Human E-Cadherin Quantikine ELISA Kit

Name: Human E-Cadherin Quantikine ELISA Kit
Brand: R&D Systems
SKU: DCADE0B

Catalog # DCADE0B | R&D Systems, Inc. a Bio-Techne Brand

Citations (9)

Product Datasheet / COA / SDS

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Key Product Details

Assay Length:	3.5 hours
Sample Type & Volume Required Per Well:	Cell Culture Supernates (100 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL), Urine (10 uL)
Sensitivity:	0.126 ng/mL
Assay Range:	0.625 - 20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Human E-Cadherin Quantikine ELISA Kit Summary:	The Quantikine™ Human E-Cadherin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human E-Cadherin in cell culture supernates, serum, plasma, saliva, and urine. It contains HEK293-expressed recombinant human E-Cadherin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human E-Cadherin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human E-Cadherin.

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Technical Data
Citations (9) / Reviews

Product Specifications for Human E-Cadherin Quantikine ELISA Kit

Measurement	Quantitative ELISA
Detection Method	Colorimetric - 450nm (TMB)
Conjugate	HRP
Reactivity	Human
Specificity	Natural and recombinant human E-Cadherin.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	2.42	7.06	14.6	2.19	6.55	14.2
Standard Deviation	0.047	0.145	0.266	0.136	0.368	0.845
CV%	1.9	2.1	1.8	6.2	5.6	6

Recovery

The recovery of human E-Cadherin spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=30)	242	131-479
Heparin Plasma (n=30)	236	116-479
Saliva (n=10)	145	21.4-278
Serum (n=30)	250	127-492
Urine (n=10)	60	3.03-121

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human E-Cadherin were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Examples for Human E-Cadherin Quantikine ELISA Kit

	Human E-Cadherin ELISA Standard Curve

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: E-Cadherin

The cadherin superfamily comprises a large number of cell surface glycoproteins with one or more cadherin repeats, which are involved in Ca2⁺-dependent cell-cell adhesion. Classical cadherins contain five extracellular cadherin repeats and a conserved cytoplasmic region that interacts with the actin cytoskeleton via catenins. In addition to cell adhesion, cadherins also mediate morphogenesis, cytoskeletal organization, and cell migration. In addition to the classical cadherins, the cadherin superfamily also includes desmosomal cadherins, protocadherins, 7TM cadherins, FAT family cadherins, and T-cadherin.

Item	Value
Entrez Gene IDs	999 (Human); 12550 (Mouse); 83502 (Rat)
Alternate Names	Arc-1, CAD1, CAM 120/80, CD324, CD324 antigen, CDH1, CDHE, Cadherin-1, Cell-CAM120/80, E-Cadherin, ECAD, ECadherin, Epithelial cadherin, L-CAM, LCAM, UVOE-Cadherin, Uvomorulin, cadherin 1, E-cadherin (epithelial), cadherin 1, type 1, E-cadherin (epithelial), calcium-dependent adhesion protein, epithelial, cell-CAM 120/80

Citations for Human E-Cadherin Quantikine ELISA Kit (9)

Showing 1 - 9 of 9 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human E-Cadherin Quantikine ELISA Kit include:

Species: Human
Sample Types: Cell Culture Supernates

N Ali et al. (2020-06-29), Doublecortin-like kinase 1 promotes hepatocyte clonogenicity and oncogenic programming via non-canonical &beta-catenin-dependent mechanism Sci Rep, 2020-010(1):10578.
PMID: 32601309
Species: Human
Sample Types: Cell Culture Supernates

MJ Kim et al. (2019-10-22), Structure establishment of three-dimensional (3D) cell culture printing model for bladder cancer PLoS ONE, 2019-114(10):e0223689.
PMID: 31639124
Species: Human, Xenograft
Sample Types: Cell Culture Supernates

M Singh et al. (2019-04-05), Targeting the temporal dynamics of hypoxia-induced tumor-secreted factors halts tumor migration Cancer Res., 2019-00(0).
PMID: 30952634
Species: Human
Sample Types: Cell Culture Supernates

Dong et al. (2014-03-27), Meloxicam executes its antitumor effects against hepatocellular carcinoma in COX-2- dependent and -independent pathways. PLoS ONE, 2014-09(3):e92864.
PMID: 24675684
Species: Human
Sample Types: Cell Culture Supernates

Gaida M et al. (2012-11-02), Polymorphonuclear neutrophils promote dyshesion of tumor cells and elastase-mediated degradation of E-cadherin in pancreatic tumors. Eur J Immunol, 2012-142(12):3369-80.
PMID: 23001948
Species: Human
Sample Types: Serum

Zekri A et al. (2011-06-21), Serum levels of beta-catenin as a potential marker for genotype 4/hepatitis C-associated hepatocellular carcinoma. Oncol Rep, 2011-026(4):825-31.
PMID: 21701780
Species: Human
Sample Types: Plasma

Streeck H et al. (2011-03-14), Epithelial adhesion molecules can inhibit HIV-1-specific CD8+ T-cell functions. Blood, 2011-0117(19):5112-22.
PMID: 21403126
Species: Human
Sample Types: Plasma

Hung YJ et al. (2010-05-26), Plasma protein growth arrest-specific 6 levels are associated with altered glucose tolerance, inflammation, and endothelial dysfunction. Diabetes Care, 2010-033(8):1840-4.
PMID: 20504897
Species: Human
Sample Types: Serum

Jonsson MV et al. (2005-12-01), Elevated serum levels of soluble E-cadherin in patients with primary Sjogren's syndrome. Scand. J. Immunol., 2005-162(6):552-9.
PMID: 16316423

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