Could you tell us whether this MMP-9 antibody can detect TIMP and MMP-9 complex?

Name: Proteome Profiler Human Protease Array Kit
Brand: R&D Systems
SKU: ARY021B

We haven't performed a large amount of specificity testing per each spot in the arrays because these are qualitative screening tools. However, we would recommend treating the MMP-9 spot as a "Total MMP-9" detecting both free MMP-9 and MMP-9 in complex. If the MMP-9 spot turns out to be of high interest for the customer's study, they will want to examine this target in more detail with quantitative tools such as ELISA or Luminex.

Proteome Profiler Human Protease Array Kit

Catalog # ARY021B | R&D Systems, Inc. a Bio-Techne Brand

Citations (24)

Product Datasheet / COA / SDS

Contains 4 membranes - each spotted in duplicate with 35 different protease antibodies

Key Product Details

Setting the standard in quality research reagents

Technical Data
Citations (24) / Reviews / FAQs

Summary for Proteome Profiler Human Protease Array Kit

Kit Summary

To simultaneously detect the relative changes of 35 proteases in a single sample. No specialized equipment is necessary

Troubleshooting Guide

General Assay Principle

Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernates, cell lysates, serum, plasma, urine, saliva, or tissue lysate samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any protein/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of protein bound. Chemiluminescence is detected in the same manner as a Western blot.

Kit Contents

4 Array Membranes
4-Well Multi-dish
Array Buffers
Wash Buffer
Detection Antibody Cocktail
Streptavidin-HRP
Chemiluminescent Detection Reagents
Transparency Overlay Template
Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the Product Datasheet.

Stability and Storage

Store the unopened product at 2^° C to 8^° C. Do not use past expiration date.

Simultaneously detect the relative levels of these proteases in a single sample.
ADAM8	Cathepsin X/Z/P	MMP-7
ADAM9	DPPIV/CD26	MMP-8
ADAMTS1	Kallikrein 3/PSA	MMP-9
ADAMTS13	Kallikrein 5	MMP-10
Cathepsin A	Kallikrein 6	MMP-12
Cathepsin B	Kallikrein 7	MMP-13
Cathepsin C/DPPI	Kallikrein 10	Neprilysin/CD10
Cathepsin D	Kallikrein 11	Presenilin-1
Cathepsin E	Kallikrein 13	Proprotein Convertase 9
Cathepsin L	MMP-1	Proteinase 3
Cathepsin S	MMP-2	uPA/Urokinase
Cathepsin V	MMP-3

Assays for Analytes represented in the Human Protease Array Kit

Analyte	Quantikine^® ELISA Kits	DuoSet^® ELISA Development Systems
ADAM8		DY1031
ADAM9		DY939
ADAMTS1		DY2197
ADAMTS13	DADT130
Cathepsin A
Cathepsin B	DCATB0	DY953, DY2176
Cathepsin C/DPPI
Cathepsin D
Cathepsin E
Cathepsin L		DY952
Cathepsin S		DY1183, DY2227
Cathepsin V		DY1080
Cathepsin X/Z/P
DPPIV/CD26		DY1180
Kallikrein 3/PSA	DKK300	DY1344
Kallikrein 5	DKK500
Kallikrein 6
Kallikrein 7
Kallikrein 10
Kallikrein 11
Kallikrein 13
MMP-1	DMP100
MMP-2	MMP200	DY902
MMP-3	DMP300	DY513
MMP-7	DMP700	DY907
MMP-8	DMP800	DY908
MMP-9	DMP900	DY911
MMP-10	DM1000	DY910
MMP-12
MMP-13	DM1300	DY511, DY913
Neprilysin		DY1182
Presenilin-1		DY149
Proprotein Convertase 9	DPC900	DY3888
Proteinase 3		DY6134-05
uPA/Urokinase	DUPA00	DY1310

Product Specifications for Proteome Profiler Human Protease Array Kit

Species

Human

Preparation & Storage

Shipping Conditions	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Citations for Proteome Profiler Human Protease Array Kit (24)

