PA Janelia Fluor^® 549, SE

Name: PA Janelia Fluor ® 549, SE
Brand: Tocris Bioscience
SKU: 6149

Catalog # 6149 | Tocris Bioscience a Bio-Techne Brand

Yellow photoactivatable dye (Reactive group: NHS ester). Applications: super resolution microscopy (PALM), single molecule tracking and live cell imaging

Key Product Details

Description:	Yellow photoactivatable dye (Reactive group: NHS ester). Applications: super resolution microscopy (PALM), single molecule tracking and live cell imaging
Chemical Name:	2,5-Dioxo-1-pyrrolidinyl 3',6'-di-1-azetidinyl-2-diazo-2,3-dihydro-3-oxospiro[1H-indene-1,9'-[9H]xanthene]-6-carboxylate
Purity:	≥90% (HPLC)
Molecular Weight:	575.58

View Full Technical Details for PA Janelia Fluor^® 549, SE

Technical Data
Citations (1) / Reviews

Biological Activity for PA Janelia Fluor^® 549, SE

PA Janelia Fluor^® 549, SE is a photoactivatable fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. Non-fluorescent until activated at 365 nm. Photochemical quantum yield of uncaging = 2.2%, with improved yield upon protein conjugation. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag^® and SNAP-tag^®. Suitable for single molecule tracking and super resolution microscopy in live cells, specifically live cell sptPALM and fixed cell PALM. Can be multiplexed with PA Janelia Fluor^® 646, SE (Cat. No. 6150) to perform two-color sptPALM in live cells with activation at 405 nm. Cell permeable. Excitation maximum = 551 - 553 nm; emission maximum = 570 - 573 nm.

Please see the protocol for further information on protein/antibody labeling and conjugation.

HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

Licensing Information

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data for PA Janelia Fluor^® 549, SE

M. Wt	575.58
Formula	C₃₂H₂₅N₅O₆
Storage	Store at -20°C
Purity	≥90% (HPLC)
CAS Number	1811539-42-0
PubChem ID	126569714
InChi Key	ZJZWRNGNJDEPEQ-UHFFFAOYSA-N
Smiles	O=C(ON1C(CCC1=O)=O)C2=CC=C(C(C3=[N+]=[N-])=O)C(C43C5=CC=C(N6CCC6)C=C5OC7=CC(N8CCC8)=CC=C47)=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for PA Janelia Fluor^® 549, SE

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	5.76	10

Preparing Stock Solutions for PA Janelia Fluor^® 549, SE

The following data is based on the product molecular weight 575.58.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	17.37 mL	86.87 mL	173.74 mL
0.5 mM	3.47 mL	17.37 mL	34.75 mL
1 mM	1.74 mL	8.69 mL	17.37 mL
5 mM	0.35 mL	1.74 mL	3.47 mL

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References for PA Janelia Fluor^® 549, SE

References are publications that support the biological activity of the product.

Grimm Bright photoactivatable fluorophores for single-molecule imaging. Nat.Methods 2016 PMID: 27776112
Li Real-time imaging of Huntingtin aggregates diverting target search and gene transcription. eLife 2016 PMID: 27484239
Hong Phosphorylation of the RNA polymerase II C-terminal domain by TFIIH kinase is not essential for transcription of Saccharomyces cerevisiae genome. Proc.Natl.Acad.Sci.USA. 2009 PMID: 19666497

Citations for PA Janelia Fluor^® 549, SE (1)

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Citations are publications that use Bio-Techne products. Selected citations for PA Janelia Fluor^® 549, SE include:

Genshaft et al. (2021) Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes Nat Commun. 12:4995
PMID: 34404785

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Product Documents for PA Janelia Fluor^® 549, SE

COA

Certificate of Analysis

To download a Certificate of Analysis, please select a batch below.

PA Janelia Fluor^® 549, SE

Catalog # 6149 | Tocris Bioscience a Bio-Techne Brand

Key Product Details

Biological Activity for PA Janelia Fluor^® 549, SE

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Technical Data for PA Janelia Fluor^® 549, SE

Solubility for PA Janelia Fluor^® 549, SE

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References for PA Janelia Fluor^® 549, SE

Citations for PA Janelia Fluor^® 549, SE (1)

Product Documents for PA Janelia Fluor^® 549, SE

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Certificate of Analysis

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PA Janelia Fluor® 549, SE

Catalog # 6149 | Tocris Bioscience a Bio-Techne Brand

Key Product Details

Biological Activity for PA Janelia Fluor® 549, SE

Licensing Information

Plan Your Experiments

Technical Data for PA Janelia Fluor® 549, SE

Solubility for PA Janelia Fluor® 549, SE

Preparing Stock Solutions for PA Janelia Fluor® 549, SE

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

References for PA Janelia Fluor® 549, SE

Citations for PA Janelia Fluor® 549, SE (1)

Product Documents for PA Janelia Fluor® 549, SE

COA

Certificate of Analysis

SDS

Bio-Techne Brands

PA Janelia Fluor^® 549, SE

Biological Activity for PA Janelia Fluor^® 549, SE

Technical Data for PA Janelia Fluor^® 549, SE

Solubility for PA Janelia Fluor^® 549, SE

Preparing Stock Solutions for PA Janelia Fluor^® 549, SE

References for PA Janelia Fluor^® 549, SE

Citations for PA Janelia Fluor^® 549, SE (1)

Product Documents for PA Janelia Fluor^® 549, SE