Post-translational Modification Characterization

Post-translation modifications of proteins are immunodetected using Simple Western assays as they’re separated by charge or by size using isoform specific antibodies. And, as an added bonus, Simple Western assays also let you use pan or total immunodetecting antibodies to monitor protein isoforms and post-translation modifications during charge-based separations. Try that with any other method!

Because many post-translational modifications result in a change in isoelectric point, they’re easily separated and quantitated on our iCE systems. You’ll be able to resolve a whole host of charge variants like deamidation, C-terminal lysines, sialylation, oxidation, glycoslyation glycation and any other change that results in a pI shift. Free solution IEF takes place in a capillary column (cIEF) to let you detect focused protein zones in 10 minutes flat!