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RNAscope ISH Technology Automated Assays - Leica
The RNAscope in situ Hybridization Technology has been developed for both manual and automated protocols. If you require high throughput or an automated solution, ACD has partnered with Leica Biosystems to deliver RNAscope ISH automated assays.
RNAscope 2.5 Assays, available in Brown, Red and Multiplex, RNAscope 2.5 LS Reagent Kits, RNAscope LS Duplex Reagent Kits, BaseScope and miRNAscope Assays have been developed for the Leica automated IHC ISH Platform.
RNAscope 2.5 Leica Assay - BROWN
RNAscope 2.5 Leica Assay - BROWN
Designed to provide more intense Diaminobenzidine (DAB) staining.
Ideal for low copy target gene expression (1-20 copies per cell).
Visualized with bright-field microscopy.
RNAscope 2.5 Leica Assay - RED
RNAscope 2.5 Leica Assay - RED
Offers high contrast with Fast Red dye.
Red dots are clearly distinguished against the hematoxylin staining.
Visualized with standard bright-field microscopy.
BaseScope Leica Assay - RED
BaseScope Leica Assay - RED
Detection of short RNA targets and exon junctions.
High contrast Fast Red dye, distinct against hematoxylin staining.
Visualized with bright-field microscopy.
miRNAscope Leica Assay - RED
miRNAscope Leica Assay - RED
Detects small RNAs including the detection ASOs, miRNAs, siRNSa, and smaller RNAs (17-50 bases).
miRNAscope LS probes are not compatible with RNAscope LS or BaseScope LS reagents.
RNAscope Leica Multiplex Fluorescent Assay
RNAscope Leica Multiplex Fluorescent Assay
Simultaneous detection of up to four RNA targets, with up to four target probes.
Four target probes, each designed a different probe channel - C1, C2, C3 or C4.
Nuclei can be counter stained and visualized using DAPI fluorescent dye.
RNAscope 2.5 Leica Duplex Assay
RNAscope 2.5 Leica Duplex Assay
Designed for simultaneous in situ detection of two RNA species.
Two targets are distinguished in probe Channels 1 (C1, DAB) and Channel 2 (C2, Fast Red).
Visualized with a bright-field microscopy.
RNAscope 2.5 LSx Reagent Kit - BROWN
RNAscope 2.5 LSx Reagent Kit - BROWN
Pre-filled to accommodate the processing of 60 slides.
Reagents come pre-filled in Bond Rx containers fixed on two trays; include Fast Red chromogen, hematoxylin, bluing and proteases.
Results are comparable to the existing RNAscope 2.5 LS assay.
RNAscope 2.5 LSx Reagent Kit - RED
RNAscope 2.5 LSx Reagent Kit - RED
Pre-filled to accommodate the processing of 60 slides.
Reagents come pre-filled in Bond Rx containers fixed on two trays; include Fast Red chromogen, hematoxylin, bluing and proteases.
Results are comparable to the existing RNAscope 2.5 LS assay.
Leica Automated Assays
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Automated System |
Leica BOND RX System |
Leica BOND RX System |
Leica BOND RX System |
Leica BOND RX System |
Leica BOND RX System |
Leica BOND RX System |
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Assay Type |
Chromogenic |
Chromogenic |
Chromogenic |
Fluorescent |
Chromogenic |
Chromogenic |
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Dye Used |
Diaminobenzidine (DAB) |
Fast Red |
DAB & Fast Red Option for Green & Fast Red |
TSA-based Vivid / Opal fluorophores |
Fast Red |
Fast Red |
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Multiplexing |
Singleplex |
Singleplex |
Singleplex, Duplex |
Single to Fourplex |
Singleplex |
Singleplex |
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Key Benefit |
Robust, sensitive, permanent stain Most widely used RNAscope assay |
Provides bright color stains with high contrast to background |
Simultaneous in situ detection of two RNA species |
Utilizes up to 4 different spectral channels providing high flexibility |
Specific detection of splice variants/exon junctions and short targets |
Robust and specific detection of small RNAs |
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Ideal for |
High throughput Routine applications |
Studies of tissues with endogenous color background such as melanin in skin, liver, or lung from smokers |
Co-localization studies to map co-expression of two targets (e.g., secreted ligand and its receptor) |
Co-localization studies of up to 4 gene simultaneously Experimental application requiring flexibility |
Detection of splice variants and exon junctions, short insertions/deletions, short targets of complex & highly homologous gene families |
Detection of small RNAs including ASOs, miRNAs, piRNAs, shRNAs, siRNAs, and tRNAs |
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Novel Gene or Unknown Expression |
Compatible |
Compatible |
Compatible |
Compatible |
Compatible |
Compatible |
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Archival Specimens |
Compatible |
Compatible |
Compatible |
Compatible |
Compatible |
Compatible |
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Microscope Imaging System |
Standard bright field |
Standard bright field |
Standard bright field |
Multispectral fluorescent imaging |
Standard bright field |
Standard bright field |
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Sample Type |
FFPE |
FFPE |
FFPE |
FFPE Tissues (TMAs) |
FFPE |
FFPE |
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Quantitation Method |
Visual inspection |
Visual inspection |
Visual inspection |
Visual inspection |
Visual inspection |
Visual inspection |
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# of tissue sections per kit |
60 (20 mm x 20 mm) |
60 (20 mm x 20 mm) |
60 (20 mm x 20 mm) |
60 (20 mm x 20 mm) |
60 (20 mm x 20 mm) |
60 (20 mm x 20 mm) |
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Target Sequence length required |
300-1000bp |
300-1000bp |
300-1000bp |
300-1000bp |
50-300bp |
17-50bp |