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Citations are publications that use Bio-Techne products. Selected citations for Proteome Profiler Human Protease Array Kit include:

Species: Human
Sample Types: Tissue Homogenates

Kim et al. (2023-05-19), Multiscale stiffness of human emphysematous precision cut lung slices Science advances
PMID: 37205750
Species: Human
Sample Types: Cell Culture Supernates

S Li et al. (2023-04-13), Galectin-3 promotes secretion of proteases that decrease epithelium integrity in human colon cancer cells Cell Death Disease, 2023-04-13;14(4):268.
PMID: 37055381
Species: Human
Sample Types: Cell Culture Supernates

E Mart Nez-T et al. (2023-03-23), Overexpressed Malat1 Drives Metastasis through Inflammatory Reprogramming of Lung Adenocarcinoma Microenvironment bioRxiv : the preprint server for biology, 2023-00(0).
PMID: 36993368
Species: Human
Sample Types: Cell Lysates

WY Aw et al. (2023-02-15), Microphysiological model of PIK3CA-driven vascular malformations reveals a role of dysregulated Rac1 and mTORC1/2 in lesion formation Science Advances, 2023-09(7):eade8939.
PMID: 36791204
Species: Human
Sample Types: Cell Culture Supernates

C Giannasi et al. (2022-06-10), Human Osteochondral Explants as an Ex Vivo Model of Osteoarthritis for the Assessment of a Novel Class of Orthobiologics Pharmaceutics, 2022-014(6).
PMID: 35745803
Species: Human
Sample Types: Cell Culture Supernates

Y Pan et al. (2022-02-11), Decidual macrophage derived MMP3 contributes to extracellular matrix breakdown in spiral artery remodeling in early human pregnancy Journal of reproductive immunology, 2022-0150(0):103494.
PMID: 35176662
Species: Human
Sample Types: Cell Culture Supernates

SW Shin et al. (2021-12-28), Anti-Periodontitis Effect of Ethanol Extracts of Alpinia Katsumadai Seeds Nutrients, 2021-114(1).
PMID: 35011011
Species: Human
Sample Types: Cell Culture Supernates

JB Candido et al. (2021-08-05), Kallikrein-Related Peptidase 6 Is Associated with the Tumour Microenvironment of Pancreatic Ductal Adenocarcinoma Cancers, 2021-013(16).
PMID: 34439122
Species: Human
Sample Types: Cell Lysates

YC Chuang et al. (2021-06-03), Pinosylvin inhibits migration and invasion of nasopharyngeal carcinoma cancer cells via regulation of epithelial?mesenchymal transition and inhibition of MMP?2 Oncology reports, 2021-046(1).
PMID: 34080661
Species: Human
Sample Types: Protein

F Valenzuela et al. (2020-11-26), Gingival Crevicular Fluid Zinc- and Aspartyl-Binding Protease Profile of Individuals with Moderate/Severe Atopic Dermatitis Biomolecules, 2020-110(12).
PMID: 33255937
Species: Human
Sample Types: Conditioned Media

X Zhang et al. (2019-08-27), Cancer-associated fibroblast stimulates cancer cell invasion in an interleukin-1 receptor (IL-1R)-dependent manner Oncol Lett, 2019-018(5):4645-4650.
PMID: 31611973
Species: Human
Sample Types: Cell Culture Supernates

LAN Crowe et al. (2019-02-06), S100A8 & S100A9: Alarmin mediated inflammation in tendinopathy Sci Rep, 2019-09(1):1463.
PMID: 30728384
Species: Human
Sample Types: Cell Culture Supernates

J Lee et al. (2019-01-01), Anti-invasive and Anti-tumor Effects of Dryopteris crassirhizoma Extract by Disturbing Actin Polymerization Integr Cancer Ther, 2019-018(0):1534735419851.
PMID: 31109222
Species: Human
Sample Types: Cell Culture Supernates

K Saito et al. (2018-07-30), Stromal mesenchymal stem cells facilitate pancreatic cancer progression by regulating specific secretory molecules through mutual cellular interaction J Cancer, 2018-09(16):2916-2929.
PMID: 30123360
Species: Human
Sample Types: Cell Lysates

