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Shining a Light on Limited and Complex Sample Types

Limited and Complex Sample Analysis with Simple Western

Whether you’re working with brain tissue homogenates, exosomes, or non-proliferating cells, our Simple Western™ systems maximize protein characterization data to shine light on limited and complex samples.

• Up to 8 protein targets with <5 µL of sample           • Up to 96 samples in a single hands-free run
• Results ready in as few as 3 hours • Analytical-grade quantification without matrix effects
Blue Green - Light Brain Icon

Quantify protein biomarkers in complex samples like brain tissue homogenates without matrix effects.

Blue Green - Light Exosome Icon

Optimize your exosome and extracellular vesicle (EV) analysis workflow using off-the-shelf antibodies.

Blue Green - Light Virus Icon

Monitor protein expression potency and other CQAs of viral vectors without purifying your samples.

Blue Green - Light Monocytes-Macrophages Icon

Unlock the potential of non-proliferating cells like induced pluripotent stem cell (iPSC)-derived macrophages.

Blue Green - Light Stem cells Icon

Unravel signal pathways during stem cell differentiation with flexible high-sensitivity multiplexing strategies.

Blue Green - Light Magnifying glass Icon

Overcome challenges with laser-capture microdissection (LCM) and formalin-fixed paraffin-embedded (FFPE) tissue analysis.

Cell and Tissue Lysates

Remove Matrix Effects, Simplify Assay Development, and Get Protein Separation Profiles

Matrix effects can interfere with your ELISA and flow cytometry experiments. Learn how Simple Western overcomes matrix effects of complex samples like cell lysates and tissue homogenates with improved specificity due to protein separation profiles. Unlike custom sandwich ELISA, Simple Western simplifies assay development using only one target-validated antibody.

  ELISA Simple Western
Sample volume:     50 µL      3 µL
Liquid waste: Yes No
Size separation: No Yes
Matrix effects: Yes No

Exosomes and Extracellular Vesicles (EV)

Fast, Validated EV Protein Analysis Workflow

An easy workflow for EV protein analysis using a simple column and hands-free, automated Western blotting analysis. Learn more.

  • Standardized exosome isolation and protein analysis
  • Low sample volume requirements which are ideal for your QC workflows
  • High sensitivity detection of low abundance EV proteins with conventional antibodies
  • Acceleration of exosome biomarker discovery studies, exosome research and liquid biopsy analysis
  • View our exosome characterization Protocol
Adeno Associated Virus

Viral Vectors

Protein Expression Potency Directly in Cell Lysates

  • Relative protein expression potency in cell lysates with high specificity and <5% CVs
  • Absolute protein expression measurements in cell lysates using a recombinant standard curve
  • Detection of residual intracellular capsid proteins in cell lysates as an infectivity surrogate
  • Download our protein expression potency App Note

Plus, Simple Western is a multi-attribute method beyond protein expression potency assays for quantitative CQA monitoring of purity, titer, empty/ full capsid content, and capsid protein ratio.

Non-Proliferating Cells

Cells Don’t Divide? No Problem.

"I used Simple Western because the protein samples I had were small. We only had a small number of [iPSC-derived macrophages] because the cell line does not proliferate." 

– Dr. Elena Navarro-Guerrero, Senior Postdoctoral Researcher, Nuffield Department of Medicine, Target Discovery Institute, Oxford University

Elena Navarro-Guerrero
The automated high-throughput Simple Western assay can analyze up to 96 samples overnight, enabling the analysis of full signaling pathways in one run.

Full signal pathway analysis in a single overnight hands-free run

Signal Pathway Analysis

Industry-Leading Detection Sensitivity and Multiplex Flexibility

High-sensitivity multiplex assays with Stellar NIR/IR Modules for Jess™ reveal and quantify changes in the expression of pluripotent factors and cell signaling proteins that regulate stem cell differentiation. 

Take the Complexity Out of Cell Signaling

Have you ever wanted to analyze a full signaling cascade in each of your samples in a single experiment? With Simple Western, each 5-μL sample may be interrogated up to 8 times to detect up to 8 distinct protein targets.

Simple Western Size on Wes detects myosin heavy chain 7 (MHC7) with 1, 2 and 3 μL of LCM sample

Simple Western analysis of LCM samples (1-3 µL)

FFPE and LCM Samples

The Key to Unlock Your Formalin-Fixed, Paraffin-Embedded (FFPE) Tissue Archive

For over a century, huge repositories of FFPE samples have been collected worldwide at universities and hospitals, creating a rich and largely untapped resource to study cancer and other diseases. A major advantage of the Simple Western Total Protein Assay for FFPE applications is that it bypasses interference of the reducing agent β-mercaptoethanol (β-ME) that is present in processed FFPE extracts.

Don’t Waste Precious Laser-Capture Microdissection (LCM) Samples

With Simple Western, you only need a few microliters of LCM sample for each data point, so you’ll get more data points for each sample you collect.

Additional Resources

Customer Success Stories