Cyanine 5, SE

Name: Cyanine 5, SE
Brand: Tocris Bioscience
SKU: 5436

Catalog # 5436 | Tocris Bioscience a Bio-Techne Brand

Citations (1)

COA / SDS

Red fluorescent dye for the labeling of amines; supplied as NHS ester

Key Product Details

Description:	Red fluorescent dye for the labeling of amines; supplied as NHS ester
Alternative Names:	Cy5 NHS Ester
Chemical Name:	2-[5-[1-[6-[(2,5-Dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-1,3-dihydro-3,3-dimethyl-5-sulfo-2H-indol-2-ylidene]-1,3-pentadien-1-yl]-1-ethyl-3,3-dimethyl-5-sulfo-3H-indolium, inner salt
Purity:	≥95% (HPLC)
Molecular Weight:	753.88

View Full Technical Details for Cyanine 5, SE

Technical Data
Citations (1) / Reviews

Biological Activity for Cyanine 5, SE

Cyanine 5, SE is a red fluorescent dye for the labeling of amines. Excitation maximum ~649 nm; emission maximum ~666 nm.

For more information on how Cyanine 5, SE may be used, see our protocol: Conjugation Protocol for Amine Reactive Dyes.

Optical Data for Cyanine 5, SE

Emission Color	Red
Brightness
λ_abs	649 nm
λ_em	666 nm
Extinction Coefficient (ε)	250000 M^-1cm^-1
Quantum Yield (φ)	0.2
Closest Laser line	647 nm
Reactive Group	NHS ester
Reactivity	Primary amines
Cell Permeable	No
Correction Factor 260	0.03
Correction Factor 280	0.04
Application	Fluorescence Microscopy, Confocal Microscopy
Alternative For	Alexa Fluor 647; DyLight 649

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Technical Data for Cyanine 5, SE

M. Wt	753.88
Formula	C₃₇H₄₃N₃O₁₀S₂
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	146368-14-1
PubChem ID	118705155
InChi Key	WXWLHDCCGVWTDZ-UHFFFAOYSA-N
Smiles	CC1(C)C2=C(C=CC(S(=O)(O)=O)=C2)N(CCCCCC(ON3C(CCC3=O)=O)=O)/C1=C/C=C/C=C/C4=[N+](CC)C5=C(C=C(S(=O)([O-])=O)C=C5)C4(C)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Cyanine 5, SE

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	75.39	100
	DMF	75.39	100

Preparing Stock Solutions for Cyanine 5, SE

The following data is based on the product molecular weight 753.88.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.33 mL	6.63 mL	13.26 mL
5 mM	0.27 mL	1.33 mL	2.65 mL
10 mM	0.13 mL	0.66 mL	1.33 mL
50 mM	0.03 mL	0.13 mL	0.27 mL

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References for Cyanine 5, SE

References are publications that support the biological activity of the product.

Shin Live-cell imaging of Pol II promoter activity to monitor gene expression with RNA IMAGEtag reporters. Nucleic Acids Res. 2014 PMID: 24753407

Citations for Cyanine 5, SE (1)

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Citations are publications that use Bio-Techne products. Selected citations for Cyanine 5, SE include:

Erik A et al. (2022) Directed Evolution of Fluorescent Proteins in Bacteria. Methods Mol Biol. 2564:75-97
PMID: 36107338

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