Human BAFF/BLyS/TNFSF13B Antibody
Catalog # AF124 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Summary for Human BAFF/BLyS/TNFSF13B Antibody
Immunogen
Ala134-Leu285
Accession # Q9Y275
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human BAFF/BLyS/TNFSF13B Antibody
Cell Proliferation Induced By BAFF/BLyS/TNFSF13B and Neutralization by Human BAFF/BLyS/TNFSF13B Antibody.
In the presence of Goat F(ab')2 Anti-mouse IgM (1 µg/mL), Recombinant Human BAFF/BLyS/TNFSF13B (Catalog # 2149-BF) stimulates proliferation in mouse B cells in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Human BAFF/BLyS/TNFSF13B (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human BAFF/BLyS/TNFSF13B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF124). The ND50 is typically 3-12 ng/mL.BAFF/BLyS/TNFSF13B in Human Spleen.
BAFF/BLyS/TNFSF13B was detected in formalin fixed paraffin-embedded sections of human spleen using 15 µg/mL Goat Anti-Human BAFF/BLyS/TNFSF13B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF124) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human BAFF/BLyS/TNFSF13B Antibody
Immunohistochemistry
Sample: Formalin fixed paraffin-embedded sections of human spleen
Western Blot
Sample: Recombinant Human BAFF/BLyS/TNFSF13B (Catalog # 2149-BF)
Neutralization
Reviewed Applications
Read 1 review rated 5 using AF124 in the following applications:
Published Applications
Read 11 publications using AF124 in the following applications:
Formulation, Preparation and Storage
Purification
Endotoxin Level
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BAFF/BLyS/TNFSF13B
BAFF (also known as TALL-1, BLyS, and THANK) is a type II transmembrane glycoprotein belonging to the TNF superfamily and has been designated as TNF superfamily member 13B (TNFSF13B). Human BAFF is a 285 amino acid (aa) protein consisting of a 218 aa extracellular domain, a 21 aa transmembrane region and a 46 aa cytoplasmic tail (1, 2). BAFF has the typical structural characteristics of the TNF superfamily ligands. It is a homotrimeric protein having the structurally conserved motif known as TNF homology domain (3, 4). A higher ordered structure composed of a cluster of trimeric units resembling the structure of a viral capsid has also been reported (4). Human BAFF may be shed from the cell surface by proteolytic cleavage between R133 and Ala134 to yield a soluble form of the protein that is detectable in serum (1, 5). Within the TNF superfamily BAFF shares the highest homology (48%) with APRIL (1). BAFF shares with APRIL the ability to bind to BCMA and TACI and also binds specifically to BAFF receptor (BAFF R, also known as BR3 or TNFSFR13C), which is the principal BAFF receptor (6‑8). All three receptors are type III transmembrane proteins that are expressd in B cells. BAFF and APRIL can form active heteromers that bind to TACI (9). BAFF is expressed in peripheral blood mononuclear cells, in spleen and lymph nodes. Its expression in resting monocytes is up-regulated by IFN-alpha, IFN-beta, LPS and IL-10. BAFF provides critical survival signals to a subset of B cells with intermediate maturation status (T2 B cells) during the immune response (10). BAFF also plays an important role in the development of lymphoid tissue and enhances the survival of activated memory B cells (7, 11). Human and mouse BAFF share 86% aa sequence identity (1).
References
- Schneider, P. et al. (1999) J. Exp. Med. 189:1747.
- Mukhopadhyay, A. et al. (1999) J. Biol. Chem. 274:15978.
- Karpusas, M. et al. (2002) J. Mol. Biol. 315:1145.
- Liu, Y. et al. (2002) Cell 108:383.
- Cheema, G.S. et al. (2001) Arthr. Rheum. 44:1313.
- Marsters, S.A. et al. (2000) Curr. Biol. 10:785.
- Thompson, J.S. et al. (2001) Science 293:2108.
- Ng, L.G. et al. (2004) J. Immunol. 173:807.
- Roschke, V. et al. (2002) J. Immunol. 169:4314.
