Human/Mouse ATG7 Antibody
Catalog # MAB6608 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Summary for Human/Mouse ATG7 Antibody
Immunogen
Asn387-Gln570
Accession # O95352
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human/Mouse ATG7 Antibody
Detection of Human ATG7 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ATG7 Monoclonal Antibody (Catalog # MAB6608) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ATG7 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.ATG7 in RAW 264.7 Mouse Cell Line.
ATG7 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Mouse Anti-Human ATG7 Monoclonal Antibody (Catalog # MAB6608) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to autophagosomes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.ATG7 in Human Brain.
ATG7 was detected in formalin fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human ATG7 Monoclonal Antibody (Catalog # MAB6608) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human/Mouse ATG7 Antibody
CyTOF-ready
Immunocytochemistry
Sample: Immersion fixed RAW 264.7 mouse monocyte/macrophage cell line
Immunohistochemistry
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)
Intracellular Staining by Flow Cytometry
Sample: HeLa human cervical epithelial carcinoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Simple Western
Sample: HeLa human cervical epithelial carcinoma cell line
Western Blot
Sample: HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line
Reviewed Applications
Read 3 reviews rated 5 using MAB6608 in the following applications:
Published Applications
Read 8 publications using MAB6608 in the following applications:
Formulation, Preparation and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ATG7
ATG7, also known as APG7, is a 78 kDa cytosolic ubiquitin-E1-like enzyme that plays a key role in the autophagic pathway of intracellular bulk degradation. It is required for the conjugation of ATG5 to ATG12, the lipidation of ATG8, and subsequent autophagosome formation. ATG7 is required for mitochondrial removal during erythropoiesis and for the maintenance of axonal homeostasis. Alternate splicing of human ATG7 generates an isoform that lacks 31 aa at the C-terminus, a region that is required for ATG8 lipidation. Human ATG7 shares 93% aa sequence identitiy with mouse and rat ATG7.
Product Documents for Human/Mouse ATG7 Antibody
Product Specific Notices for Human/Mouse ATG7 Antibody
For research use only
Citations for Human/Mouse ATG7 Antibody (8)
Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse ATG7 Antibody include:
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Species: Human
Sample Types: Cell Lysates
Applications: Western BlotD Beillevair et al. (2022-02-11), Autolysosomes and caspase-3 control the biogenesis and release of immunogenic apoptotic exosomes Cell Death & Disease, 2022-013(2):145.
PMID: 35149669 -
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western BlotK Pandey et al. (2020-06-17), Autophagy coupled to translation is required for long-term memory Autophagy, 2020-00(0).
PMID: 32501746 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotYH Sheng et al. (2020-06-01), A Nucleotide Analog Prevents Colitis-Associated Cancer via Beta-Catenin Independently of Inflammation and Autophagy Cell Mol Gastroenterol Hepatol, 2020-00(0).
PMID: 32497793 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotM Bernard et al. (2020-01-13), Autophagy drives fibroblast senescence through MTORC2 regulation Autophagy, 2020-00(0):1-13.
PMID: 31931659 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCS Jung et al. (2019-06-24), Autophagic death of neural stem cells mediates chronic stress-induced decline of adult hippocampal neurogenesis and cognitive deficits Autophagy, 2019-00(0):1-19.
PMID: 31234698 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryP Thapa et al. (2017-07-19), Histone deacetylase 3 is required for iNKT cell development Sci Rep, 2017-07(1):5784.
PMID: 28724935 -
Species: Human
Sample Types: Tissue Homogenates
Applications: Western BlotMaurizio Bossola (2016-11-12), Altered mitochondrial quality control signaling in muscle of old gastric cancer patients with cachexia Exp. Gerontol., 2016-10(0).
PMID: 27847330 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotBernard M et al. (2014-01-01), Autophagy fosters myofibroblast differentiation through MTORC2 activation and downstream upregulation of CTGF. Autophagy, 2014-010(12):2193-207.
PMID: 25495560
There are no citations that match your criteria.
Customer Reviews for Human/Mouse ATG7 Antibody (3)
Customer Images
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Application: ImmunohistochemistrySample Tested: Brain tissueSpecies: MouseVerified Customer | Posted 03/23/2022
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Application: ImmunohistochemistrySample Tested: Ocular melanomaSpecies: HumanVerified Customer | Posted 09/07/2021
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Application: Western BlotSample Tested: Human fibroblast cell lineSpecies: HumanVerified Customer | Posted 07/12/2017for WB: blocking 1 h 5% skimmed milk in TBST; Ab o/n @ 4 °C 1:1000 in TBST. Beclin1-specific siRNA-transfected cells do not show a signal.
There are no reviews that match your criteria.
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