Properties and Applications of MitoBrilliant Dyes

MitoBrilliant probes have been developed to overcome some common limitations encountered with standard mitochondrial trackers. The range includes 2 types of dyes, MitoBrilliant Live dyes, suitable for live-cell imaging; and a ‘universal’ dye, suitable for both live-cell and fixed-cell imaging.

MitoBrilliant 646 and leading competitor dye, live-cell time lapse imaging

Excellent Brightness and Photostability

MitoBrilliant Live 646 (Cat. No. 7417) produced a more stable, longer-lasting fluorescent signal than a leading competitor dye in live-cell time lapse imaging.

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MitoBrilliant 646 signal was unchanged after CCCP treatment while accumulation of competitor dye was reversible

Δψm-Dependent and Δψm-Independent Mitochondrial Staining

  • MitoBrilliant Live dyes (Cat. No. 7417 and 7693) accumulate in the mitochondria of live cells in a Δψm dependent manner.
  • MitoBrilliant 646 (Cat. No. 7700) can be retained in mitochondria when Δψm is disrupted.

 

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Improved Mitochondrial Staining Fidelity Post-Fixation

Significantly improved data quality from staining with MitoBrilliant 646 vs competitor dye on HeLa cells fixed with PFA

 

MitoBrilliant 646 (Cat. No. 7700) staining is retained after fixation with PFA and provides significantly improved staining resolution than a leading competitor dye.

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MitoBrilliant Dyes Support a Wide Range of Applications

MitoBrilliant dyes enable highly accurate, sensitive, specific and persistent labeling of mitochondria in a wide variety of applications.

 

MitoBrilliant dyes enable highly accurate, sensitive, specific and persistent labeling of mitochondria in a wide variety of applications.

MitoBrilliant Technology - Key Features

 

Product Name

Abs/Em
(nm)

Δψm
Dependent

Live/Fixed
Cell Use?

 Stains
Pre-Fixed
Cells?		 Image Without
Wash Step?		 Demonstrated Applications

MitoBrilliant 646

Cat. No. 7700

655/668

No

Suitable for live and fixed cell work

Yes

Yes, but replacing media recommended

Fixed-cell imaging, Live-cell imaging Flow cytometry, IHC/ICC,

Super-resolution microscopy (STED), High-content screening

MitoBrilliant Live 646

Cat. No. 7417

648/662

Yes

Live-cell work only

No

 Yes, but replacing media recommended

Live-cell imaging, Flow cytometry, High-content screening

MitoBrilliant Live 549

Cat. No. 7693

 550/568 Yes Live-cell work only

No

 Yes, but replacing media recommended

Live-cell imaging, Flow cytometry, High-content screening

MitoBrilliant Product Guide

 

Our guide provides protocols and data for use in different research applications including flow cytometry, immunohistochemistry, live-cell and fixed-cell imaging, and super-resolution microscopy. Please download for more information.

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