PA Janelia Fluor® 646, SE
Catalog # 6150 | Tocris Bioscience a Bio-Techne Brand
Red photoactivatable dye (Reactive group: NHS ester). Applications: super resolution microscopy (PALM), single molecule tracking and live cell imaging
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Key Product Details
Description: | Red photoactivatable dye (Reactive group: NHS ester). Applications: super resolution microscopy (PALM), single molecule tracking and live cell imaging |
Chemical Name: | 2,5-Dioxo-1-pyrrolidinyl 3,7-di-1-azetidinyl-2'-diazo-5,5-dimethyl-2',3'-dihydro-5H-3'-oxospiro[dibenzo[b,e]siline-10,1'-indene]-6'-carboxylate |
Purity: | ≥90% (HPLC) |
Molecular Weight: | 617.74 |
Citations for PA Janelia Fluor® 646, SE (3)
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Citations are publications that use Bio-Techne products. Selected citations for PA Janelia Fluor® 646, SE include:
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Jeroen et al.
(2022)
Microscopy-based single-cell proteomic profiling reveals heterogeneity in DNA damage response dynamics.
Cell Rep Methods.
2:100237
PMID: 35784653 -
Genshaft et al.
(2021)
Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
Nat Commun.
12:4995
PMID: 34404785 -
Sheila S et al.
(2019)
Visualizing Transcription Factor Binding on Mitotic Chromosomes Using Single-Molecule Live-Cell Imaging.
Methods Mol Biol.
2038:239-250
PMID: 31407289
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Customer Reviews for PA Janelia Fluor® 646, SE (1)
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Species: OtherAssay Type: In VitroThis product was used to label recombinant proteins and image them with single molecule sensitivity. The degree of labeling achieved was 1.4 (average). The photoactivation efficiency of the dye is excellent and can be used to track a few molecules at a time, for long periods. The dye is highly photostable and can be used to track proteins for ~10-15 seconds under ideal tracking conditions. I attach here an image before and after photoactivation of the dye. Upon photoactivation, single dye molecules conjugated to the protein are visible.
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