Recombinant Human GFR alpha-1 Fc Chimera Protein, CF

Name: Recombinant Human GFR alpha-1 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 714-GR

Catalog # 714-GR | R&D Systems, Inc. a Bio-Techne Brand

Citations (11)

Product Datasheet / COA / SDS

Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	NP_665736
Structure / Form:	Disulfide-linked homodimer
Applications:	Binding Activity

View Full Technical Details for Recombinant Human GFR alpha-1 Fc Chimera Protein, CF

Technical Data
Citations (11) / Reviews

Product Specifications for Recombinant Human GFR alpha-1 Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived human GFR alpha-1/GDNF R alpha-1 protein

Human GFR alpha -1 (Ala19-Lys429) Accession # NP_665736	IEGRMD	Human IgG₁ (Pro100-Lys330)	6-His tag
N-terminus			C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ala19

Predicted Molecular Mass

73.3 kDa (monomer)

SDS-PAGE

73.3 kDa (monomer)

Activity

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human GDNF (Catalog # 212-GD) at 1 µg/mL binds Recombinant Human GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera with an apparent K_d <2 nM.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

714-GR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GFR alpha-1/GDNF R alpha-1

Glial cell line-derived growth factor (GDNF), neurturin (NTN), artemin and persephin are distant members of the TGF-beta superfamily. They function as neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits [GDNF receptor alpha -1 (GFR alpha -1) or GFR alpha -2]. GFR alpha -1 and -2 are members of a family of at least four cysteine-rich glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF to membrane‑associated GFR alpha -1 or GFR alpha -2 initiates the association with and activation of the Ret tyrosine kinase. Soluble GFR alpha s released enzymatically from the cell surface-associated protein with phosphatidylinositol phospholipase C, as well as recombinantly produced soluble GFR alpha -1, can also bind with high-affinity to GDNF and trigger the activation of Ret tyrosine kinase.

Human GFR alpha -1 cDNA encodes a 465 amino acid (aa) residue protein with an N-terminal 24 aa residue hydrophobic signal peptide. Like other GPI-linked proteins, human GFR alpha -1 has a C-terminal hydrophobic region which is preceded by a three aa residue (ASS) GPI-binding site. Human GFR alpha -1 shares 93% aa identity with rat GFR alpha -1. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors.

Item	Value
References	Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117. Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47. Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.
Long Name	Glial Cell line-derived Neurotrophic Factor Receptor alpha 1
Entrez Gene IDs	2674 (Human); 14585 (Mouse); 25454 (Rat)
Alternate Names	DKFZp313E1012, DKFZp686J0156, GDNF R alpha-1, GDNF family receptor alpha 1, GDNF family receptor alpha-1, GDNF receptor alpha-1, GDNFR-alpha-1, GDNFRAGFR-ALPHA-1, GDNFRMGC23045, GFR alpha-1, GFR alpha1, GFR-alpha-1, GFRA1, GFRa-1, GPI-linked anchor protein, Glial cell line-derived neurotrophic factor receptor alpha, PI-linked cell-surface accessory protein, RET ligand 1, RET1L, RETL1FLJ10561, TGF-beta related neurotrophic factor receptor 1, TGF-beta-related neurotrophic factor receptor 1, TRNR1FLJ31546

Citations for Recombinant Human GFR alpha-1 Fc Chimera Protein, CF (11)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human GFR alpha-1 Fc Chimera Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

ER Rhymes et al. (2022-07-07), Bimodal regulation of axonal transport by the GDNF-RET signalling axis in healthy and diseased motor neurons Cell Death Disease, 2022-07-07;13(7):584.
PMID: 35798698
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

A Ingles-Pri et al. (2021-04-15), Optogenetic delivery of trophic signals in a genetic model of Parkinson's disease PloS Genetics, 2021-017(4):e1009479.
PMID: 33857132
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

K Ban et al. (2017-05-10), RET signaling in prostate cancer Clin. Cancer Res., 2017-00(0).
PMID: 28490466
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Meka D et al. (2015-03-30), Parkin cooperates with GDNF/RET signaling to prevent dopaminergic neuron degeneration. J Clin Invest, 2015-0125(5):1873-85.
PMID: 25822020
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Klein P et al. (2014-01-28), Ret rescues mitochondrial morphology and muscle degeneration of Drosophila Pink1 mutants. EMBO J, 2014-033(4):341-55.
PMID: 24473149
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Gatto G et al. (2013-03-20), Protein tyrosine phosphatase receptor type O inhibits trigeminal axon growth and branching by repressing TrkB and Ret signaling. J Neurosci, 2013-033(12):5399-410.
PMID: 23516305
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Lundgren TK et al. (2012-02-15), RET PLC-gamma phosphotyrosine binding domain regulates Ca2+ signaling and neocortical neuronal migration. PLoS ONE, 2012-07(2):e31258.
PMID: 22355350
Species: Bacteria
Sample Types: Trypanosoma
Applications: PcIP

Lu B et al. (2008-06-09), A novel immunoprecipitation strategy identifies a unique functional mimic of the glial cell line-derived neurotrophic factor family ligands in the pathogen Trypanosoma cruzi. Infect. Immun., 2008-076(8):3530-8.
PMID: 18541656
Species: Chicken
Sample Types: Whole Tissue
Applications: Bioassay

Tatsumi N et al. (2007-04-03), Neurturin-GFRalpha2 signaling controls liver bud migration along the ductus venosus in the chick embryo. Dev. Biol., 2007-0307(1):14-28.
PMID: 17509555
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Murase S et al. (2006-09-20), A specific survival response in dopamine neurons at most risk in Parkinson's disease. J. Neurosci., 2006-026(38):9750-60.
PMID: 16988046
Species: Rat
Sample Types: Recombinant Protein, Whole Cells
Applications: Bioassay, ELISA Developmet, Surface Plasmon Resonance

Carmillo P et al. (2005-02-22), Glial cell line-derived neurotrophic factor (GDNF) receptor alpha-1 (GFR alpha 1) is highly selective for GDNF versus artemin. Biochemistry, 2005-044(7):2545-54.
PMID: 15709767

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