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Human/Mouse/Rat SOX2 Antibody Best Seller

Catalog # MAB2018 | R&D Systems, Inc. a Bio-Techne Brand
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MAB2018
MAB2018-SP

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-reported, Immunocytochemistry, Immunohistochemistry, Intracellular Staining by Flow Cytometry, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 245610

Product Summary for Human/Mouse/Rat SOX2 Antibody

Immunogen

E. coli-derived recombinant human SOX2
Gly135-Met317
Accession # P48431

Specificity

Detects human, mouse, and rat SOX2 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human/Mouse/Rat SOX2 Antibody

E-Cadherin and SOX2 antibody in BG01V Human Stem Cells by Immunocytochemistry (ICC).

E‑Cadherin and SOX2 in BG01V Human Stem Cells.

E-Cadherin and SOX2 were detected in BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human E-Cadherin Antigen Affinity-purified Polyclonal Antibody (AF648) and 10 µg/mL Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for E-Cadherin using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and for SOX2 using the NorthernLights 493-conjugated Anti-Mouse Secondary Antibody (green; NL009). Cells were counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human, Mouse, and Rat SOX2 antibody by Western Blot.

Detection of Human, Mouse, and Rat SOX2 by Western Blot.

Western blot shows lysates of NTera-2 human testicular embryonic carcinoma cell line, F9 mouse teratocarcinoma stem cells, D3 mouse embryonic stem cell line, and rat cortical stem cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for SOX2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
SOX2 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC).

SOX2 in BG01V Human Embryonic Stem Cells.

SOX2 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human/ Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Mouse/Rat SOX2 Antibody

Application
Recommended Usage

CyTOF-reported

Zunder, E.R. et al. (2015) Cell Stem Cell 16: 323. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells and immersion fixed NTera‑2 human embryonic carcinoma cells

Immunohistochemistry

3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of glioblastoma

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: NTera‑2 human embryonic carcinoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Simple Western

4 µg/mL
Sample: BG01V human embryonic stem cells

Western Blot

1 µg/mL
Sample: NTera‑2 human testicular embryonic carcinoma cell line, F9 mouse teratocarcinoma stem cells, D3 mouse embryonic stem cell line, and rat cortical stem cells
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 13 reviews rated 4.5 using MAB2018 in the following applications:

Published Applications

Read 183 publications using MAB2018 in the following applications:

Formulation, Preparation and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOX2

SOX2 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX2 functions in specifying the first three lineages present at implantation and in regulating proliferation and differentiation in the developing peripheral nervous system (1-6).

References

  1. Graham, V. et al. (2003) Neuron 39:749.
  2. Avilion, A.A. et al. (2003) Genes Dev. 17:126.
  3. Kishi, M. et al. (2000) Development 127:791.
  4. Yuan, H. et al. (1995) Genes Dev. 9:2635.
  5. Uwanogho, D. et al. (1995) Mech. Dev. 49:23.
  6. Stevanovic, M. (2003) Mol. Biol. Rep. 30:127.

Long Name

Transcription Factor SOX2

Alternate Names

SOX2, ANOP3, MCOPS3, MGC2413, SRY (sex determining region Y)-box 2, SRY-related HMG-box gene 2, transcription factor SOX-2, transcription factor SOX2

Entrez Gene IDs

6657 (Human)

Gene Symbol

SOX2

UniProt

Product Documents for Human/Mouse/Rat SOX2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse/Rat SOX2 Antibody

For research use only

Citations for Human/Mouse/Rat SOX2 Antibody (183)

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Customer Reviews for Human/Mouse/Rat SOX2 Antibody (13)

4.5 out of 5
13 customer ratings
5 stars
46%
4 stars
54%
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Showing  1 - 5 of 13 reviews Showing All
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  • Human/Mouse/Rat SOX2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: glioblastoma cells
    Species: Human
    Verified Customer | Posted 08/06/2021
    On the photo SOX2 is red, nuclear DAPI is blue.
    Human/Mouse/Rat SOX2 Antibody MAB2018
  • Human/Mouse/Rat SOX2 Antibody
    Name: Shuba Krishnan
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: iPS2 human induced pluripotent stem cells
    Species: Human
    Verified Customer | Posted 10/18/2018
    Cells: human induced pluripotent stem cells Primary antibody: anti-human SOX2 (1:200), O/N incubation at 4 degrees Secondary antibody: anti-mouse AF488
    Human/Mouse/Rat SOX2 Antibody MAB2018
  • Human/Mouse/Rat SOX2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Brain (forebrain)
    Species: Mouse
    Verified Customer | Posted 02/16/2017
    Red: sox2. Green: ki67. Blue: DAPI. Postnatal day 7 sub-ventricular zone. 2% PFA fixed. Heat-mediated antigen retrieval. Blocked with 10% donkey serum, 0.5% Triton in PBS for 1h. Dilution 1:100.
    Human/Mouse/Rat SOX2 Antibody MAB2018
  • Human/Mouse/Rat SOX2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Neural progenitor cells
    Species: Human
    Verified Customer | Posted 02/03/2017
  • Human/Mouse SOX2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Induced pluripotent stem cells
    Species: Human
    Verified Customer | Posted 12/15/2016
    I used this antibody to test Sox2 expression in iPS cells derived from fibroblasts of Sanfilippo syndrome C patients.
  • Human/Mouse SOX2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: human NSC
    Species: Human
    Verified Customer | Posted 10/26/2015
    Specificity: Specific
    Sensitivity: Reasonably sensitive
    Buffer: 1% BSA + 0.3% Triton X-100 in PBS
    Dilution: 1/100
    Human/Mouse/Rat SOX2 Antibody MAB2018
  • Human/Mouse SOX2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: human fibroblast and neural stem cells
    Species: Human
    Verified Customer | Posted 10/26/2015
    Specificity: Specific
    Sensitivity: Reasonably sensitive
    Buffer: 1% BSA + 0.3% Triton X-100 in PBS
    Dilution: 1/100
    Human/Mouse/Rat SOX2 Antibody MAB2018
  • Human/Mouse SOX2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Human cells
    Species: Human
    Verified Customer | Posted 10/26/2015
    Specificity: Specific
    Sensitivity: Sensitive
    Buffer: 5% BSA in TBST
    Dilution: 1/1000
    Human/Mouse/Rat SOX2 Antibody MAB2018
  • Name: cynthia morimoto
    Application: Immunofluorescence
    Sample Tested: See PMID 22791629
    Species: Rat
    Verified Customer | Posted 02/16/2015
  • Name: cynthia morimoto
    Application: Immunofluorescence
    Sample Tested: See PMID 19824823
    Species: Human
    Verified Customer | Posted 02/16/2015
  • Name: cynthia morimoto
    Application: Immunofluorescence
    Sample Tested: See PMID 19825428
    Species: Mouse
    Verified Customer | Posted 02/16/2015
  • Name: cynthia morimoto
    Application: Immunofluorescence
    Sample Tested: See PMID 23658023
    Species: Human
    Verified Customer | Posted 02/16/2015
  • Name: cynthia morimoto
    Application: Immunofluorescence
    Sample Tested: See PMID 22573702
    Species: Other
    Verified Customer | Posted 02/16/2015

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FAQs for Human/Mouse/Rat SOX2 Antibody

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  • Q: What is the light chain of Human/Mouse/Rat SOX2 Antibody, Catalog #s MAB2018, MAB2018R and IC2018?

    A: Catalog #s MAB2018, MAB2018R and IC2018 all have a kappa light chain.

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