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Neuron-specific beta‑III Tubulin Antibody Best Seller

Catalog # MAB1195 | R&D Systems, Inc. a Bio-Techne Brand
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MAB1195
MAB1195-SP

Key Product Details

Species Reactivity

Multi-Species

Applications

Immunocytochemistry, Immunohistochemistry, Intracellular Staining by Flow Cytometry, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # TuJ-1

Product Summary for Neuron-specific beta‑III Tubulin Antibody

Immunogen

Rat brain-derived microtubules

Specificity

Detects mammalian and chicken neuron-specific beta-III tubulin but not other beta-tubulin isotypes in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Neuron-specific beta‑III Tubulin Antibody

Detection of Human and Mouse  beta-III Tubulin antibody by Western Blot.

Detection of Human and Mouse beta‑III Tubulin by Western Blot.

Western blot shows lysates of human brain (cerebellum) tissue, human brain (hypothalamus) tissue, mouse brain (cerebellum) tissue, and mouse brain (stem) tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Neuron-specific beta-III Tubulin Monoclonal Antibody (Catalog # MAB1195) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for beta-III Tubulin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
beta‑III Tubulin antibody in Rat Cortical Neurons and GFAP antibody in Rat Astrocytes by Immunocytochemistry (ICC).

beta‑III Tubulin in Rat Cortical Neurons and GFAP in Rat Astrocytes.

beta-III Tubulin was detected in rat cortical neurons using 5 µg/mL neuron-specific Mouse Anti-Neuron-specific beta-III Tubulin Monoclonal Antibody (Catalog # MAB1195). GFAP was detected in rat astrocytes using 10 µg/mL Human GFAP Antigen Affinity-purified Polyclonal Antibody (AF2594). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for beta-III Tubulin using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and for GFAP using the NorthernLights 493-conjugated Anti-Sheep IgG Secondary Antibody (green; NL012). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
beta‑III Tubulin antibody in Differentiated Human Neural Progenitor Cells by Immunocytochemistry (ICC).

beta‑III Tubulin in Differentiated Human Neural Progenitor Cells.

beta‑III Tubulin was detected in immersion fixed differentiated human neural progenitor cells using Mouse Anti-Neuron-specific beta‑III Tubulin Monoclonal Antibody (clone TuJ-1) (Catalog # MAB1195) for 3 hours at room temperature. Cells were stained (green) and counterstained (red). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Neuron-specific beta‑III Tubulin Antibody

Application
Recommended Usage

Immunocytochemistry

0.5-25 µg/mL
Sample: Immersion fixed rat cortical stem cells, rat cortical neurons, SH‑SY5Y human neuroblastoma cells (Positive) and HEL 92.1.7 human erythroleukemic cell line (Negative)

Immunohistochemistry

1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: HepG2 human hepatocellular carcinoma cells

Simple Western

10 µg/mL
Sample: Rat cortical neurons

Western Blot

0.2 µg/mL
Sample: Human brain (cerebellum) tissue, human brain (hypothalamus) tissue, mouse brain (cerebellum) tissue, and mouse brain (stem) tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 20 reviews rated 4.7 using MAB1195 in the following applications:

Published Applications

Read 211 publications using MAB1195 in the following applications:

Formulation, Preparation and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-III Tubulin

beta-III Tubulin, also known as tubulin beta-4, is regarded as a neuron-specific marker. The expression of beta-III Tubulin has been suggested to be one of the earliest markers to signal commitment in primitive neuroepithelium.

Alternate Names

beta-Tubulin III, Beta3-tubulin, betaIII Tubulin, Class III beta-tubulin, TUBB3, Tubulin Beta 3, Tubulin beta-4, Tubulin beta-III

Entrez Gene IDs

10381 (Human)

Gene Symbol

TUBB3

Product Documents for Neuron-specific beta‑III Tubulin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Neuron-specific beta‑III Tubulin Antibody

For research use only

Citations for Neuron-specific beta‑III Tubulin Antibody (211)

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Customer Reviews for Neuron-specific beta‑III Tubulin Antibody (20)

