CoraFluor™ 1, amine reactive New

Name: CoraFluor™ 1, amine reactive
Brand: Tocris Bioscience
SKU: 7920

Catalog # 7920 | Tocris Bioscience a Bio-Techne Brand

COA / SDS

Terbium cryptate FRET donor for TR-FRET assay development; amine reactive for conjugation

Key Product Details

Description:	Terbium cryptate FRET donor for TR-FRET assay development; amine reactive for conjugation
Molecular Weight:	1485.25

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Technical Data
Citations / Reviews

Biological Activity for CoraFluor™ 1, amine reactive

CoraFluor™ 1, amine reactive is a terbium-based time-resolved Förster resonance energy transfer (TR-FRET) donor for TR-FRET assay development, such as target engagement and ternary complex assays. This product is designed with a PFP ester for reactivity with primary amines, making it suitable for labeling biomolecules, including antibodies, nanobodies, proteins and peptides. It is exceptionally bright, providing unmatched assay sensitivity and stability in biological media over other commercially available TR-FRET donors. CoraFluor™ 1 amine reactive is compatible with common acceptor dyes such as FAM, TMR and Cyanine 5, SE (Cat. No. 5436), enabling seamless assay integration in high throughput screening applications.

Please refer to the protocol for further information on conjugation.

CoraFluor™ is a trademark of Bio-Techne Corp.

Licensing Information

Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Optical Data for CoraFluor™ 1, amine reactive

λ_abs	338 nm
Extinction Coefficient (ε)	23100 M^-1cm^-1
Quantum Yield (φ)	0.58
Closest Laser line	337 nm
Reactivity	Primary amines
Application	Time-resolved fluorescence energy transfer (TR-FRET)

Scientific Data Examples for CoraFluor™ 1, amine reactive

	CoraFluor™ 1 stability profile. Stability profiling of CoraFluor™ 1 and a commercially available TR-FRET donor (5 nM) after 7 days of exposure to 50 mM PBS, acetic acid (pH 3), sodium acetate (pH 5), MES (pH 6) or Tris buffer (pH 7.5) supplemented with various additives (DTPA, Ca²⁺, Mg²⁺, DTT, TCEP = 1 mM; Mn²⁺, Cu²⁺ = 0.5 mM).
	CoraFluor™ TR-FRET target engagement assay High throughput TR-FRET target engagement assay profiling BRD4 inhibitors and Degraders on endogenous BRD4 using a single detection mix consisting of a primary BRD4 antibody, a CoraFluor™ 1 labeled VHH secondary antibody, and a fluorescent JQ1-based tracer (JQ1-FITC Catalog #7722).

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data for CoraFluor™ 1, amine reactive

M. Wt	1485.25
Storage	Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

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References for CoraFluor™ 1, amine reactive

References are publications that support the biological activity of the product.

Payne A direct high-throughput protein quantification strategy facilitates discovery and characterization of a celastrol-derived BRD4 degrader. Cell Chem.Biol. 2022 PMID: 35649410
Payne Resolving the deceptive isoform and complex selectivity of HDAC1/2 inhibitors. Cell Chem.Biol. 2022 PMID: 35298895
Payne Bright and stable luminescent probes for target engagement profiling in live cells. Nat.Chem.Biol. 2021 PMID: 34675420

Citations for CoraFluor™ 1, amine reactive

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