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Recombinant Human Wnt-3a Protein Best Seller

Catalog # 5036-WN | R&D Systems, Inc. a Bio-Techne Brand
Recombinant Human Wnt-3a Protein Bioactivity
(4)
MAB2105; green) and a Goat Anti-Human/Mouse Desmin Antigen Affinity-purified Polyclonal Antibody (<a class="NoLineLink" href=https://www.bio-techne.com/p/proteins-enzymes/"https://www.bio-techne.com/p/antibodies/human-mouse-desmin-antibody_af3844" target="_self">AF3844</a>; red) to visualize myofibroblast cells and counterstained with DAPI (<a class="NoLineLink" href=https://www.bio-techne.com/p/proteins-enzymes/"https://www.bio-techne.com/p/fluorescent-probes-dyes/dapi_5748" target="_self">5748</a>; blue). (B) Human intestinal organoids were stained using a Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (<a class="NoLineLink" href=https://www.bio-techne.com/p/proteins-enzymes/"https://www.bio-techne.com/p/antibodies/human-mouse-e-cadherin-antibody_af748" target="_self">AF748</a>; green) and a Mouse Anti-Human MUC2 Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-44431; red) and counterstained with DAPI (<a class="NoLineLink" href=https://www.bio-techne.com/p/proteins-enzymes/"https://www.bio-techne.com/p/fluorescent-probes-dyes/dapi_5748" target="_self">5748</a>; blue)." data-badges="" data-application="Cell Culture" width="250" height="250" class="search_result_thumbnail_image" />
AF748; red) and DAPI (<a class="NoLineLink" href=https://www.bio-techne.com/p/proteins-enzymes/"https://www.bio-techne.com/p/fluorescent-probes-dyes/dapi_5748" target="_self">5748</a>; blue). The image shown was taken at 10x magnification. (B) Organoids were fixed and stained with a Mouse Anti-Human Chromogranin A Monoclonal Antibody (<a class="NoLineLink" href=https://www.bio-techne.com/p/proteins-enzymes/"https://www.bio-techne.com/p/antibodies/human-chromogranin-a-antibody-948128_mab90981" target="_self">MAB90981</a>; green) to visualize enteroendocrine cells and counterstained with a Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (<a class="NoLineLink" href=https://www.bio-techne.com/p/proteins-enzymes/"https://www.bio-techne.com/p/antibodies/human-mouse-e-cadherin-antibody_af748" target="_self">AF748</a>; red) and DAPI (<a class="NoLineLink" href=https://www.bio-techne.com/p/proteins-enzymes/"https://www.bio-techne.com/p/fluorescent-probes-dyes/dapi_5748" target="_self">5748</a>; blue). The image shown was taken at 20x magnification." data-badges="" data-application="Cell Culture" width="250" height="250" class="search_result_thumbnail_image" />
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5036-WN-010/CF
5036-WN-500/CF

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5036-WN-010
5036-WN-500
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Setting the standard in quality research reagents

Key Product Details

Source: CHO
Accession #: NP_149122
Applications: Bioactivity

Citations for Recombinant Human Wnt-3a Protein (334)

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Customer Reviews for Recombinant Human Wnt-3a Protein (27)

