Mouse IL-17 Quantikine HS ELISA Kit

Name: Mouse IL-17 Quantikine HS ELISA Kit
Brand: R&D Systems
SKU: MHS170

Catalog # MHS170 | R&D Systems, Inc. a Bio-Techne Brand

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Key Product Details

Assay Length:	4.0 hours
Sample Type & Volume Required Per Well:	Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity:	0.214 pg/mL
Assay Range:	0.781 - 50 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Mouse IL-17 Quantikine HS ELISA Kit Summary:	The Quantikine™ HS Mouse IL-17 Immunoassay is a 4.0 hour solid phase ELISA designed to measure mouse IL-17 levels in serum and plasma. It contains E. coli-expressed recombinant mouse IL-17 and antibodies raised against the recombinant protein. Results obtained using natural IL-17 showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that the Quantikine HS Immunoassay kit can be used to determine relative mass values for natural mouse IL-17.

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Technical Data
Citations / Reviews

Product Specifications for Mouse IL-17 Quantikine HS ELISA Kit

Measurement	Quantitative ELISA
Detection Method	Colorimetric - 450 or 490nm (TMB or AP Substrate)
Conjugate	HRP
Reactivity	Mouse
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	6.82	16.9	31.9	6.92	16.6	31.4
Standard Deviation	0.239	0.429	0.836	0.349	0.814	1.64
CV%	3.5	2.5	2.6	5.1	4.9	5.2

Recovery

The recovery of mouse IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	90	78-96
Heparin Plasma (n=4)	89	80-97
Serum (n=4)	89	75-98

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of mouse IL-17 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Examples for Mouse IL-17 Quantikine HS ELISA Kit

	Mouse IL-17 Quantikine HS ELISA Standard Curve

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-17/IL-17A

The IL-17 family is comprised of at least six proinflammatory cytokines that share a conserved cysteine-knot structure but diverge at the N-terminus. IL-17 family members are glycoproteins secreted as dimers that induce local cytokine production and recruit granulocytes to sites of inflammation. IL-17 is induced by IL-15 and IL-23, mainly in activated CD4+ T cells distinct from Th1 or Th2 cells. IL-17F is the most homologous to IL-17, but is induced only by IL-23 in activated monocytes. IL-17B binds the IL-17B receptor, but not the IL-17 receptor; it is most homologous with IL-17D, which is expressed by resting CD4+ T cells and CD19+ B cells. IL-17E is mainly produced by Th2 cells and recruits eosinophils to lung tissue. IL-17C has a very restricted expression pattern but has been detected in adult prostate and fetal kidney libraries.

Item	Value
Long Name	Interleukin 17
Entrez Gene IDs	3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)
Alternate Names	CTLA-8, CTLA8, CTLA8cytotoxic T-lymphocyte-associated serine esterase 8, Cytotoxic T-lymphocyte-associated antigen 8, IL-17, IL-17A, IL-17Acytotoxic T-lymphocyte-associated protein 8, IL-17CTLA-8, IL17, IL17A, IL17interleukin-17A, interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8), interleukin 17A

Citations for Mouse IL-17 Quantikine HS ELISA Kit

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Mouse IL-17 Quantikine HS ELISA Kit

Catalog # MHS170 | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Product Specifications for Mouse IL-17 Quantikine HS ELISA Kit

Precision

Recovery

Linearity

Scientific Data Examples for Mouse IL-17 Quantikine HS ELISA Kit

Preparation and Storage

Background: IL-17/IL-17A

Citations for Mouse IL-17 Quantikine HS ELISA Kit

Product Documents for Mouse IL-17 Quantikine HS ELISA Kit

Product Datasheets

COA

Certificate of Analysis

SDS

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