4.7 out of 5
20 customer ratings
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Showing  1 - 5 of 20 reviews Showing All
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  • Neuron-specific beta-III Tubulin Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Fibroblast-like cells
    Species: Mouse
    Verified Customer | Posted 07/13/2021
    Neuron-specific  beta‑III Tubulin Antibody MAB1195
  • Neuron-specific beta-III Tubulin Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: motor neuron
    Species: Mouse
    Verified Customer | Posted 06/28/2021
    Neuron-specific  beta‑III Tubulin Antibody MAB1195
  • Neuron-specific beta-III Tubulin Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Cortical neurons
    Species: Mouse
    Verified Customer | Posted 05/04/2021
    Neuron-specific  beta‑III Tubulin Antibody MAB1195
  • Neuron-specific beta-III Tubulin Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Embryo
    Species: Mouse
    Verified Customer | Posted 11/22/2019
    Analysis of sensory fibre growth in vivo was carried out using iDISCO preparations (Renier et al., 2014, using RnD MAB1195,1:300, and anti-mouse 546 Alexa Fluor secondary antibody 1:300. Fantastic antibody across many applications.
    Neuron-specific  beta‑III Tubulin Antibody MAB1195
  • Neuron-specific beta -III Tubulin Antibody
    Name: Anonymous
    Application: Immunofluorescence Acetone fixed
    Sample Tested: Adult mouse brain tissue
    Species: Mouse
    Verified Customer | Posted 01/17/2019
    Neuron-specific  beta‑III Tubulin Antibody MAB1195
  • Neuron-specific beta -III Tubulin Antibody
    Name: Fatemeh Naini
    Application: Simple Western
    Sample Tested: Cortical neurons
    Species: Human
    Verified Customer | Posted 11/27/2018
    Neuron-specific  beta‑III Tubulin Antibody MAB1195
  • Neuron-specific beta -III Tubulin Antibody
    Name: Danlee Enzler
    Application: Immunohistochemistry
    Sample Tested: Cortical stem cells
    Species: Rat
    Verified Customer | Posted 04/24/2018
  • Neuron-specific beta -III Tubulin Antibody
    Name: Leslie Priddy
    Application: Immunohistochemistry
    Sample Tested: Serum
    Species: Mouse
    Verified Customer | Posted 04/13/2018
  • Neuron-specific beta -III Tubulin Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: IPS2 induced pluripotent stem cells
    Species: Mouse
    Verified Customer | Posted 01/25/2018
    Neuron-specific  beta‑III Tubulin Antibody MAB1195
  • Neuron-specific beta -III Tubulin Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: IPS2 induced pluripotent stem cells
    Species: Mouse
    Verified Customer | Posted 01/25/2018
    Neuron-specific  beta‑III Tubulin Antibody MAB1195
  • Neuron-specific beta-III Tubulin Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Adult brain
    Species: Feline
    Verified Customer | Posted 11/02/2017
    Neuron-specific  beta‑III Tubulin Antibody MAB1195
  • Neuron-specific beta -III Tubulin Antibody
    Name: Michelle Chen
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Human fibroblast and human neural stem cells
    Species: Human
    Verified Customer | Posted 05/10/2017
  • Neuron-specific beta -III Tubulin Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cortical neurons
    Species: Mouse
    Verified Customer | Posted 04/23/2017
  • Neuron-specific beta -III Tubulin Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Neural progenitor cells
    Species: Human
    Verified Customer | Posted 04/13/2017
    Neuron-specific  beta‑III Tubulin Antibody MAB1195
  • Name: cynthia morimoto
    Application: Western Blot
    Sample Tested: See PMID 23158765
    Species: Mouse
    Verified Customer | Posted 02/04/2015
  • Name: cynthia morimoto
    Application: Immunofluorescence
    Sample Tested: See PMID 19409493
    Species: Mouse
    Verified Customer | Posted 02/04/2015
  • Name: cynthia morimoto
    Application: Immunofluorescence
    Sample Tested: See PMID 22985741
    Species: Human
    Verified Customer | Posted 02/04/2015
  • Name: cynthia morimoto
    Application: Immunocytochemistry
    Sample Tested: See PMID 20722977
    Species: Mouse
    Verified Customer | Posted 02/04/2015
  • Name: cynthia morimoto
    Application: Immunohistochemistry-Frozen
    Sample Tested: See PMID 22120963
    Species: Other
    Verified Customer | Posted 02/04/2015
  • Name: cynthia morimoto
    Application: Immunofluorescence
    Sample Tested: See PMID 23658023
    Species: Human
    Verified Customer | Posted 02/04/2015

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