Coagulation is the formation of insoluble fibrin clots which help to prevent bleeding from damaged blood vessels. Deficiency in various components of the coagulation cascade results in bleeding disorders such as hemophilia and Von Willebrand disease. In contrast, pathological thrombosis is a life threatening complication of myocardial infarction, atherosclerosis, stroke, and vascular thromboembolism (VTE). Fibrinolytic therapy with Tissue Plasminogen Activator (tPA), Urokinase (uPA), or Streptokinase (SK) has shown efficacy at restoring blood flow in these emergencies.

Coagulation Cascade Proteases and Inhibitors

The backbone of the coagulation cascade is a system of serine proteases (coagulation factors and plasminogen activators) which are held in check by protease inhibitors (Serpins and Protein S). The endpoint of cascade activation is the cleavage of Fibrinogen by Thrombin and the crosslinking of Fibrin into the clot.

Coagulation Cascade Proteases and Inhibitors

Platelet Activation

The activation of platelets at sites of endothelial damage results in the release of many coagulation cascade factors and regulators. In addition, activated platelets express adhesion proteins which promote platelet aggregation and clot formation.

Platelet Activation in the Blood Stream During the Coagulation Activation Process

Multianalyte Immunoassays

These assay platforms let you detect many proteins in one experiment. They cover secreted factors, shed soluble receptors, and intracellular molecules. These assays are optimized for high sensitivity, low background, and minimal cross-reactivity.

Regulate Platelet Activation

Additional Resources from R&DSystems, a Bio-Techne Brand

"Atherosclerosis Disease Progression Poster" 
This poster covers healthy vascular function, endothelial damage, plaque progression, and plaque rupture.

"ProteinSimple® Analytical Instrumentation" 
Meet our protein characterization problem solvers – Simple Western™, Maurice, Simple Plex™, Single-Cell Western, and MFI™Systems.

"RNAscope® and BaseScope™ Assays"
Revolutionary ISH assays capture gene expression in situ at single cell level in intact tissue.

"Bioprocessing: From Start to Finish"
Instruments, GMP reagents, and services for clone selection through final product characterization.

"Cell and Gene Therapy Solutions"
Pioneering tools to simplify your workflow – cell separation, cell culture, and quality control.

"GMP Grade Proteins"
Cytokines and growth factors for ancillary use in preclinical to clinical studies.