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Human Chromogranin A Antibody
Catalog # MAB90981 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 948128
Product Summary for Human Chromogranin A Antibody
Immunogen
Human embryonic kidney cell line HEK293-derived human Chromogranin A
Met1-Gly457
Accession # P10645
Met1-Gly457
Accession # P10645
Specificity
Detects human Chromogranin A in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Chromogranin A Antibody
Chromogranin A in H69AR Human Cell Line.
Chromogranin A was detected in immersion fixed H69AR human small cell lung cancer cell line using Mouse Anti-Human Chromogranin A Monoclonal Antibody (Catalog # MAB90981) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Chromogranin A in Human Parathyroid.
Chromogranin A was detected in immersion fixed paraffin-embedded sections of human parathyroid using Mouse Anti-Human Chromogranin A Monoclonal Antibody (Catalog # MAB90981) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to principal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Chromogranin A Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed H69AR human small cell lung cancer cell line
Sample: Immersion fixed H69AR human small cell lung cancer cell line
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human parathyroid
Sample: Immersion fixed paraffin-embedded sections of human parathyroid
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB90981 in the following applications:
Published Applications
Read 1 publication using MAB90981 in the following applications:
Formulation, Preparation and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Chromogranin A
Product Documents for Human Chromogranin A Antibody
Product Specific Notices for Human Chromogranin A Antibody
For research use only
Citations for Human Chromogranin A Antibody (1)
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Citations are publications that use Bio-Techne products. Selected citations for Human Chromogranin A Antibody include:
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Species: Mouse
Sample Types: Cell Lysates,Whole Cells
Applications: Western Blot,ICCH Chen et al. (2020-09-03), Loss of MAGEL2 in Prader-Willi syndrome leads to decreased secretory granule and neuropeptide production JCI Insight, 2020-05(17).
PMID: 32879135
There are no citations that match your criteria.
Customer Reviews for Human Chromogranin A Antibody (1)
5 out of 5
1 customer
rating
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Application: ImmunohistochemistrySample Tested: Prostatic adenocarcinoma tissue and Tumor tissueSpecies: HumanVerified Customer | Posted 12/07/2021
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FAQs for Human Chromogranin A Antibody
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Q: I have following question about antibodies against Chromogranin A. Your products list the species but canine is not included. Does it mean that the products cannot be used on canine tissues?
A: When we do not list a species, it means we have not tested an antibody to work with those samples. If we test an antibody and find that it does not work, we will list the species with a minus sign. Unfortunately, we haven't tried these abs on canine tissues and they may work, but we cannot guarantee it. If you would like to purchase one to try, you will be eligible for the Innovators Reward Program.
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