Human B7‑1/CD80 Antibody

Name: Human B7‑1/CD80 Antibody
Brand: R&D Systems
SKU: AF140

Catalog # AF140 | R&D Systems, Inc. a Bio-Techne Brand

Citations (8)

Product Datasheet / COA / SDS

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Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human B7‑1/CD80 Antibody

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human B7-1/CD80
Extracellular domain

Specificity

Detects human B7-1/CD80 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human B7-2 and recombinant mouse B7-2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human B7‑1/CD80 Antibody

Detection of Human B7‑1/CD80 by Western Blot.

Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human B7-1/CD80 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF140) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for B7-1/CD80 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

B7‑1/CD80 in Human Tonsil.

B7-1/CD80 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human B7-1/CD80 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF140) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes in germinal center. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human B7‑1/CD80 Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cross-section through blood vessel) and human tonsil

Western Blot

1 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 8 publications using AF140 in the following applications:

ELISA Development (1 Publication)
Flow Cytometry (1 Publication)
ICC (1 Publication)
IHC (1 Publication)
IHC-Fr (1 Publication)
IHC-P (2 Publications)
Western Blot (1 Publication)

Formulation, Preparation and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: B7-1/CD80

B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T and B cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the
down‑regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up‑regulated through IFN-gamma. B7-1 and B7-2 are both members of the Immunoglobulin superfamily. Human B7-1 is a 288 amino acid (aa) protein containing a 34 aa signal peptide, a 208 aa extracellular domain, a 21 aa transmembrane domain, and a 25 aa cytoplasmic domain. Human B7-1 and B7-2 share 26% amino acid identity. Human and mouse B7-1 share 44% amino acid identity. However, it has been observed that both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and CTLA-4, suggesting that there are conserved amino acids which form theB7-1/B7-2/CD28/CTLA-4 critical binding sites.

References

Azuma, M. et al. (1993) Nature 366:76.
Freeman, G.J. et al. (1993) Science 262:909.
Freeman, G. et al. (1991) J. Exp. Med. 174:625.
Selvakumar, A. et al. (1993) Immunogenetics 38:292.
Chen, C. et al. (1994) J. Immunol. 152:4929.
Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.

Alternate Names

B71, CD80

Entrez Gene IDs

102140078 (Cynomolgus Monkey); 941 (Human); 12519 (Mouse); 25408 (Rat)

Gene Symbol

CD80

Product Documents for Human B7‑1/CD80 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human B7‑1/CD80 Antibody

For research use only

Citations for Human B7‑1/CD80 Antibody (8)

Showing 1 - 8 of 8 publications Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human B7‑1/CD80 Antibody include:

Species: Human
Sample Types: Whole Tissue
Applications: IHC

P Chen et al. (2020-09-08), Usefulness of the cytokines expression of Th1/Th2/Th17 and urinary CD80 excretion in adult-onset minimal change disease PeerJ, 2020-08(0):e9854.
PMID: 33194357
Species: Human
Sample Types: Whole Cells
Applications: ICC

T Hirano et al. (2018-03-23), Peripheral alveolar nitric oxide concentration reflects alveolar inflammation in autoimmune pulmonary alveolar proteinosis ERJ Open Res, 2018-04(1).
PMID: 29577041
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

P Ai et al. (2017-06-22), Tumor microenvironment contributes to Epstein-Barr virus anti-nuclear antigen-1 antibody production in nasopharyngeal carcinoma Oncol Lett, 2017-014(2):2458-2462.
PMID: 28781683
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Mulder DJ et al. (2011-02-01), Antigen presentation and MHC class II expression by human esophageal epithelial cells: role in eosinophilic esophagitis. Am. J. Pathol., 2011-0178(2):744-53.
PMID: 21281807
Species: Human
Sample Types: Urine
Applications: Western Blot

Garin EH et al. (2010-05-19), Urinary CD80 is elevated in minimal change disease but not in focal segmental glomerulosclerosis. Kidney Int., 2010-078(3):296-302.
PMID: 20485332
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Kuhne M et al. (2009-06-01), HLA-B27-restricted antigen presentation by human chondrocytes to CD8+ T cells: potential contribution to local immunopathologic processes in ankylosing spondylitis. Arthritis Rheum., 2009-060(6):1635-46.
PMID: 19479861
Species: Human
Sample Types: Plasma
Applications: ELISA Development

Sakthivel P et al. (2007-04-01), The soluble forms of CD28, CD86 and CTLA-4 constitute possible immunological markers in patients with abdominal aortic aneurysm. J. Intern. Med., 2007-0261(4):399-407.
PMID: 17391115
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr

Reiser J et al. (2004-05-01), Induction of B7-1 in podocytes is associated with nephrotic syndrome. J. Clin. Invest., 2004-0113(10):1390-7.
PMID: 15146236

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