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GMP Small Molecules as Ancillary Reagents

Stem cells offer great potential for use as therapies in regenerative medicine. They can be used to generate cells to replace lost or unhealthy cells in a wide range of conditions including trauma, diabetes and Parkinson’s disease. The small molecules used as ancillary materials in the generation of stem cell therapies are required to be safe, reliable, consistent and quality-assured in order to ensure the safety of the stem cell therapy. Our GMP Small Molecules, manufactured according to cGMP guidelines, provide this assurance.

GMP Small Molecules

Product Name

Catalog #


CHIR 99021


GSK-3 Inhibitor and WNT pathway activator. Enables reprogramming of human somatic cells into iPSCs

LDN 193189


Potent and selective ALK2 and ALK3 inhibitor. Promotes neural induction of hPSCs

SB 431542


TGF Beta 1 (TGF-β1), ALK4 and ALK7 Inhibitor. Replaces SOX2 in reprogramming of fibroblasts to iPSCs

XAV 939


Tankyrase Inhibitor; inhibits WNT signaling. Promotes cardiomyocyte differentiation from ESCs



Selective ROCK Inhibitor. Increases survival of ESCs and iPSCs undergoing cryopreservation

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Learn about the quality standards and certifications for each of our GMP facilities as well as our quality control testing capabilities. We serve clients from around the world, and we guarantee the confidentiality of every contract.

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GMP Cell Manufacturing and Process Development

We offer process development services for any gene modified therapy, GMP patient processing for Phase I and Phase II clinical trials, and GMP manufacturing of iPSC cell lines.

GMP Small Molecule Resources

image of T Cell-Based Therapies eBook

eBook: T Cell-Based Therapies

This eBook outlines biological and manufacturing challenges facing T cell therapies. It follows with examples of how Bio-Techne products and services address these challenges. 

GMP Small Molecules Stem Cell Workflow Poster

Poster: Stem Cell Workflow Using Small Molecules

Our Tocris Stem Cell Workflow poster summarizes how small molecules have been used in protocols across the stem cell workflow, and highlights some of the key advantages of using small molecules. 

Stem Cells

Protocols: Key Stem Cell Protocol Highlights

Our Stem Cell Protocol snapshots highlight how small molecules may be used in place of or alongside proteins in cell culture, reprogramming and differentiation procedures. 

About GMP Small Molecules

GMP small molecules are important in the manufacture of stem cell therapies for translational research and clinical applications, where they are used as ancillary reagents. Ancillary reagents are utilized in the growth and manipulation of stem cells. Although they are not intended to be present in the final cell therapy product, they may influence its safety, so the use of GMP ancillary reagents is advisable.

The clinical phases of development of any therapy are highly regulated in order to mitigate the risk to patient safety, and this includes the use of GMP ancillary reagents (raw materials) for developing stem cell therapies. Our GMP small molecules are manufactured following relevant sections of ICH Q7 guidelines (Good Manufacturing Practice Guide for Active Pharmaceutical Ingredients), which ensures batch-to-batch consistency, traceability from starting material to final product and animal-free production.

View Our Cell and Gene Therapy Resources

Serum-Free and Animal-Free Cell Culture


Increase the consistency of your cell cultures as you approach translational studies for regenerative medicine and cell therapy programs. Adopting these media will

  • Reduce variability in media composition
  • Simplify compliance with regulatory guidelines
  • Simplify comparability testing for raw material changes

New! ExCellerate™ GMP iPSC Expansion Medium

For seamless transition from our equivalent RUO iPSC Expansion Medium. Both media support robust expansion and maintenance of pluripotent stem cell culture for enhanced consistency and reproducibility.

  • Completely animal-free
  • All-in-one formulation
  • Stable cell integrity over long term culture

Background Information