.43

View Full Technical Details for Chroman 1

Technical Data
Citations / Reviews

Biological Activity for Chroman 1

Chroman 1 is a highly potent and selective ROCK2 (Rho-kinase) inhibitor (IC₅₀ values are 1 and 52 pM at ROCK2 and ROCK1, respectively). Chroman 1 exhibits >2000-fold selectivity for ROCK2 over a range of related kinases including MRCK, PKA, and AKT1 (IC₅₀ values are 150, >20000, and >20000 nM, respectively). Chroman 1 promotes survival of pluripotent stem cells (PSCs) in culture when used in combination with Emricasan (Cat. No. 7310), Polyamine Supplement x1000 (lyophilized) (Cat. No. 7739), and trans-ISRIB (Cat. No. 5284), a combination known as CEPT. When used as part of the CEPT cocktail, Chroman 1 also improves survival of differentiated cells following cryopreservation with cardiomyocyte cell survival increasing by 36% and motor neuron survival improving by 63% compared with DMSO controls.

Compound Libraries for Chroman 1

Chroman 1 is also offered as part of the Tocriscreen 2.0 Max, Tocriscreen Kinase Inhibitor Library and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for Chroman 1

M. Wt	509.43
Formula	C₂₄H₂₈N₄O₄.2HCl
Storage	Store at -20°C
Purity	≥98% (HPLC)
InChi Key	ROFMCPHQNWGXGE-SFHVURJKSA-N
Smiles	COC1=CC=C2OC[C@@H](C(NC3=C(C=C(C4=CNN=C4)C=C3)OCCN(C)C)=O)CC2=C1.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Chroman 1

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	50.94	100
	water	2.55	5

Preparing Stock Solutions for Chroman 1

The following data is based on the product molecular weight 509.43.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.96 mL	9.81 mL	19.63 mL
5 mM	0.39 mL	1.96 mL	3.93 mL
10 mM	0.20 mL	0.98 mL	1.96 mL
50 mM	0.04 mL	0.20 mL	0.39 mL

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References for Chroman 1

References are publications that support the biological activity of the product.

Chen A versatile polypharmacology platform promotes cytoprotection and viability of human pluripotent and differentiated cells. Nat.Methods 2021 PMID: 33941937
Chen Asymmetric synthesis of potent chroman-based Rho kinase (ROCK-II) inhibitors. Med.Chem.Commun.

Citations for Chroman 1

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