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Simple Western PK Assay

Simple Western™ is a fully automated capillary immunoassay. Separate proteins by capillary isoelectric focusing (cIEF) or capillary electrophoresis sodium dodecyl sulfate (CE-SDS) followed seamlessly by immunodetection directly in the capillary.

  • Reproducible quantification. Use CE-SDS to resolve proteins by size and quantify intact IgG, aggregates and fragments IC/EC50, LOD, LOQ, and more, with intra- and inter-assay CVs of <20%.
  • Add a Size- or Charge-base separation to your immunoassay. Use CE-SDS to resolve proteins by size and quantify intact IgG, aggregates and fragments. Use cIEF to quantify the main peak, acidic peak(s), and basic peak(s) of your antibody therapeutic.
Simple Western Instruments - Jess & Peggy Sue Hero

PK Analysis of Biosimilar Monoclonal Antibodies

  • No sample cleanup. Analyze highly complex samples like human serum without lengthy purification or concentration steps.
  • High throughput. Process up to 96 samples in one overnight run without user intervention.
  • High sensitivity. Highly sensitive immunodetection using as little as 3 µL of sample. Get up to 8 data points on the same 5 µL of sample. Multiplex to get more data points per sample with chemiluminescence and Stellar™ NIR/IR fluorescence detection with industry-leading sensitivity. Use RePlex™ to perform sequential immunoassays or Total Protein Detection on the same sample.
  • 3-4 log dynamic range. Larger than ELISA.
Simple Western Pharmacokinetic Biosimilar Monoclonal Antibody AN Thumbnail
Pharmacokinetic evaluation of therapeutic monoclonal antibodies and their biosimilars.

Combine CE-SDS/cIEF with Immunodetection

  ELISA CE-SDS cIEF Simple Western
Immunodetection Yes No No Yes
Charge separation No No Yes Yes
Size separation No Yes No Yes

Quantitative Protein Characterization in Complex Samples

The Simple Western PK assay on Peggy Sue™ combines the separation power of cIEF and CE-SDS assays with the sensitive and specific detection of an immunoassay. This combination places Simple Western in a unique position to study the pharmacokinetics of monoclonal antibodies and their biosimilars.

  • Minimal matrix effects. Following cIEF/CE-SDS separation, proteins are immobilized to the capillary and thoroughly washed prior to immunodetection, flushing the remaining sample matrix out of the capillary. Thus, immuno-probing on Simple Western occurs in an idealized environment rather than within the sample matrix environment, reducing matrix interference that can plague ELISAs while also providing high-resolution charge/size separation. 
  • Sensitive immunodetection. Detect small amounts (low pg) of target protein with conventional Western blot antibodies.
  • Upstream and downstream workflows. Obtain comparable charge separation profiles in crude samples from early stages of product development with Peggy Sue and later stages of purified samples with Maurice cIEF. For more information, refer to our Protocol on Method Transfer between Maurice and Peggy Sue.

Simple Western PK analysis provides a complete picture of antibody pharmacokinetics in human serum.

Simple Western charge separation diagram

Bioprocess Workflow Solutions from Start to Finish

Look to us to provide top-quality instrumentation, reagents, and services for your protein bioprocessing. We offer an outstanding product selection and the flexibility to meet your specifications.

  • Decades of experience in process development for cell culture and protein purification.
  • World class reagent and assay development.
  • Industry preferred technologies that simplify, automate, and advance protein analysis.
  • Proven record of customer satisfaction for bulk supply, custom solutions, and documentation.
  • Compliance with 21 CFR Part 11, ISO 13485:2016, USP <1043>, 21 CFR 820, and ISO 9001:2015 as applicable.
  • Absolute confidentiality
ProteinSimple Bioprocessing brochure for solutions for the entire bioprocessing workflow