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Shining a Light on Limited and Complex Sample Types
Limited and Complex Sample Analysis with Simple Western
Whether you’re working with brain tissue homogenates, exosomes, or non-proliferating cells, our Simple Western™ systems maximize protein characterization data to shine light on limited and complex samples.
| • Up to 8 protein targets with <5 µL of sample | • Up to 96 samples in a single hands-free run |
| • Results ready in as few as 3 hours | • Analytical-grade quantification without matrix effects |
Cell and Tissue Lysates
Remove Matrix Effects, Simplify Assay Development, and Get Protein Separation Profiles
Matrix effects can interfere with your ELISA and flow cytometry experiments. Learn how Simple Western overcomes matrix effects of complex samples like cell lysates and tissue homogenates with improved specificity due to protein separation profiles. Unlike custom sandwich ELISA, Simple Western simplifies assay development using only one target-validated antibody.
| ELISA | Simple Western | |
| Sample volume: | 50 µL | 3 µL |
| Liquid waste: | Yes | No |
| Size separation: | No | Yes |
| Matrix effects: | Yes | No |
Exosomes and Extracellular Vesicles (EV)
Fast, Validated EV Protein Analysis Workflow
An easy workflow for EV protein analysis using a simple column and hands-free, automated Western blotting analysis. Learn more.
- Standardized exosome isolation and protein analysis
- Low sample volume requirements which are ideal for your QC workflows
- High sensitivity detection of low abundance EV proteins with conventional antibodies
- Acceleration of exosome biomarker discovery studies, exosome research and liquid biopsy analysis
- View our exosome characterization Protocol
Viral Vectors
Protein Expression Potency Directly in Cell Lysates
- Relative protein expression potency in cell lysates with high specificity and <5% CVs
- Absolute protein expression measurements in cell lysates using a recombinant standard curve
- Detection of residual intracellular capsid proteins in cell lysates as an infectivity surrogate
- Download our protein expression potency App Note
Plus, Simple Western is a multi-attribute method beyond protein expression potency assays for quantitative CQA monitoring of purity, titer, empty/ full capsid content, and capsid protein ratio.
Non-Proliferating Cells
Cells Don’t Divide? No Problem.
"I used Simple Western because the protein samples I had were small. We only had a small number of [iPSC-derived macrophages] because the cell line does not proliferate."
– Dr. Elena Navarro-Guerrero, Senior Postdoctoral Researcher, Nuffield Department of Medicine, Target Discovery Institute, Oxford University
Full signal pathway analysis in a single overnight hands-free run
Signal Pathway Analysis
Industry-Leading Detection Sensitivity and Multiplex Flexibility
High-sensitivity multiplex assays with Stellar NIR/IR Modules for Jess™ reveal and quantify changes in the expression of pluripotent factors and cell signaling proteins that regulate stem cell differentiation.
Take the Complexity Out of Cell Signaling
Have you ever wanted to analyze a full signaling cascade in each of your samples in a single experiment? With Simple Western, each 5-μL sample may be interrogated up to 8 times to detect up to 8 distinct protein targets.
Simple Western analysis of LCM samples (1-3 µL)
FFPE and LCM Samples
The Key to Unlock Your Formalin-Fixed, Paraffin-Embedded (FFPE) Tissue Archive
For over a century, huge repositories of FFPE samples have been collected worldwide at universities and hospitals, creating a rich and largely untapped resource to study cancer and other diseases. A major advantage of the Simple Western Total Protein Assay for FFPE applications is that it bypasses interference of the reducing agent β-mercaptoethanol (β-ME) that is present in processed FFPE extracts.
Don’t Waste Precious Laser-Capture Microdissection (LCM) Samples
With Simple Western, you only need a few microliters of LCM sample for each data point, so you’ll get more data points for each sample you collect.
Additional Resources
- Monitor your mAb with selectivity and specificity for upstream bioprocessing
- Characterize phospho-protein isoforms in complex organoids
- Quantitative profiles of MAPK and PI3K proteins in clinical specimens
- Get more data out of precious samples
- Webinar: Tackling the challenges of human disease and vaccine research
Customer Success Stories
Postdoctoral Fellow, UT Health San Antonio
Senior Lecturer in Drug Action, University of Manchester
Director of Research Operations/Scientist, Roseman Univ.
Postdoctoral Researcher, Medical University of Vienna
