ITS Media Supplement (100X) Best Seller

Name: ITS Media Supplement (100X)
Brand: R&D Systems
SKU: AR013
Rating: 5 (5 reviews)

Catalog # AR013 | R&D Systems, Inc. a Bio-Techne Brand

(5)

Citations (1)

Product Datasheet / COA / SDS

Insulin-Transferrin-Selenium Media Supplement

Key Product Details

Setting the standard in quality research reagents

Summary for ITS Media Supplement (100X)

Kit Summary

Supports cell proliferation under low serum conditions.

Key Benefits

Contains optimized concentrations of insulin, transferrin, and selenite
Chemically-defined media decreases experimental variation
High lot-to-lot consistency decreases variation
Performance tested on multiple cell lines

Why Use Pre-optimized and Serum-free Supplements for Cell Culture?

The efficiency of cell proliferation and maintenance of cell health can vary from one experiment to another depending on the media components, the supplements used to drive proliferation, and the quality of the starting cell population.

Although commonly used, serum-containing medium is inherently variable due to the undefined composition and lot-to-lot variability across batches of serum. Troubleshooting media variability can be a costly and time-consuming obstacle that can be minimized through the use of high quality media. Our ITS Media Supplement is a pre-optimized and serum-free media supplement that was designed to help maintain culture media consistency across passages and experiments. The ITS Media Supplement contains optimized concentrations of human insulin, human transferrin, and selenite.

ITS Media Supplement (100x):

Contains optimized concentrations of insulin, transferrin, and selenite
Chemically-defined media decreases experimental variation
High lot-to-lot consistency decreases variation
QC-tested on multiple cell lines

Kit Contents

5 mL ITS Media Supplement (100x)

Component	Amount (µg/mL)
Recombinant Human Insulin	1000
Human Transferrin	500
Selenite	0.67

Stability and Storage

Store in the dark at °C in a manual defrost freezer. Do not use past the expiration date.

Precautions

This product contains human transferrin. This transferrin was tested at the donor level using an FDA licensed method and found to be non-reactive for anti-HIV-1/2 and Hepatitis B surface antigen. As no testing can offer complete assurance of freedom from infectious reagents, these reagents should be handled as if capable of transmitting infection.

Limitations

FOR LABORATORY RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
The safety and efficacy of this product in diagnostic or other clinical uses has not been established.
This reagent should not be used beyond the expiration date indicated on the label.

Product Specifications for ITS Media Supplement (100X)

Species

Multi-Species

Scientific Data Examples for ITS Media Supplement (100X)

	ITS Media Supplement Supports Proliferation Under Low Serum Conditions. ITS Media Supplement Supports Proliferation Under Low Serum Conditions.Hela, MCF7, and HepG2 cells were cultured in media supplemented with 10% FBS, low serum (0.1% FBS), or ITS Media Supplement + 0.1% FBS. Cells were trypsinized, harvested, and cell counts were performed. For each respective cell line, cell counts were normalized against standard 10% FBS-containing media (100%).

Preparation & Storage

Shipping Conditions	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Storage	Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Assay Procedure

Refer to the product datasheet for complete product details.

Briefly, ITS Media Supplement is prepared using the following procedure:

Dilute the media into the cell culture media of interest
Store completed media at 2 – 8 °C

Reagents Provided

Reagents provided in the ITS Media Supplement (100X) (Catalog # AR013):

Component	Amount (µg/mL)
Recombinant Human Insulin	1000
Human Transferrin	500
Selenite	0.67

Stability and Storage

Store in the dark at < -20 ^°C in a manual defrost freezer. Do not use past the expiration date.

Precautions

Other Supplies Required

Materials

Basal media
Serological pipettes
Pipettes and pipette tips

Equipment

2 °C to 8 °C refrigerator

Procedure Overview

Dilute 100-fold into cell culture media of interest before use. The final concentration of serum required with ITS supplementation is cell type and assay dependent. Titrate for individual use.

Citations for ITS Media Supplement (100X) (1)

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Citations are publications that use Bio-Techne products. Selected citations for ITS Media Supplement (100X) include:

Species: Human
Sample Types: Whole Cells

V Miceli et al. (2019-02-18), Comparison of Immunosuppressive and Angiogenic Properties of Human Amnion-Derived Mesenchymal Stem Cells between 2D and 3D Culture Systems Stem Cells Int, 2019-02019(0):7486279.
PMID: 30911299

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Customer Reviews for ITS Media Supplement (100X) (5)

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Product Documents for ITS Media Supplement (100X)

Product Datasheets

Download Product Datasheets

COA

SDS

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ITS Media Supplement (100X) Best Seller

Catalog # AR013 | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Summary for ITS Media Supplement (100X)

Key Benefits

Why Use Pre-optimized and Serum-free Supplements for Cell Culture?

ITS Media Supplement (100x):

Stability and Storage

Precautions

Limitations

Product Specifications for ITS Media Supplement (100X)

Scientific Data Examples for ITS Media Supplement (100X)

Preparation & Storage

Assay Procedure

Reagents provided in the ITS Media Supplement (100X) (Catalog # AR013):

Stability and Storage

Precautions

Materials

Equipment

Citations for ITS Media Supplement (100X) (1)

Customer Reviews for ITS Media Supplement (100X) (5)

ITS Media Supplement (100X)

ITS Media Supplement (100X)

ITS Media Supplement (100X)

ITS Media Supplement (100X)

ITS Media Supplement (100X)

Product Documents for ITS Media Supplement (100X)

Product Datasheets

COA

Certificate of Analysis

SDS

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