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Janelia Fluor® 669, SE
Catalog # 6420 | Tocris Bioscience a Bio-Techne Brand
Far red dye (Reactive group: NHS ester). Applications: super resolution microscopy e.g. dSTORM & STED, confocal and live cell imaging
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Key Product Details
| Description: | Far red dye (Reactive group: NHS ester). Applications: super resolution microscopy e.g. dSTORM & STED, confocal and live cell imaging |
| Alternative Names: | JF 669, NHS |
| Chemical Name: | 1-[7-(1-Azetidinyl)-10-[2-Carboxy-5-[(2-(2,5-dioxopyrrolidin-1-yl)oxy)-2-oxoethyl]thio-3,4,6-trifluorophenyl]-9,9-dimethyl-9-silaanthracen-2(9H)-ylidene]azetidinium, inner salt |
| Purity: | ≥90% (HPLC) |
| Molecular Weight: | 693.77 |
Biological Activity for Janelia Fluor® 669, SE
Janelia Fluor® 669, SE is a red fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells). Cell permeable. Excitation maximum = 669 nm; emission maximum = 682 nm; Quantum yield = 0.37; Extinction coefficient = 116,000 M-1cm-1; Correction factor = 0.0430. Retains 97% fluorescence after 30 bleaching cycles.
Please see the protocol for further information on protein/antibody labeling and conjugation.
We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.
HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Janelia Fluor® 669, SE
| Emission Color | Far Red |
|---|---|
| Brightness | |
| λabs | 669 nm |
| λem | 682 nm |
| Extinction Coefficient (ε) | 116,000 M-1cm-1 |
| Quantum Yield (φ) | 0.37 |
| Closest Laser line | 640 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | Yes |
| Correction Factor 280 | 0.043 |
| Application | Confocal Microscopy, Super Resolution Microscopy (SRM) |
| Alternative For | Alexa Fluor® 660, Atto 655, Atto 665, CF660R |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
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Applications on Tubulin for Janelia Fluor® 669, SE. Application of Janelia Fluor® 669, SE dye on Tubulin; Left: Microtubules were labeled with anti-alpha -tubulin primary antibodies. The secondary antibodies were conjugated to Janelia Fluor® 669, SE. Maximum intensity projection of confocal z-stack, recorded with an Olympus FV1200 confocal microscope, using 638 nm laser line for JF 669. Right: Enlarged portion of left-hand image. Images kindly provided by Dr Kirstin Elgass, Monash University. |
Plan Your Experiments
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Citations for Janelia Fluor® 669, SE (1)
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Citations are publications that use Bio-Techne products. Selected citations for Janelia Fluor® 669, SE include:
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Hui et al.
(2021)
EASI-FISH for thick tissue defines lateral hypothalamus spatio-molecular organization.
Cell.
184:6361-6377.e24
PMID: 34875226
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