Sign In / Register
Fluorogenic spontaneously blinking yellow-emitting dye; for SMLSM; λabsλem 526/550 nm
Loading...
Key Product Details
| Description: | Fluorogenic spontaneously blinking yellow-emitting dye; for SMLSM; λabsλem 526/550 nm |
| Alternative Names: | HM-JF526 NHS |
| Chemical Name: | 2,5-Dioxopyrrolidin-1-yl 3',6'-bis(3,3-difluoroazetidin-1-yl)-2',7'-difluoro-3H-spiro[isobenzofuran-1,9'-xanthene]-6-carboxylate |
| Purity: | ≥90% (HPLC) |
| Molecular Weight: | 645.51 |
Biological Activity for HM Janelia Fluor® 526, SE
HM Janelia Fluor® 526, SE is a fluorogenic spontaneously blinking green-emitting dye; hydroxymethyl derivative of JF526. Suitable for super-resolution imaging including dSTORM, STED and single-molecule localization spectroscopy (SMLSM). The spontaneous blinking properties of HM Janelia Fluor® 526, avoids the requirement for strongly reducing dSTORM buffers. Also suitable for conventional confocal microscopy and live cell imaging. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®.
To measure the absorbance spectrum of this dye we recommend the following solvent: TFE plus 0.1% TFA.
We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.
HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for HM Janelia Fluor® 526, SE
| Emission Color | Yellow |
|---|---|
| Brightness | |
| λabs | 526 nm |
| λem | 550 nm |
| Closest Laser line | 488 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | Yes |
| Application | dSTORM, SMLSM, STED, SIM, Confocal Microscopy |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Citations for HM Janelia Fluor® 526, SE
Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.
Bio-Techne Brands
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
Setting the standard in quality research reagents for over 30 years
A trusted leader in quality life science reagents
Your trusted supplier for innovative and high performance life science reagents
Proprietary systems and consumables for simpler, more quantitative and affordable protein analysis
Proprietary RNAscope® technology capable of detecting and quantifying RNA biomarkers in situ at single molecule sensitivity
A world leader in developing liquid biopsy based diagnostics