Does IL-1 beta induce NO in smooth muscle?

Name: Recombinant Human IL-1 beta/IL-1F2, Animal-Free Protein
Brand: R&D Systems
SKU: AFL201

Yes it does, based on the literature.

Recombinant Human IL-1 beta/IL-1F2, Animal-Free Protein

Catalog # AFL201 | R&D Systems, Inc. a Bio-Techne Brand

Citations (1)

Product Datasheet / COA / SDS

Intended for preclinical researchers who may transition to GMP IL-1beta for their clinical work

Setting the standard in quality research reagents

Key Product Details

Learn more about Animal-Free Recombinant Proteins

Source:	E. coli
Accession #:	NP_000567.1
Applications:	Bioactivity

View Full Technical Details for Recombinant Human IL-1 beta/IL-1F2, Animal-Free Protein

Technical Data
Citations (1) / Reviews / FAQs

Product Specifications for Recombinant Human IL-1 beta/IL-1F2, Animal-Free Protein

Source	E. coli-derived human IL-1 beta/IL-1F2 protein Ala117-Ser269 Produced using non-animal reagents in an animal-free laboratory.
Purity	>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala117 & Pro118
Predicted Molecular Mass	17 kDa
SDS-PAGE	17 kDa
Activity	Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED₅₀ for this effect is <12 pg/mL. The specific activity of Recombinant Human IL-1 beta is >5.0 x 10⁷ IU/mg, which is calibrated against recombinant human IL-1 beta WHO International Standard (NIBSC code: 86/680).

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AFL201

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with trehalose.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 12 months, 2 to 8 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL‑1 R3/IL‑1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1‑4). The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5‑7). The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%‑78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL‑1 beta.

Item	Value
References	Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21. March, C.J. et al. (1985) Nature 315:641. Auron, P.E. et al. (1984) Proc. Natl. Acad. Sci. 81:7907. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.
Long Name	Interleukin 1 beta
Entrez Gene IDs	3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Alternate Names	IL-1, IL-1 beta, IL-1F2, IL-1b, IL1 beta, IL1-BETA, IL1B, IL1F2IL-1 beta, catabolin, interleukin 1, beta, interleukin-1 beta, preinterleukin 1 beta, pro-interleukin-1-beta

Manufacturing Specifications

Animal-Free Manufacturing Conditions
Our dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal components or byproducts. Every stage of manufacturing is conducted in compliance with R&D Systems' stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.

Production

All molecular biology procedures use animal-free media and dedicated labware.
Dedicated fermentors are utilized in committed animal-free areas.

Purification

Protein purification columns are animal-free.
Bulk proteins are filtered using animal-free filters.
Purified proteins are stored in animal-free containers in a dedicated cold storage room.

Quality Assurance

Low Endotoxin Level.
No impairment of biological activity.
High quality product obtained under stringent conditions.
For ex vivo research or bioproduction, additional documentation can be provided.

Please read our complete Animal-Free Statement

Product Specific Notices for Recombinant Human IL-1 beta/IL-1F2, Animal-Free Protein

This product is covered by one or more of the following U.S. patents: 5,681,933 and 5,474,899.

Citations for Recombinant Human IL-1 beta/IL-1F2, Animal-Free Protein (1)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human IL-1 beta/IL-1F2, Animal-Free Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Souheil-Antoine Younes (2016-06-20), IL-15 promotes activation and expansion of CD8+ T cells in HIV-1 infection J Clin Invest, 2016-0126(7):2745-2756.
PMID: 27322062

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FAQs for Recombinant Human IL-1 beta/IL-1F2, Animal-Free Protein

Does IL-1 beta induce NO in smooth muscle?

Yes it does, based on the literature.