SA Greenall et al. (2018-06-22), Differential Sensitivity of Human Hepatocellular Carcinoma Xenografts to an IGF-II Neutralizing Antibody May Involve Activated STAT3 Transl Oncol, 2018-011(4):971-978.
PMID: 29933129
Species: Human
Sample Types: Cell Lysates

DT Dao et al. (2018-06-07), Intranasal delivery of VEGF enhances compensatory lung growth in mice PLoS ONE, 2018-013(6):e0198700.
PMID: 29879188
Species: Human
Sample Types: Cell Culture Supernates

S Horng et al. (2017-07-24), Astrocytic tight junctions control inflammatory CNS lesion pathogenesis J. Clin. Invest., 2017-00(0).
PMID: 28737509
Species: Human
Sample Types: Cell Lysates

NPY Chung et al. (2017-05-09), HIV Reprograms Human Airway Basal Stem/Progenitor Cells to Acquire a Tissue-Destructive Phenotype Cell Rep, 2017-019(6):1091-1100.
PMID: 28494859
Species: Human
Sample Types: Cell Lysates

MJ Hsieh et al. (2017-03-22), Inhibition of cathepsin S confers sensitivity to methyl protodioscin in oral cancer cells via activation of p38 MAPK/JNK signaling pathways Sci Rep, 2017-07(0):45039.
PMID: 28327651
Species: Human
Sample Types: Cell Culture Supernates

(2016-12-23), Inhibition of radiation-induced glioblastoma invasion by genetic and pharmacological targeting of MDA-9/Syntenin Proc. Natl. Acad. Sci. U.S.A, 2016-10(0).
PMID: 28011764
Species: Human
Sample Types: Cell Lysates

Mohammed F et al. (2015-11-09), Blockade of voltage-gated sodium channels inhibits invasion of endocrine-resistant breast cancer cells. Int J Oncol, 2015-148(1):73-83.
PMID: 26718772
Species: Human
Sample Types: Cell Culture Supernates

Andrade B et al. (2015-08-12), Heme Oxygenase-1 Regulation of Matrix Metalloproteinase-1 Expression Underlies Distinct Disease Profiles in Tuberculosis. J Immunol, 2015-0195(6):2763-73.
PMID: 26268658
Species: Human
Sample Types: Cell Culture Supernates

Kumar D et al. (2014-10-12), Decidual GM-CSF is a critical common intermediate necessary for thrombin and TNF induced in-vitro fetal membrane weakening. Placenta, 2014-135(12):1049-56.
PMID: 25454284
Species: Human
Sample Types: Cell Culture Supernates

Rohrborn D et al. (2014-09-12), Shedding of dipeptidyl peptidase 4 is mediated by metalloproteases and up-regulated by hypoxia in human adipocytes and smooth muscle cells. FEBS Lett, 2014-0588(21):3870-7.
PMID: 25217834

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Showing 1 - 10 of 24 citations Showing All

FAQs for Proteome Profiler Human Protease Array Kit

Could you tell us whether this MMP-9 antibody can detect TIMP and MMP-9 complex?

We haven't performed a large amount of specificity testing per each spot in the arrays because these are qualitative screening tools. However, we would recommend treating the MMP-9 spot as a "Total MMP-9" detecting both free MMP-9 and MMP-9 in complex. If the MMP-9 spot turns out to be of high interest for the customer's study, they will want to examine this target in more detail with quantitative tools such as ELISA or Luminex.

Proteome Profiler Human Protease Array Kit

Catalog # ARY021B | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Summary for Proteome Profiler Human Protease Array Kit

General Assay Principle

Stability and Storage

Assays for Analytes represented in the Human Protease Array Kit

Product Specifications for Proteome Profiler Human Protease Array Kit

Preparation & Storage

Citations for Proteome Profiler Human Protease Array Kit (24)

FAQs for Proteome Profiler Human Protease Array Kit

Product Documents for Proteome Profiler Human Protease Array Kit

Product Datasheets

COA

Certificate of Analysis

SDS

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