Human APRIL/TNFSF13 Antibody

Catalog # AF884 | R&D Systems, Inc. a Bio-Techne Brand
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AF884
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human APRIL/TNFSF13 Antibody

Immunogen

E. coli-derived recombinant human APRIL/TNFSF13

Specificity

Detects human APRIL/TNFSF13 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) Fas Ligand, rhTNF-alpha, rhTRAIL, and rhTRANCE is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human APRIL/TNFSF13 Antibody

Human APRIL/TNFSF13 ELISA Standard Curve.

Recombinant Human APRIL/TNFSF13 protein was serially diluted 2-fold and captured by Mouse Anti-Human APRIL/TNFSF13 Monoclonal Antibody (MAB8844) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human APRIL/TNFSF13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF884) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Detection of APRIL/TNFSF13 in U937 Human Cell Line by Flow Cytometry.

U937 human lymphoma cell line was stained with Goat Anti-Human APRIL/TNFSF13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF884, filled histogram) or control antibody staining (AB-108-C, open histogram) followed by anti-Goat IgG PE-conjugated Secondary Antibody (F0107). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human APRIL/TNFSF13 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human APRIL/TNFSF13 Monoclonal Antibody (Catalog # MAB8844).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human APRIL/TNFSF13 DuoSet ELISA Kit (Catalog # DY884B) for convenient development of a sandwich ELISA.

Flow Cytometry

0.25 µg/106 cells
Sample: U937 human lymphoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Western Blot

0.1 µg/mL
Sample: Recombinant Human APRIL/TNFSF13 (Catalog # 884-AP)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 3 publications using AF884 in the following applications:

Formulation, Preparation and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: APRIL/TNFSF13

APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, and TRDL1, is a member of the TNF ligand superfamily (1). APRIL is synthesized as a 32 kDa type II transmembrane protein which is cleaved by furin in the Golgi to release a 17 kDa soluble molecule (2, 3). Secreted APRIL consists almost entirely of a single TNF homology domain (2, 3). Little or no transmembrane APRIL is expressed on the cell surface (3). Alternate splicing generates isoforms with short deletions at the N- or C-terminus (4). Human APRIL shares 85% aa sequence identity with mouse and rat APRIL. Among TNF superfamily ligands, BAFF shows the greatest sequence homology with APRIL, and the two proteins exhibit overlapping biological activities. APRIL promotes cellular proliferation and protects from apoptosis in normal and transformed cells (3, 5‑7). It is present in elevated amounts in a wide variety of cancers primarily due to expression by tumor-infiltrating neutrophils (4, 5, 7‑9). Both APRIL and BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, and BAFF additionally functions through BAFF R (6, 10, 11). A stretch of basic amino acids at the N-terminus of APRIL is required for its interaction with heparan sulfate proteoglycans (HSPGs) (12, 13). Binding to HSPGs is independent of APRIL’s binding to TACI and BCMA (12, 13). Interaction with HSPGs serves to concentrate APRIL on the surface of cells, thereby favoring TACI- or BCMA-mediated effects (8, 9, 13). APRIL can form bioactive heterotrimers with BAFF, and these circulate in the serum of patients with rheumatic immune disorders (14). A bioactive protein known as TWE-PRIL consists of the intracellular domain, transmembrane segment, and stalk region of TWEAK fused to the TNF homology domain of APRIL (15). TWE-PRIL is expressed in monocytes and activated T cells, and in contrast to APRIL, it is presented on the cell surface (15).

References

  1. Planelles, L. et al. (2008) Curr. Mol. Med. 8:829.
  2. Hahne, M. et al. (1998) J. Exp. Med. 188:1185.
  3. Lopez-Fraga, M. et al. (2001) EMBO Reports 2:945.
  4. Kelly, K. et al. (2000) Cancer Res. 60:1021.
  5. Roth, W. et al. (2001) Cell Death Differ. 8:403.
  6. Yu, G. et al. (2000) Nat. Immunol. 1:252.
  7. He, B. et al. (2004) J. Immunol. 172:3268.
  8. Schwaller, J. et al. (2007) Blood 109:331.
  9. Mhawech-Fauceglia, P. et al. (2008) Eur. J. Cancer 44:2097.
  10. Day, E.S. et al. (2005) Biochemistry 44:1919.
  11. Bossen, C. et al. (2006) J. Biol. Chem. 281:13964.
  12. Hendriks, J. et al. (2005) Cell Death Differ. 12:637.
  13. Ingold, K. et al. (2005) J. Exp. Med. 201:1375.
  14. Roschke, V. et al. (2002) J. Immunol. 169:4314.
  15. Pradet-Balade, B. et al. (2002) EMBO J. 21:5711.

Long Name

A Proliferation-inducing Ligand

Alternate Names

CD256, TALL2, TNFSF13, TRDL1, ZTNF2

Entrez Gene IDs

8741 (Human); 69583 (Mouse); 287437 (Rat)

Gene Symbol

TNFSF13

Product Documents for Human APRIL/TNFSF13 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human APRIL/TNFSF13 Antibody

For research use only

Citations for Human APRIL/TNFSF13 Antibody (3)

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