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Human 15‑PGDH/HPGD Antibody
Catalog # AF5660 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Human
Applications
Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Summary for Human 15‑PGDH/HPGD Antibody
Immunogen
E. coli-derived recombinant human 15-PGDH/HPGD
Met1-Gln266
Accession # P15428
Met1-Gln266
Accession # P15428
Specificity
Detects human 15-PGDH/HPGD in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human 15‑PGDH/HPGD Antibody
Detection of Human 15-PGDH/HPGD by Western Blot.
Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human 15-PGDH/HPGD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5660) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for 15-PGDH/HPGD at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Human 15‑PGDH/HPGD by Simple WesternTM.
Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for 15-PGDH/HPGD at approximately 34 kDa (as indicated) using 10 µg/mL of Goat Anti-Human 15-PGDH/HPGD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5660) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human 15‑PGDH/HPGD Antibody
Application
Recommended Usage
Simple Western
10 µg/mL
Sample: Human liver tissue
Sample: Human liver tissue
Western Blot
1 µg/mL
Sample: Human liver tissue
Sample: Human liver tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: 15-PGDH/HPGD
HPGD, or 15-hydroxyprostaglandin dehydrogenase, is a NAD+-linked dehydrogenase that oxidizes the hydroxyl group at position 15 of prostaglandins to a ketone, resulting in a loss of biological activity (1). HPGD is a major enzyme for the catabolism of prostaglandins. The enzyme is a member of the short-chain alcohol dehydrogenase family of enzymes (2). HPGD is a cytosolic enzyme expressed in most tissues, with highest expression levels in placenta, lung, and kidney (3). It is inhibited by aspirin and nonsteroidal anti-inflammatory drugs (4). Defects in HPGD are a cause of hypertrophic osteoarthropathy (5).
References
- Anggard, E. and B. Samuelsson (1964) J. Biol. Chem. 239:4097.
- Krook, M. et al. (1990) Biochemistry 29:738.
- Tai, H.H. (1976) Biochemistry 15:4586.
- Mak, O.T. et al. (1982) Biosci. Rep. 2:503.
- Uppal, S. et al. (2008) Nat. Genet. 40:789.
Long Name
15-Hydroxyprostaglandin Dehydrogenase
Alternate Names
15PGDH, HPGD, PGDH1, SDR36C1
Gene Symbol
HPGD
UniProt
Product Documents for Human 15‑PGDH/HPGD Antibody
Product Specific Notices for Human 15‑PGDH/HPGD Antibody
For research use only
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