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Trusted Data. Reliable Results.

Get results you can trust every time with Quantikine ELISA kits. We develop our ELISAs from raw materials that we manufacture in-house, giving us unparalleled control over critical elements that affect your results and ensure consistent performance over time.

Tight quality control and rigorous validation guarantees Quantikine ELISAs will perform with the ultimate precision, specificity, accuracy, and sensitivity.

  • Optimized and ready to use kits
  • Master calibrated for superior quality and reproducibility
  • Choose from over 450 ELISAs
  • The most referenced single analyte assay in literature

Extensively Validated for Reliable Performance

Stringent Quality Testing

Stringent Quality Testing

Superior accuracy and reproducibility has helped make Quantikine ELISA Kits the most trusted name in ELISAs, including intra- and inter-assay precision, assay linearity, and spike recovery, help ensure data accuracy for all validated sample types.  

TNF Control Values Graph

Legendary Consistency

Carefully selected antibody pairs and optimally formulated diluents help ensure accurate and reproducible results from one experiment to the next. Consistent control values established during assay development guarantees that your sample values will not shift or change over time.  

ELISA Citation

Most Cited ELISAs

With over 25,000 product citations, Quantikine ELISA Kits are the most referenced quantitative ELISA in the industry. Our database includes the sample type and a direct link to the research article, making it easy to review relevant references. You can trust our ELISAs to deliver results that will be reproducible tomorrow, next week, and next year.   

Broad ELISA Assay Offering

Custom ELISA services and custom assay services

Quantikine ELISAs are available in a range of formats including colorimetric, and chemiluminescence-based kits for measuring intracellular and extracellular analytes. Choose from over 450 optimized and ready to use kits to measure a wide range of molecules including cytokines, chemokines, growth factors, proteases and more.

Colorimetric Sandwich ELISA Kits

Accuracy You Can Count On. 
Complete ready-to-use colorimetric sandwich Quantikine ELISA kits are the most published ELISAs on the market and represent the gold standard in single analyte detection. Carefully selected antibody pairs, precision automated microplate coating, and optimally formulated diluents ensure accurate results.

Choose from over 450 assays in 9 species. Target analytes include: TNF alpha, IFN gamma, IL-6, IL-1 beta, IL-2, G-CSF and hundreds more. Kits are available for measuring a wide range of molecules including cytokines, chemokines, growth factors, proteases, and more.

Flexible packaging options are available to suit your needs:

  • Single kits
  • SixPak (6 plates)
  • PharmPak (50 plates)

Ready to Use Quantikine ELISA Kits

Quantikine High Sensitivity Elisa Kits

High Sensitivity Colorimetric Sandwich ELISA Kits

Quantikine High Sensitivity ELISA Kits are developed with greater sensitivity for quantification of low-level targets in your sample. These kits utilize distinct protocols to achieve their impressive levels of analyte detection. Two different amplification systems are available.

QuickKit Elisa Kits

QuicKit™ Colorimetric Sandwich ELISA Kits

QuicKit ELISAs employ a fast, simplified protocol that allows for rapid quantitation of target protein in only 90 minutes. These ready-to-use kits allow you to accomplish more, while achieving the same legendary quality of a Quantikine ELISA.

Quantikine ELISA Kit Assay Principle

Quantikine Elisa Kit Assay Principle showing steps in a Quantikine sandwich ELISA kit

Step one: A microplate pre-coated with capture antibody is provided. Samples or standards are added and any analyte present is bound by the immobilized antibody.  

Step two: A second HRP-labeled antibody (detection antibody) is added and binds to the captured analyte.  

Step three: A substrate solution is added to the wells and a blue color develops in proportion to the amount of analyte present in the sample. Color development is stopped, turning the color in the wells to yellow. The absorbance of the color at 450 nm is measured.

Featured Application Areas

green immune cell sending out cytokines

Immune Response Profiling

Bio-Techne has a broad selection of immunoassays for immune response profiling. Choose the immunoassay that best suits your needs.


Immuno-Oncology Research

Bio-Techne offers a broad range of sensitive and accurate immunoassays for immuno-oncology research.

Unique Animal Model Systems

Animal Model Systems

With hundreds of immunoassays spanning 19 different species, Bio-Techne can support you with your unique model organism research.

Automated ELISA systems Ella runs single, multianalyte and multiplex immunoassays

Analytical Solutions for Cell and Gene Therapy

Rigorously characterize your products for the safest possible therapies. Our platforms enable rapid and accurate analysis of protein contaminants and vectors.

Serum CTLA-4  levels in cancer patients measured with Quantikine High Sensitivity CTLA-4 ELISA

CTLA-4: Immunotherapy and Autoimmunity

The R&D Systems CTLA-4 HS ELISA is sensitive, specific, and provides high-quality data so you don’t have to worry about repeating experiments. 

Cytokine release from immune cell 250x250

Type 1 Interferons

 We compare our Type 1 Interferon Quantikine ELISA kits to the competition. The competition fails to meet commonly accepted standards for recovery and linearity.

Recognition Awards