ExCellerate™ Human NK Cell Expansion Media, Animal Component-Free

Name: ExCellerate™ Human NK Cell Expansion Media, Animal Component-Free
Brand: R&D Systems
SKU: CCM037

Catalog # CCM037 | R&D Systems, Inc. a Bio-Techne Brand

For potent and highly proliferative NK Cells.

Key Product Details

Features:	Animal Component-Free Media for the ex vivo expansion and culture of human Natural Killer lymphocytes.
Key Benefits:	Supports feeder- and serum-free expansion of NK cells Phenol red-free formulation Animal component-free media to support patient safety Supports NK cell expansion from PBMCs or purified CD3⁺-depleted PBMCs Protocol to support feeder-cell culture process Protocol to support feeder-free culture process

Setting the standard in quality research reagents

Technical Data
Citations / Reviews

Summary

Why Use ExCellerate™ Human NK Cell Expansion Media, Animal Component-Free?

Natural Killer (NK) cells play an important role in both the adaptive and innate immune responses that control infection, autoimmunity, and tumor immunosurveillance. Due to their intrinsic and non-specific anti-tumor activity, human NK cells have been employed as anti-cancer therapies. Robust expansion and maintenance of NK Cells is critical to facilitate NK cell therapy.

Versatility of ExCellerate Human NK Cell Expansion Media

ExCellerate Human NK Cell Expansion Media is optimized for NK cell expansion when used in combination with Recombinant Human Cytokines from R&D Systems; however, this versatile media also provides robust growth using a variety of cytokine combinations (IL-2, IL-15, IL-12, IL-18, and IL-21) and cell activation methods, including particle, bead-based, or plate-bound Human NKp46/NCR1 Antibody. The media supports both feeder-based and feeder-free expansion methods.

Kit Contents

1 L of ExCellerate Human NK Cell Expansion Media, Animal Component-Free

Product Specifications

Species

Human

Limitations

FOR LABORATORY RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

The safety and efficacy of this product in diagnostic or other clinical uses has not been established.
This reagent should not be used beyond the expiration date indicated on the CoA.
Results may vary due to variations among cells derived from different donors.

Scientific Data Examples

	NK Cell Fold Expansion. Purified NK Cells from PBMCs were grown with ExCellerate™ NK Cell Expansion Media, Animal Component-Free (Cat # CCM037), irradiated feeder cells, 5% HuAB Serum, and a cytokine cocktail of rhIL-2 (Cat # BT-002-AFL) and rhIL-15 (Cat # BT-015-AFL) over a 14-day culture.
	NK Cell Viability. Purified NK Cells from PBMCs were grown with ExCellerate™ NK Cell Expansion Media, Animal Component-Free (Cat # CCM037), irradiated feeder cells, 5% HuAB Serum, and a cytokine cocktail of rhIL-2 (Cat # BT-002-AFL) and rhIL-15 (Cat # BT-015-AFL) over a 14-day culture.
	NK Cell Killing Capacity. Purified NK Cells from PBMCs were grown with ExCellerate™ NK Cell Expansion Media, Animal Component-Free (Cat # CCM037), irradiated feeder cells, 5% HuAB Serum, and a cytokine cocktail of rhIL-2 (Cat # BT-002-AFL) and rhIL-15 (Cat # BT-015-AFL) over a 14-day culture.
	Phenotype analysis of 4 donors. Purified NK Cells from PBMCs were grown with ExCellerate™ NK Cell Expansion Media, Animal-Free (Cat # CCM037), irradiated feeder cells, 5% HuAB Serum, and a cytokine cocktail of rhIL-2 (Cat # BT-002-AFL) and rhIL-15 (Cat # BT-015-AFL) over a 14-day culture.
	Serum-Free NK Cell Fold Expansion. Purified NK Cells from PBMCs were grown in a Serum-Free process with ExCellerate™ NK Cell Expansion Media, Animal Component-Free (Cat # CCM037), irradiated feeder cells, and a cytokine cocktail of rhIL-2 (Cat # BT-002-AFL) and rhIL-15 (Cat # BT-015-AFL) over a 14-day culture.
	Serum-Free Process NK Cell Viability. Purified NK Cells from PBMCs were grown in a Serum-Free process with ExCellerate™ NK Cell Expansion Media, Animal Component-Free (Cat # CCM037), irradiated feeder cells, and a cytokine cocktail of rhIL-2 (Cat # BT-002-AFL) and rhIL-15 (Cat # BT-015-AFL) over a 14-day culture.
	Serum-Free Process Killing Capacity. Purified NK Cells from PBMCs were grown in a Serum-Free process with ExCellerate™ NK Cell Expansion Media, Animal Component-Free (Cat # CCM037), irradiated feeder cells, and a cytokine cocktail of rhIL-2 (Cat # BT-002-AFL) and rhIL-15 (Cat # BT-015-AFL) over a 14-day culture.
	Serum-Free Process Phenotype. Purified NK Cells from PBMCs were grown in a Serum-Free process with ExCellerate™ NK Cell Expansion Media, Animal Component-Free (Cat # CCM037), irradiated feeder cells, and a cytokine cocktail of rhIL-2 (Cat # BT-002-AFL) and rhIL-15 (Cat # BT-015-AFL) over a 14-day culture.
	Expanded Phenotype Analysis on Serum-Free Process. Purified NK Cells from PBMCs were grown in a Serum-Free process with ExCellerate™ NK Cell Expansion Media, Animal Component-Free (Cat # CCM037), irradiated feeder cells, and a cytokine cocktail of rhIL-2 (Cat # BT-002-AFL) and rhIL-15 (Cat # BT-015-AFL) over a 14-day culture.

Preparation & Storage

Shipping Conditions	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the recommended temperature. Protect from light.
Storage	Store the unopened product at -20 °C. Do not use past expiration date. Protect from Light.

Citations for ExCellerate™ Human NK Cell Expansion Media, Animal Component-Free

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