Flex Your Freedom
Introducing MauriceFlex, your new protein fractionation solution that lets you collect fractions for charge variant analysis. MauriceFlex gives you:
- Freedom to do more with your fractions – analyze with intact mass, reduced and subunit mass, peptide mapping, and more
- Freedom from laborious IEX and HPLC workflows – easily collect high-purity fractions without separate extensive development of chromatography-based fractionation
- Freedom to do it all - icIEF, CE-SDS, and icIEF protein fractionation all in one instrument
- Freedom to do more with your time - identify, separate, and fractionate charge variants in less than a day
Table of Contents
- Are You Using IEX or HPLC for Protein Fractionation
- Featured Resources
- icIEF Protein Fractionation Workflow With MauriceFlex
- How Protein Fractionation Works on MauriceFlex
- Which Maurice is Right for You?
MauriceFlex combines protein charge isoform fractionation with imaged-capillary isoelectric focusing (icIEF) and CE-SDS analysis in one instrument, so that you can go beyond routine size and charge and conduct charge variant analysis of your molecule at any stage without needing to rely on different methods like ion-exchange chromatography (IEX) and HPLC.
Are You Using IEX or HPLC for Protein Fractionation?
Protein fractionation with MauriceFlex renders significant advantages on time and capital when compared with IEX, HPLC and other existing technologies. The key advantages are:
- Same-day fraction collection
- High purity fractions
- Single fractionation offering enough material for intact MS analysis
- Compatibility with urea and methylcellulose
- Ability to pool fractions for peptide mapping
- Same system offering CE-SDS and cIEF assays
- Eliminating the need for bridging studies
Featured Resources
Application Spotlight
Explore how NIST mAb samples were fractionated on MauriceFlex and further analyzed with LC-MS for intact mass and peptide mapping.
Scientific Poster
Learn about novel icIEF Fractionation Coupled with Different MS Systems for Rapid Charge Variant Analysis of Therapeutic Antibodies
icIEF Protein Fractionation Workflow With MauriceFlex
How Protein Fractionation Works on MauriceFlex
- An applied electric field creates a pH gradient across the capillary inside the MauriceFlex cartridge
- Proteins separate by migrating to different pH values on the gradient, based on their individual isoelectric point (pI)
- Introduction of ammonium acetate changes the pH gradient, mobilizing these separated proteins and resulting in elution (fractionation)
Which Maurice is Right for You?
System |
Maurice |
Maurice C. |
Maurice S. |
Maurice Flex |
---|---|---|---|---|
icIEF Charge Application |
Yes |
Yes |
No |
Yes |
CE-SDS Size Application |
Yes |
No |
Yes |
Yes |
icIEF Fractionation | No | No | No | Yes |
Absorbance Detection |
Yes |
Yes |
Yes |
Yes |
Fluorescence Detection |
Yes |
Yes |
No |
Yes |
Onboard Mixing for Sample Prep |
Yes |
Yes |
No |
No |
Specifications
Description |
cIEF |
CE-SDS PLUS |
Turbo CE-SDS |
cIEF Fractionation |
---|---|---|---|---|
Minimum Sample Volume |
50 µL |
50 µL |
100 µL |
100 µL |
Sample Delivery |
Vacuum |
Electrokinetic |
Electrokinetic |
Vacuum |
Typical Separation Time |
6-10 min (molecule-dependent) |
Reduced IgG: 25 minutes; Non-reduced IgG: 35 minutes |
Reduced IgG: 5.5 minutes; Non-reduced IgG: 8 minutes |
40 – 50 min (molecule-dependent) |
Detection Capability | UV Absorbance at 280 nm Fluorescence: Ex 280 nm, Em 320–450 nm |
UV Absorbance at 220 nm | UV Absorbance at 220 nm | Fluorescence: Ex 280 nm, Em 320-450nm |
Typical Voltage |
Pre-focusing: 1500 V |
Separation: 5750 V |
Separation: 4,200 V |
Pre-focusing: 500 V and 1000 V Focusing: 1500 V |
Sample Injections per Cartridge |
100 guaranteed, 200 maximum (max 25 batches) |
100 guaranteed, 500 maximum (max 25 batches) |
100 guaranteed (max 25 batches) |
Maximum 15 injections |
Maximum Sample Injections per Batch |
100 |
48 |
96 |
1 (fractionation) 4 (cIEF) |
pI/Size Range |
2.85-10.45 |
10-270 kDa |
10-270 kDa |
3-10 |
pI/Sizing CV |
1% |
≤2% |
<2% |
1% |
CV for Peaks >10% Composition |
≤5% (Intra-batch), ≤6% (Inter-batch) |
N/A |
N/A |
≤10% (Inter-batch) |
Relative Migration Time CV | N/A | <1% for reduced IgG | <5% | N/A |
pI/Sizing Resolution |
0.05 pl units (for wide range 3-10 ampholyte) |
≥ 1.5 for NGHC/HC IgG Standard) |
≥ 1.0 for NGHC/HC IgG Standard) |
N/A |
Dynamic Range |
2 logs |
2 logs |
2 logs |
N/A |
Linearity |
>0.995 |
>0.995 |
>0.995 |
N/A |
Sensitivity (LOD) |
0.7 µg/mL (Native fluorescence) |
0.3 µg/mL |
0.6 μg/mL (value based on internal standard) |
N/A |
Sample Tray Options |
96-well plates or 48 vials |
96-well plates or 48 vials |
96-well plates or 48 vials |
96-well plates only |
Power |
100 V-240 V (AC), 50/60 Hz, 500 W |
100 V-240 V (AC), 50/60 Hz, 500 W |
100 V-240 V (AC), 50/60 Hz, 500 W |
100 V-240 V (AC), 50/60 Hz, 500 W |
Voltage Range | 0–6,500 V | 0–6,500 V | 0–6,500 V | 0–6,500 V |
Temperature Control Range |
4-25 °C |
4-25 °C |
4-25 °C |
10-25 °C |
Dimensions |
44cm H x 42cm W x 61cm D |
44cm H x 42cm W x 61cm D |
44cm H x 42cm W x 61cm D |
44cm H x 42cm W x 61cm D |
Weight |
46 kg (100 lb) |
46 kg (100 lb) |
46 kg (100 lb) |
46 kg (100 lb) |