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MFI™ gives you more insight into the world of sub-visible particles—way more than just size and count. Because it's image-based, you also get quantifiable morphological parameters for your analysis. Easily differentiate and characterize different particle populations in no time. Plus, MFI has an optic focal length that spans the entire fluidic path, so your sample data is robust and reproducible day in and day out. Add a Bot1 anytime to automate the whole process and kick your throughput up a notch. If you're looking for broader, deeper analysis of your biotherapeutic products, MFI is the particle analysis tool for you.

ProteinSimple is now a brand in the Bio-Techne family of brands that includes R&D Systems, Novus Biologicals and Tocris. As a company, Bio-Techne has developed, manufactured and sold biotechnology products, clinical calibrators and controls, and consumables for protein analysis—and now analytical instrumentation and consumables through ProteinSimple. 


Micro-Flow Imaging MFI

MFI 5000

Detect sub-visible particles

MFI consumables by ProteinSimple a Bio-Techne brand

MFI Consumables

You can now order consumables for MFI online

MFI Virtual Demo

MFI Virtual Demo

See how MFI easily detects, quantitates, and provides morphological data on subvisible particles

How does MFI work?

Micro-Flow Imaging combines the direct imaging capabilities of digital microscopy with the precise control of microfluidics. What does that get you? High-resolution images with 85% sampling efficiency, more precise counts and sizing with full morphological detail for all sub-visible particles in your sample and the complete confidence that you can accurately identify every possible type, from protein aggregates to air bubbles.

Images of the sample are captured as it passes through the flow cell's sensing zone. Every particle in every image is then analyzed to create a database of particle count, size, transparency and morphology (or shape). And you'll have visual verification on the spot, as images are displayed in real-time. You can also display the results for many samples at once, which makes it easy to monitor stability and comparability. 

MFI Diagram of the Micro-Flow Imaging Process Including Digital Camera, Optics, Samples, and Flow Cell

MFI Systems Comparison Chart


MFI 5100

MFI 5200

Size Range

2 to 300 µm

1 to 70 µm

Percent of Sample Analyzed

>85% - entire size range

>85% - entire size range

Depth of Field (DOF)

400 µm

100 µm

Flow Cell Depth

400 µm - DOF matched

100 µm - DOF matched

Analysis Rate

200 µL/min

150 µL/min

Maximum Concentration (@2.5 µm)

175,000 particles/mL

900,000 particles/mL

MFI Applications



Enhance the safety and efficacy of your formulation and final product by detecting, quantifying, and classifying different sub-visible particles such as protein aggregates, silicone oil, and other contaminants.

Cell & Gene Therapy

Cell & Gene Therapy

Next-generation analytical instrumentation to help improve and accelerate your cell and gene therapy workflows.

Protein Aggregation and Particle Analysis

Protein Aggregation & Particle Analysis

Improve the safety of your therapeutic with accurate detection and characterization of translucent protein aggregates and other particulates.



Be assured of your biosimilar’s stability through the reproducible detection and quantitation of particulate matter and aggregates during development, formulation, and manufacturing.


Vaccine Development

Develop and manufacture safe vaccines by accurately visualizing and classifying different particle contaminants in your product, from development to delivery.