- Batten, M. et al. (2000) J. Exp. Med. 192:1453.
- Avery, D.T. et al. (2003) J. Clin. Invest. 112:286.
Long Name
Alternate Names
Gene Symbol
UniProt
Product Documents for Human BAFF/BLyS/TNFSF13B Antibody
Product Specific Notices for Human BAFF/BLyS/TNFSF13B Antibody
For research use only
Citations for Human BAFF/BLyS/TNFSF13B Antibody (11)
Citations are publications that use Bio-Techne products. Selected citations for Human BAFF/BLyS/TNFSF13B Antibody include:
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Species: Human
Sample Types: Whole Cells
Applications: NeutralizationH Talbot et al. (2020-07-28), BDNF belongs to the nurse-like cell secretome and supports survival of B chronic lymphocytic leukemia cells Sci Rep, 2020-010(1):12572.
PMID: 32724091 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCY Cao et al. (2019-12-06), BAFF is involved in the pathogenesis of IgA nephropathy by activating the TRAF6/NF?&kappaB signaling pathway in glomerular mesangial cells Mol Med Rep, 2019-121(2):795-805.
PMID: 31974601 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationM Imbrechts et al. (2018-08-01), IFN-? stimulates CpG-induced IL-10 production in B cells via p38 and JNK signalling pathways Eur. J. Immunol., 2018-00(0).
PMID: 30004580 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationO Berggren et al. (2017-08-28), Plasmacytoid dendritic cells and RNA-containing immune complexes drive expansion of peripheral B cell subsets with an SLE-like phenotype PLoS ONE, 2017-012(8):e0183946.
PMID: 28846748 -
Species: Human
Sample Types: Whole Tissue
Applications: IHC-PHamada T et al. (2015-09-30), Serum B cell-activating factor (BAFF) level in connective tissue disease associated interstitial lung disease. BMC Pulm Med, 2015-015(0):110.
PMID: 26424433 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCAbe T et al. (2015-07-06), The B Cell-Stimulatory Cytokines BLyS and APRIL Are Elevated in Human Periodontitis and Are Required for B Cell-Dependent Bone Loss in Experimental Murine Periodontitis. J Immunol, 2015-0195(4):1427-35.
PMID: 26150532 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationSaulep-Easton D et al. (2015-07-03), The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells. Leukemia, 2015-030(1):163-72.
PMID: 26139429 -
Species: Human
Sample Types: Cell Lysates,Whole Cells
Applications: ICC,Western BlotZhu J et al. (2012-03-31), BlyS is up-regulated by hypoxia and promotes migration of human breast cancer cells. J. Exp. Clin. Cancer Res., 2012-031(0):31.
PMID: 22463935 -
Species: Human
Sample Types: Whole Cells
Applications: ICCKuo SH et al. (2009-08-18), Unusual presentation of multiple pathologic bone fractures in a patient with gastric mucosa-associated lymphoid tissue lymphoma. Ann. Hematol., 2009-089(4):431-6.
PMID: 19688210 -
Species: Porcine
Sample Types: Whole Cells
Applications: ICCBergamin F et al. (2007-11-01), Essential role of antigen-presenting cell-derived BAFF for antibody responses. Eur. J. Immunol., 2007-137(11):3122-30.
PMID: 17935087 -
Species: Human
Sample Types: Whole Tissue
Applications: IHC-PElsawa SF et al. (2005-11-22), B-lymphocyte stimulator (BLyS) stimulates immunoglobulin production and malignant B-cell growth in Waldenstrom macroglobulinemia. Blood, 2005-1107(7):2882-8.
PMID: 16304043
There are no citations that match your criteria.
Customer Reviews for Human BAFF/BLyS/TNFSF13B Antibody (1)
Customer Images
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Application: ImmunohistochemistrySample Tested: Spleen tissueSpecies: HumanVerified Customer | Posted 02/18/2022Heat-retrieval in high-pH buffer primary Ab 10ug/mL for 60min ImmPRESS polymer HRP and DAB
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