Assay Diluent RD1-63 contains visible precipitate. Is this acceptable to use?

Name: Human IFN-alpha 2 Quantikine HS ELISA Kit
Brand: R&D Systems
SKU: HSIFNA2

A small amount of precipitate is expected and acceptable in Assay Diluent RD1-63. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.

Human IFN-alpha 2 Quantikine HS ELISA Kit

Catalog # HSIFNA2 | R&D Systems, Inc. a Bio-Techne Brand

Product Datasheet / COA / SDS

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Key Product Details

Assay Length:	4.0 hours
Sample Type & Volume Required Per Well:	Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	0.107 pg/mL
Assay Range:	0.781 - 50 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Human IFN-alpha 2 Quantikine HS ELISA Kit Summary:	The Quantikine™ HS Human IFN-alpha 2 Immunoassay is a 4.0 hour solid phase ELISA designed to measure human IFN-alpha 2 (IFN-alpha 2a, IFN-alpha 2b, IFN-alpha 2c) in cell culture supernates, serum, and plasma. It contains HEK293-expressed recombinant human IFN-alpha 2a and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IFN-alpha 2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IFN-alpha 2.

View Full Technical Details for Human IFN-alpha 2 Quantikine HS ELISA Kit

Technical Data
Citations / Reviews / FAQs

Product Specifications for Human IFN-alpha 2 Quantikine HS ELISA Kit

Measurement	Quantitative ELISA
Detection Method	Colorimetric - 450 or 490nm (TMB or AP Substrate)
Conjugate	HRP
Reactivity	Human
Specificity	Natural and recombinant human IFN-alpha 2.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to asses inter-assay precision. Assays were performed by at least three technicians

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	2.38	15.1	32.3	2.65	15.5	29.7
Standard Deviation	0.082	0.686	1.36	0.15	0.965	1.69
CV%	3.4	4.5	4.2	5.7	6.2	5.7

Recovery

The recovery of human IFN-alpha 2 spiked to levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	101	90-108
EDTA Plasma (n=4)	89	75-101
Heparin Plasma (n=4)	86	75-92
Serum (n=4)	86	75-99

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human IFN-alpha 2 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Examples for Human IFN-alpha 2 Quantikine HS ELISA Kit

	Human IFN-Alpha 2 High Sensitivity Quantikine Kit Spiked Linearity IFN-Alpha 2 is spiked at high concentration in various matrices and diluted with appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. The linearity is between 87%-112% for Heparin Plasma, 97%-114% for EDTA Plasma, and 94%-111% for Serum.
	Human IFN-alpha 2 ELISA Standard Curve

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-alpha 2/IFNA2

Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.

Item	Value
Long Name	Interferon alpha 2
Entrez Gene IDs	3440 (Human); 15965 (Mouse); 298213 (Rat)
Alternate Names	IFN-alpha 2, IFN-alpha-2, IFN-alphaA, IFNA, IFNA2, IFNA2a, IFNA2b, IFNA2c, IFNAA, IFNalpha 2, INFA2, Ifa2, Interferon alpha-A, LeIF A, MGC125764, MGC125765, alpha-2a interferon, interferon alpha 2b, interferon alpha A, interferon alpha-2, interferon, alpha 2

Citations for Human IFN-alpha 2 Quantikine HS ELISA Kit

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FAQs for Human IFN-alpha 2 Quantikine HS ELISA Kit

Assay Diluent RD1-63 contains visible precipitate. Is this acceptable to use?

A small amount of precipitate is expected and acceptable in Assay Diluent RD1-63. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.