4.7 out of 5
27 customer ratings
5 stars
78%
4 stars
15%
3 stars
7%
2 stars
0%
1 star
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  • Recombinant Human Wnt-3a Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 10/11/2023
    Recombinant Human Wnt-3a Protein In vitro bioactivity in cell culture 5036-WN
  • Recombinant Human Wnt-3a Protein
    Name: MARIA PINNA
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 09/22/2023
  • Recombinant Human Wnt-3a Protein
    Name: Anonymous
    Application: Cell migration/motility
    Verified Customer | Posted 05/20/2023
    wnt3a promote wound healing.
    Recombinant Human Wnt-3a Protein Cell migration/motility 5036-WN
  • Recombinant Human Wnt-3a Protein
    Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 01/01/2022
    wnt3a was used for the differentiation of cerebral organoid
    Recombinant Human Wnt-3a Protein Stem/Immune cell maintenance or differentiation  5036-WN
  • Recombinant Human Wnt-3a Protein
    Name: Anonymous
    Application: CellProlif
    Verified Customer | Posted 11/29/2021
    avoid freezing and refreezing.
    Recombinant Human Wnt-3a Protein Cell Proliferation 5036-WN
  • Recombinant Human Wnt-3a Protein
    Name: Ricardo Malvicini
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 09/26/2021
  • Recombinant Human Wnt-3a Protein
    Name: Anonymous
    Application: CellProlif
    Verified Customer | Posted 08/17/2021
    200ng/ml wn3a CF can further promote the colony formation efficiency.
    Recombinant Human Wnt-3a Protein Cell Proliferation 5036-WN
  • Recombinant Human Wnt-3a Protein
    Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 08/17/2021
    Recombinant Human Wnt-3a Protein Stem/Immune cell maintenance or differentiation  5036-WN
  • Recombinant Human Wnt-3a Protein
    Name: Mike van Nijmegen
    Application: Enzymatic activity in vitro
    Reason for Rating: excellent product, very repeated product, we bought it many times with different lot numbers
    Verified Customer | Posted 08/06/2021
    Recombinant Human Wnt-3a Protein Enzymatic activity in vitro 5036-WN
  • Recombinant Human Wnt-3a Protein
    Name: Mike van Nijmegen
    Application: CellProlif
    Reason for Rating: This product works good.
    Verified Customer | Posted 07/20/2021
    Use the final concentration that is recommended, don't make the final concentration very low. Hek293SFT cells were treated with different concentrations of wnt3a, and topflash assay was performed to detect the luciferase activity of wnt3a.
    Recombinant Human Wnt-3a Protein Cell Proliferation 5036-WN
  • Recombinant Human Wnt-3a Protein
    Name: Ermali Aliaj
    Application: Cell Culture
    Verified Customer | Posted 01/02/2021
  • Recombinant Human Wnt-3a Protein
    Name: Techung Lee
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 11/06/2020
    HepG2 cells were treated with 100 ng/ml Wnt3a for 2 days, following which the LDH activity of cell culture medium samples were assayed. The result shows that Wnt3a increased LDH release in HepG2 cells.
    Recombinant Human Wnt-3a Protein Enzymatic activity in vitro 5036-WN
  • Recombinant Human Wnt-3a Protein
    Name: Anonymous
    Application: SDS-PAGE Control
    Reason for Rating: Recombinant Wnt3a product was added to activate Wnt/ beta -catenin signaling pathway.
    Verified Customer | Posted 02/16/2020
    Recombinant Human Wnt-3a Protein SDS-PAGE Control 5036-WN
  • Recombinant Human Wnt-3a Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Reason for Rating: This protein (200 ng/mL) works well for activating Wnt signaling as shown by Topflash luciferase activity.
    Verified Customer | Posted 12/16/2018
    Recombinant Human Wnt-3a Protein In vitro bioactivity in cell culture 5036-WN
  • Name: Mark Esposito
    Application: In vitro bioactivity in cell culture
    Reason for Rating: The product worked to activate Wnt signaling in breast cancer cells with a Wnt reporter, however performed less well than supernatants from L cell Wnt3a lines.
    Verified Customer | Posted 10/27/2018
    Recombinant Wnt3a product was added at 100 ng/mL for 2 days on 7TGC reporter cell lines. Activation was measured with GFP levels by Flow.
    Recombinant Human Wnt-3a Protein In vitro bioactivity in cell culture 5036-WN
  • Name: Anonymous
    Application: CellProlif
    Verified Customer | Posted 10/17/2018
  • Recombinant Human Wnt-3a Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 12/06/2017
    Recombinant Human Wnt-3a Protein In vitro bioactivity in cell culture 5036-WN
  • Recombinant Human Wnt-3a Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 06/07/2017
    Recombinant Human Wnt-3a Protein In vitro bioactivity in cell culture 5036-WN
  • Recombinant Human Wnt-3a Protein
    Name: Anonymous
    Application: Cell migration/motility
    Verified Customer | Posted 03/14/2017
    Cell motility assay performed with MA-11 cells untreated (A) and treated (B) for 72 hours with R&D Recombinant Human Wnt-3a (#5036-WN/CF) at 250 ng/mL.
    Recombinant Human Wnt-3a Protein Cell migration/motility 5036-WN
  • Recombinant Human Wnt-3a Protein
    Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 12/15/2016
    We use for stem cell maintenance and differentiation.
  • Recombinant Human Wnt-3a Protein
    Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 12/15/2016
    We use this product for our neuron stem cells studies.
  • Recombinant Human Wnt-3a Protein
    Name: Francesco Marino
    Application: In vitro bioactivity in cell culture
    Reason for Rating: It works well.
    Verified Customer | Posted 11/11/2016
    I tested the activity of the recombinant protein in the HEK293 Top Flash Reporter cell lines. LiCl was used as a positive control.
    Recombinant Human Wnt-3a Protein In vitro bioactivity in cell culture 5036-WN
  • Recombinant Human Wnt-3a Protein
    Name: Anonymous
    Application: Luciferase reporter activity
    Verified Customer | Posted 08/08/2016
    Luciferase reporter activity by TOPFlash/FOPFlash for Wnt/B-catenin activity was performed in cultured cells. Cells were transfected for 48 hours with plasmid and treated with Wnt3a for 24 hours prior to performing Dual-Glo luciferase assay.
    Recombinant Human Wnt-3a Protein Other 5036-WN
  • Recombinant Human Wnt-3a Protein
    Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Reason for Rating: We attempted to use the product to induce skeletal muscle differentiation in Mus musculus stem cells, but our results suggest differentiation to cardiomyocytes.
    Verified Customer | Posted 05/13/2016
    Recombinant Human Wnt-3a Protein Stem/Immune cell maintenance or differentiation  5036-WN
  • Recombinant Human Wnt-3a Protein
    Name: Shireen Jayman
    Application: CellProlif
    Verified Customer | Posted 01/15/2016
  • Name: Anonymous
    Application: SDS-PAGE Control
    Reason for Rating: I have used Wnt3a routinely to stimulate primary cells in culture. I have obtained consistent results with reproducible data.
    Verified Customer | Posted 01/13/2016
  • Recombinant Human Wnt-3a Protein
    Name: Anonymous
    Application: Media additive for protein or antibody production
    Reason for Rating: This protein works very well for activating Wnt signaling as shown by B-catenin expression.
    Verified Customer | Posted 01/13/2016

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FAQs for Recombinant Human Wnt-3a Protein

  • Can I reconstitute Recombinant Human Wnt-3a Protein (catalog # 5036-WN) at a different concentration than recommended?  

    We cannot guarantee the performance if the protein is reconstituted at a different concentration than recommended in the product datasheet. Please contact Technical Service if you have any questions or concerns about reconstitution.

  • Can I reconstitute Recombinant Human Wnt-3a Protein (catalog # 5036-WN) in a different buffer than recommended?

    For best recovery, stability, and activity, the protein should be reconstituted in the buffer recommended on the product datasheet. Stability testing has been evaluated under these conditions; we can support the performance under these reconstitution conditions.

Product Documents for Recombinant Human Wnt-3a Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.