Human p63/TP73L Antibody

Catalog # AF1916 | R&D Systems, Inc. a Bio-Techne Brand
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AF1916
AF1916-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human p63/TP73L Antibody

Immunogen

E. coli-derived recombinant human p63/TP73L
Met40-Cys339
Accession # Q9H3D4

Specificity

Detects human p63/TP73L in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human p63/TP73L Antibody

p63/TP73L antibody in Human Breast by Immunohistochemistry (IHC-P).

p63/TP73L in Human Breast.

p63/TP73L was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human p63/TP73L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1916) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
p63/TP73L antibody in SCC-25 Human Cell Line by Immunocytochemistry (ICC).

p63/TP73L in SCC-25 Human Cell Line.

p63/TP73L was detected in immersion fixed SCC-25 human tongue carcinoma cell line using Goat Anti-Human p63/TP73L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1916) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human p63/TP73L Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed FaDu human hypopharyngeal carcinoma cell line and SCC-25 human tongue carcinoma cell line

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast

Western Blot

0.1 µg/mL
Sample: Recombinant Human p63/TP73L
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 4.3 using AF1916 in the following applications:

Published Applications

Read 8 publications using AF1916 in the following applications:

Formulation, Preparation and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p63/TP73L

Tumor Protein 63 (p63), also named TP73L, TP63, p51, p40 or KET, is a p53 homolog. It is one of several proteins that are produced from a single gene using two promoters and alternative splicing of the primary RNA transcript. p63 is highly expressed in embryonic ectoderm and in the nuclei of basal regenerative cells of many epithelial tissues in the adult. p63 is suggested to play a role in development, epithelial cell maintenance and tumorigenesis (1‑3).

References

  1. Harms, K. et al. (2004), Cell Mol. Life Sci. 61(7):822.
  2. Koster, M.I. and D.R. Roop, J. Dermatol. Sci. 34(1):3.
  3. Benard, J. et al. (2003) Hum. Mutat. 21(3):182.

Long Name

Tumor Protein 63

Alternate Names

EEC3, KET, OFC8, p40, p51, p73L, SHFM4, TP63, TP73L

Entrez Gene IDs

8626 (Human)

Gene Symbol

TP63

UniProt

Q9H3D4

Product Documents for Human p63/TP73L Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human p63/TP73L Antibody

For research use only

Citations for Human p63/TP73L Antibody (8)

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Customer Reviews for Human p63/TP73L Antibody (4)

4.3 out of 5
4 customer ratings
5 stars
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  • Human p63/TP73L Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: mouse lung tissue and Mouse lung paraffin embedded sections
    Species: Mouse
    Verified Customer | Posted 09/23/2020
    p63/TP73L was detected in paraffin-embedded mouse tissue from Lkb1fl/fl; Ptenfl/fl and Sox2LSL/LSL ;Nkx2-1fl/fl; Lkb1fl/fl (SNL) mice, which present with lung squamous cell carcinoma (LSCC) tumors, using the Goat Anti-Human p63/TP73L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1916) overnight at 4℃ in blocking buffer at a dilution of 1/400. Citrate buffer pH 6.0 was used for high temperature antigen retrieval for 15 minutes in a pressure cooker. Tissue was stained using a HRP-conjugated secondary antibody and DAB staining and counterstained with hematoxylin (blue). P63 positivity was seen in the basal cells found in the trachea of mice and in LSCC tumors but not in small cell lung cancer (SCLC) tumors from Rb1/p53/Myc and Rb1/p53/Rbl2 mice or in mucinous adenocarcinoma tumors found in SNL mice.
    Human p63/TP73L Antibody AF1916
  • Human p63/TP73L Antibody
    Name: Michele Torino
    Application: Western Blot
    Sample Tested: 293T human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 12/07/2017
  • Human p63/TP73L Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Mammary gland tissue
    Species: Human
    Verified Customer | Posted 05/16/2017
  • Human p63/TP73L Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Prostate cancer
    Species: Human
    Verified Customer | Posted 12/15/2016
    p63 is prostate stem cell marker. i have isolated stem cells from BPH patients and stained with p63 marker. I found excellent results with DAB kit and also p63 antibody performed superb job.

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