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Human CCL3/MIP‑1 alpha Antibody
Catalog # AF-270-NA | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Human
Applications
ELISA Capture (Matched Antibody Pair), Immunohistochemistry, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Summary for Human CCL3/MIP‑1 alpha Antibody
Immunogen
E. coli-derived recombinant human CCL3/MIP-1 alpha
Ala27-Ala92
Accession # P10147
Ala27-Ala92
Accession # P10147
Specificity
Detects human CCL3/MIP‑1 alpha in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human (rh) MIP-1 beta, rhMIP-1 delta, rhMIP-3 alpha, rhMIP-3 beta, and recombinant mouse (rm) MIP-1 alpha is observed. Neutralizes the biological activity of recombinant human CCL3/MIP‑1 alpha. In the chemotaxis assay this antibody will also partially neutralize the biological activity of rhMIP-1 beta at a 20 fold higher IgG concentration. It will not neutralize the biological activity of rmMIP-1 beta or rmMIP-1 alpha.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human CCL3/MIP‑1 alpha Antibody
Chemotaxis Induced by CCL3/MIP‑1 alpha and Neutral-ization by Human CCL3/ MIP‑1 alpha Antibody.
Recombinant Human CCL3/MIP-1a (Catalog # 270-LD) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL3/MIP-1a (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL3/MIP-1a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-270-NA). The ND50 is typically 2-8 µg/mL.
CCL3/MIP‑1 alpha in Human Tonsil.
CCL3/MIP-1a was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human CCL3/MIP-1a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-270-NA) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human CCL3/MIP‑1 alpha Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil
Sample: Immersion fixed paraffin-embedded sections of human tonsil
Western Blot
0.1 µg/mL
Sample: Recombinant Human CCL3/MIP‑1 alpha isoform LD78a (Catalog # 270-LD)
Sample: Recombinant Human CCL3/MIP‑1 alpha isoform LD78a (Catalog # 270-LD)
Neutralization
Measured by its ability to neutralize CCL3/MIP‑1 alpha-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 2-8 µg/mL in the presence of 0.1 µg/mL Recombinant Human CCL3/MIP‑1 alpha isoform LD78a.
Human CCL3/MIP-1 alpha Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 5 using AF-270-NA in the following applications:
Published Applications
Read 12 publications using AF-270-NA in the following applications:
Formulation, Preparation and Storage
Purification
Antigen Affinity-purified
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL3/MIP-1 alpha
The macrophage inflammatory proteins -1 alpha and -1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP-1 alpha refers to the products of several independently cloned cDNAs, including LD78, pL78, pAT464, and GOS19. These cDNAs all code for the same human protein that is a homologue of the murine MIP-1 alpha. Mature MIP-1 alpha and MIP-1 beta in both human and mouse share approximately 70% homology at the amino acid level. The MIP‑1 proteins are members of the beta (C-C) subfamily of chemokines.
Both MIP-1 alpha and MIP-1 beta are monocyte chemoattractants in vitro. Additionally, the MIP-1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with MIP-1 alpha and MIP-1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. MIP-1 alpha has also been shown to attract B cells as well as eosinophils. MIP-1 proteins have been reported to have multiple effects on hematopoietic precursor cells and MIP-1 alpha has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. The functional receptor for MIP-1 alpha has been identified as CCR1 and CCR5.
Product Documents for Human CCL3/MIP‑1 alpha Antibody
Product Specific Notices for Human CCL3/MIP‑1 alpha Antibody
For research use only
Citations for Human CCL3/MIP‑1 alpha Antibody (12)
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Citations are publications that use Bio-Techne products. Selected citations for Human CCL3/MIP‑1 alpha Antibody include:
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Species: Human
Sample Types: Whole Cells
Applications: NeutralizationT Kodama et al. (2020-05-26), CCL3-CCR5 axis contributes to progression of esophageal squamous cell carcinoma by promoting cell migration and invasion via Akt and ERK pathways Lab. Invest., 2020-00(0).
PMID: 32457351 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryT Leng et al. (2019-09-17), TCR and Inflammatory Signals Tune Human MAIT Cells to Exert Specific Tissue Repair and Effector Functions Cell Rep, 2019-028(12):3077-3091.e5.
PMID: 31533032 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationWalker W et al. (2015-03-04), Increased Levels of Macrophage Inflammatory Proteins Result in Resistance to R5-Tropic HIV-1 in a Subset of Elite Controllers. J Virol, 2015-089(10):5502-14.
PMID: 25740989 -
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA DevelopmentWu W et al. (2009-10-07), Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin. J. Immunol., 2009-1183(9):5799-806.
PMID: 19812208 -
Species: Human
Sample Types: Urine
Applications: Antibody Array DevelopmentHu H et al. (2009-06-27), Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine. Transplantation, 2009-087(12):1814-20.
PMID: 19543058 -
Species: Human
Sample Types: Cell Culture Supernates
Applications: Luminex DevelopmentBiancotto A et al. (2008-03-01), Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo. AIDS Res. Hum. Retroviruses, 2008-024(3):453-62.
PMID: 18327985 -
Species: Human
Sample Types: Plasma
Applications: ELISA DevelopmentQui H et al. (2007-11-01), Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines. Clin. Chem., 2007-153(11):2010-2.
PMID: 18030696 -
Species: Human
Sample Types: Serum
Applications: Luminex DevelopmentPfleger C et al. (2007-10-22), Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays. J. Immunol. Methods, 2007-1329(1):214-8.
PMID: 17964594 -
Species: Human
Sample Types: Cell Culture Supernates
Applications: Luminex DevelopmentBiancotto A et al. (2007-02-08), Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1. Blood, 2007-0109(10):4272-9.
PMID: 17289812 -
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA DevelopmentMasih-Khan E et al. (2006-07-18), MIP-1alpha (CCL3) is a downstream target of FGFR3 and RAS-MAPK signaling in multiple myeloma. Blood, 2006-0108(10):3465-71.
PMID: 16849642 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationTownson JR et al. (2002-10-01), Gene copy number regulates the production of the human chemokine CCL3-L1. Eur. J. Immunol., 2002-132(10):3016-26.
PMID: 12355456 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationBinninger-Schinzel D et al. (2002-03-01), Simian immunodeficiency viruses with defective nef genes show increased susceptibility to the noncytotoxic antiviral activity of CD8+ lymphocytes. 2002-0294(1):209-21.
PMID: 11886279
There are no citations that match your criteria.
Customer Reviews for Human CCL3/MIP‑1 alpha Antibody (2)
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Application: ELISASample Tested: Cell culture supernatantSpecies: HumanVerified Customer | Posted 09/07/2019
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Application: ELISASample Tested: RAW 264.7 mouse monocyte/macrophage cell line and Cell LysatesSpecies: HumanVerified Customer | Posted 08/10/2019
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