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Supremely Flexible. Highly Reliable.

DuoSet™ ELISA Development Systems kits offer a reliable, economical alternative to purchasing a ready-to-use ELISA or separate antibodies. Each DuoSet kit contains carefully selected and validated matched antibody pairs for accurate measurement of recombinant and natural proteins in biological fluids.

A mass-calibrated recombinant standard helps reduce assay variability and ensure long-term consistency.

  • Highly specific and optimized for superior performance
  • Adaptable across multiple platforms
  • More than 1,000 targets with new and novel targets across multiple species
  • Low cost per data point 

Proven Quality. Consistent Performance.

Consistent ELISA Development Using Matched Antibody Pairs

Specific and Consistent

Carefully selected antibody pairs combined with a mass-calibrated standard help ensure accurate performance and consistent results for data collected over time.

Affordable ELISA Kits

Economical ELISA

Economical 5 and 15 plate kit formats provide a low cost per data point while convenient ancillary reagent kits eliminate the need to buy each ancillary reagent separately.  

Affordable ELISA Development Kits in Multiple Species Options

Large Menu Across Species

Select from an expansive DuoSet kit menu that includes more than 1,000 ELISAs across 16 different species.

High Quality Low Cost ELISA Assays

Exceptional Immunoassay Quality

Application of our renowned Quantikine™ ELISA development practices helps ensure accurate, reproducible results over a broad dynamic range.    

DuoSet ELISA Development Systems Assay Principle

DuoSet ELISA Procedure Steps showing steps and protocol to run R&D Systems DuoSet ELISA

DuoSet IC (Intracelluar) ELISA Development Systems

DuoSet IC (Intracellular) kits offer a fast, sensitive and economical method to detect and quantify intracellular factors in cell lysate samples. These signal transduction assays offer an attractive alternative to traditional Western Blot, providing up to 10 to 30 times more sensitivity.

DuoSet IC (Intracellular) Phospho-specific ELISA kits measure the amount of phosphorylated protein in cell lysates using activity using a specifically targeted detection antibody included in the kit. Targets include kinases, heat shock, apoptosis-related and genotoxic stress proteins.

DuoSet Supplemental Products

Supplemental products are available in a convenient Ancillary Reagent Kit for performing five 96-well assays. Each DuoSet Ancillary Reagent Kit provides a comprehensive collection of reagents and plates to assay five 96-well DuoSet ELISA plates. Choose components separately (in bulk sizes) for easy assay customization. 

DuoSet Ancillary Reagent Kits include:

  • 96-well Plates
  • Plate Sealers
  • 1-Step Color Reagent
  • Stop Solution
  • ELISA Plate-coating Buffer
  • Reagent diluent
  • Wash Buffer
DuoSet Ancillary Reagent - Kit - Card

Customer Success Stories

DuoSet ELISA Kit Applications



Explore novel targets with customizable DuoSet ELISA kits. Uncover new views into the role of proteins in cancer and accelerate protein-based therapeutics.   

Unique Animal Model Systems

Animal Model Systems

Choose from a large selection of DuoSet ELISA kits with analytes supporting a range of biological processes and 16 different model species.

Immunology Cell


Different immune cell types can be characterized based on their cytokine secretion profiles. DuoSet ELISAs contain all the components necessary to develop your own immunoassay.

green immune cell sending out cytokines

Immune Response Profiling

Bio-Techne has a broad selection of immunoassays for immune response profiling. Choose the immunoassay that best suits your needs.

Automated ELISA platform Ella runs single and multiplex assays for CGT research

Analytical Solutions for Cell and Gene Therapy

Rigorously characterize your products for the safest possible therapies. Our immunoassay platforms enable rapid and accurate analysis of protein contaminants and viral vectors.

How to Run an R&D Systems DuoSet ELISA

Learn How to Run a DuoSet ELISA Assay

Get step-by-step guidance on how to run DuoSet ELISA kit. DuoSet ELISA Development Systems include the basic components required to develop a sandwich ELISA, including matched antibody pairs, standard, and streptavidin HRP.

Your Comprehensive Guide to ELISAs

Explore Bio-Techne's wide range of ELISAs and gain deeper insight into types of ELISAs, ELISA controls, best practices, and troubleshooting. Learn about detection of molecules, sample preparation, and calculating results.

Bio-Techne ELISA Recognition and Awards

Best ELISA Reviews and Awards