PEI STAR™ transfection reagent

Name: PEI STAR™ transfection reagent
Brand: Tocris Bioscience
SKU: 7854

Catalog # 7854 | Tocris Bioscience a Bio-Techne Brand

COA / SDS

Polyethylenimine (PEI) transfection reagent, chemically-defined

Key Product Details

Description:	Polyethylenimine (PEI) transfection reagent, chemically-defined
Chemical Name:	Poly[imino(1,2-ethanediyl)] hydrochloride

View Full Technical Details for PEI STAR™ transfection reagent

Technical Data
Citations / Reviews

Biological Activity for PEI STAR™ transfection reagent

PEI STAR™ is a chemically-defined, high-performance polyethylenimine (PEI) transfection reagent for cost-effective, affordable and scalable transient gene expression. PEI is a synthetic polymer with an exceptionally high positive charge density in pH-neutral solutions. Positively charged PEI binds strongly to negatively charged DNA and imparts a net cationic charge, allowing the DNA to enter cells. PEI is a non-viral vector commonly used to transfect HEK293 and CHO cells. Applications include production of recombinant proteins, antibodies and viruses.

PEI STAR is a trademark of Bio-Techne Corp.

Protocols

Please see the links below for protocols relating to the use of PEI STAR^™

Protocol Name	Cell Type
PEI STAR^™ Transfection Reagent preparation
Gene Expression in Adherent HEK293 cells	Adherent
rAAV Production in Adherent HEK293 Cells	Adherent
Lentivirus Production in Adherent HEK293T Cells	Adherent
Gene Expression in HEK293 Cell Suspensions	Suspension
rAAV Production in Suspension HEK293 Cells	Suspension
Gene Expression in CHO Suspensions	Suspension

Scientific Data Examples for PEI STAR™ transfection reagent

	Application of PEI STAR™ transfection reagent. PEI STAR™ performance data comparison (HEK293): HEK 293 - 20 mL cultures containing HEK293 suspensions were transfected with a CMV-SEAP plasmid at optimized PEI/DNA ratios using either PEI STAR™ (3:1) or leading competitor PEI. SEAP expression levels were quantified 5 days post-transfection using phosphatase reporter dye and UV/Vis absorbance.
	Application of PEI STAR™ transfection reagent. PEI STAR™ performance data comparison (CHO): CHO - 20 mL cultures containing CHO suspensions were transfected with a CMV-SEAP plasmid at optimized PEI/DNA ratios using either PEI STAR™ (5:1) or leading competitor PEI. SEAP expression levels were quantified 5 days post-transfection using phosphatase reporter dye and UV/Vis absorbance.

Technical Data for PEI STAR™ transfection reagent

Storage	Store at RT
CAS Number	49553-93-7

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for PEI STAR™ transfection reagent

References are publications that support the biological activity of the product.

Trivedi Comparison of highly pure rAAV9 vector stocks produced in suspension by PEI transfection or HSV infection reveals striking quantitative and qualitative differences. Mol.Ther.Methods Clin.Dev. 2021 PMID: 35071688
Han Aberrant role of pyruvate kinase M2 in the regulation of gamma-secretase and memory deficits in Alzheimer's disease. Cell Rep. 2021 PMID: 34879266
Longo Transient mammalian cell transfection with polyethylenimine (PEI). Methods Enzymol. 2013 PMID: 24011049
Huang AAV2 production with optimized N/P ratio and PEI-mediated transfection results in low toxicity and high titer for in vitro and in vivo applications. J.Virol.Methods 2013 PMID: 23791963
Boussif A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine. Proc.Natl.Acad.Sci.U.S.A 1995 PMID: 7638184

Citations for PEI STAR™ transfection reagent

Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.