MauriceFlex: Flex Your Freedom

Flex Your Freedom

Introducing MauriceFlex, your new protein fractionation solution that lets you collect fractions for charge variant analysis. MauriceFlex gives you:

  • Freedom to do more with your fractions – analyze with intact mass, reduced and subunit mass, peptide mapping, and more
  • Freedom from laborious IEX and HPLC workflows – easily collect high-purity fractions without separate extensive development of chromatography-based fractionation
  • Freedom to do it all - icIEF, CE-SDS, and icIEF protein fractionation all in one instrument
  • Freedom to do more with your time - identify, separate, and fractionate charge variants in less than a day
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Table of Contents

 

MauriceFlex combines protein charge isoform fractionation with imaged-capillary isoelectric focusing (icIEF) and CE-SDS analysis in one instrument, so that you can go beyond routine size and charge and conduct charge variant analysis of your molecule at any stage without needing to rely on different methods like ion-exchange chromatography (IEX) and HPLC.

Are You Using IEX or HPLC for Protein Fractionation?

Protein fractionation with MauriceFlex renders significant advantages on time and capital when compared with IEX, HPLC and other existing technologies. The key advantages are:

  • Same-day fraction collection
  • High purity fractions
  • Single fractionation offering enough material for intact MS analysis
  • Compatibility with urea and methylcellulose
  • Ability to pool fractions for peptide mapping
  • Same system offering CE-SDS and cIEF assays
  • Eliminating the need for bridging studies
MauriceFlex Instrument performs ic-ief based protein fractionation

Featured Resources

MauriceFlex Spotlight Highlighting ICIEF fractionation workflow for antibody standard

Application Spotlight

Explore how NIST mAb samples were fractionated on MauriceFlex and further analyzed with LC-MS for intact mass and peptide mapping.

Thumbnail image of webinar - charged protein

Webinar

Hear about unraveling the charge heterogeneity of biotherapeutics with MauriceFlex™ 

c-ief protein fractionation with MauriceFlex Poster Teaser Card

Scientific Poster

Learn about novel icIEF Fractionation Coupled with Different MS Systems for Rapid Charge Variant Analysis of Therapeutic Antibodies

icIEF Protein Fractionation Workflow With MauriceFlex

Maurice Flex Workflow - 6 steps for protein fractionation based on ic-ief

How Protein Fractionation Works on MauriceFlex

  1. An applied electric field creates a pH gradient across the capillary inside the MauriceFlex cartridge
  2. Proteins separate by migrating to different pH values on the gradient, based on their individual isoelectric point (pI)
  3. Introduction of ammonium acetate changes the pH gradient, mobilizing these separated proteins and resulting in elution (fractionation)
Charge variant fraction collection process on MauriceFlex is faster than IEX
icIEF protein fractions collected on MauriceFlex are compatible with mass spec analysis
A. icIEF profiles of charge isoforms. B. Mobilization of charge isoform peaks for fractionation

Which Maurice is Right for You?

 

System

Maurice

Maurice C.

Maurice S.

Maurice Flex

icIEF Charge Application

Yes

Yes

No

 Yes

CE-SDS Size Application

Yes

No

Yes

 Yes
icIEF Fractionation No No No Yes

Absorbance Detection

Yes

Yes

Yes

 Yes

Fluorescence Detection

Yes

Yes

No

 Yes

Onboard Mixing for Sample Prep

Yes

Yes

No

 No

Specifications

Description

cIEF

CE-SDS PLUS

Turbo CE-SDS

cIEF Fractionation

Minimum Sample Volume

50 µL

50 µL

100 µL

 100 µL

Sample Delivery

Vacuum

Electrokinetic

Electrokinetic

 Vacuum

Typical Separation Time

6-10 min (molecule-dependent)

Reduced IgG: 25 minutes; Non-reduced IgG: 35 minutes

Reduced IgG: 5.5 minutes; Non-reduced IgG: 8 minutes

 40 – 50 min (molecule-dependent)
Detection Capability UV Absorbance at 280 nm

Fluorescence: Ex 280 nm, Em 320–450 nm		 UV Absorbance at 220 nm UV Absorbance at 220 nm Fluorescence: Ex 280 nm, Em 320-450nm

Typical Voltage

Pre-focusing: 1500 V
Focusing: 3000 V

Separation: 5750 V

Separation: 4,200 V

 Pre-focusing: 500 V and 1000 V
Focusing: 1500 V

Sample Injections per Cartridge

100 guaranteed, 200 maximum (max 25 batches)

100 guaranteed, 500 maximum (max 25 batches)

100 guaranteed (max 25 batches)

 Maximum 15 injections

Maximum Sample Injections per Batch

100

48

96

 1 (fractionation)
4 (cIEF)
 

pI/Size Range

2.85-10.45

10-270 kDa

10-270 kDa

 3-10

pI/Sizing CV

1%

≤2%

<2%

 1%

CV for Peaks >10% Composition

≤5% (Intra-batch), ≤6% (Inter-batch)

N/A

N/A

 ≤10% (Inter-batch)
Relative Migration Time CV N/A <1% for reduced IgG <5% N/A

pI/Sizing Resolution

0.05 pl units (for wide range 3-10 ampholyte)

≥ 1.5 for NGHC/HC IgG Standard)

≥ 1.0 for NGHC/HC IgG Standard)

 N/A

Dynamic Range

2 logs

2 logs

2 logs

 N/A

Linearity

>0.995

>0.995

>0.995

 N/A

Sensitivity (LOD)

0.7 µg/mL (Native fluorescence)
3.0 µg/mL (Absorbance) (Values based on a monoclonal antibody)

0.3 µg/mL
(Value based on internal standard)

0.6 μg/mL (value based on internal standard)

 N/A

Sample Tray Options

96-well plates or 48 vials

96-well plates or 48 vials

96-well plates or 48 vials

 96-well plates only

Power

100 V-240 V (AC), 50/60 Hz, 500 W

100 V-240 V (AC), 50/60 Hz, 500 W

100 V-240 V (AC), 50/60 Hz, 500 W

 100 V-240 V (AC), 50/60 Hz, 500 W
Voltage Range 0–6,500 V 0–6,500 V 0–6,500 V 0–6,500 V

Temperature Control Range

4-25 °C

4-25 °C

4-25 °C

 10-25 °C

Dimensions

44cm H x 42cm W x 61cm D

44cm H x 42cm W x 61cm D

44cm H x 42cm W x 61cm D

 44cm H x 42cm W x 61cm D

Weight

46 kg (100 lb)

46 kg (100 lb)

46 kg (100 lb)

 46 kg (100 lb)