SARS-CoV-1/2 Spike RBD Llamabody Antibody
Catalog # LMAB10541 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Summary for SARS-CoV-1/2 Spike RBD Llamabody Antibody
Immunogen
Accession # YP_009724390
Specificity
Clonality
Host
Isotype
Scientific Data Images for SARS-CoV-1/2 Spike RBD Llamabody Antibody
Specificity of Anti-SARS-CoV-1/2 Spike RBD (Clone VHH72) Llamabody in Direct ELISA.
A panel of SAR-CoV-2 Spike and RBD proteins were coated at 2 μg/mL in 96-well polystyrene plates. Anti-SARS-CoV-1/2 RBD (Clone VHH72) Llamabody (Catalog # LMAB10541, 7.81 ng/mL) detected SARS-CoV-2 Spike Protein S1 and SARS CoV-2 Spike Protein RBD. Anti-SARS RBD Clone CR3022 was included as a positive control for detection of SARS-CoV-2 Spike Protein S1 and SARS CoV-2 Spike Protein RBD. No cross-reactivity with SARS-CoV-2 Spike Protein S2 was detected.SARS-CoV-2 Spike RBD Binding to ACE-2-transfected HEK293 Human Cell Line is Blocked by SARS-Cov-1/2 Spike RBD Llamabody.
In a functional flow cytometry test, His-tagged recombinant SARS-CoV-2 Spike RBD (50 ng/mL, 10534-CV) binds to HEK293 human embryonic kidney cell line transfected with human ACE-2 (black dotted line). Binding is completely blocked (orange histogram) by 25 µg/mL of Anti-SARS CoV-1/2 S1 RBD Llamabody (Catalog # LMAB10541). Mouse anti-Human DC-SIGN Monoclonal Antibody (MAB161) at 25 µg/mL was used as an irrelevant control (blue line). Cells were stained with Allophycocyanin-conjugated Mouse anti-His tag Monoclonal Antibody (IC050A).Applications for SARS-CoV-1/2 Spike RBD Llamabody Antibody
Blockade of Receptor-ligand Interaction
ELISA
Formulation, Preparation and Storage
Purification
Endotoxin Level
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Spike RBD
References
- Wu, F. et al. (2020) Nature 579:265.
- Tortorici, M.A. and D. Veesler (2019). Adv. Virus Res. 105:93.
- Bosch, B.J. et al. (2003) J. Virol. 77:8801.
- Belouzard, S. et al. (2009) Proc. Natl. Acad. Sci. 106:5871.
- Millet, J.K. and G. R. Whittaker (2015) Virus Res. 202:120.
- Yuan, Y. et al. (2017) Nat. Commun. 8:15092.
- Walls, A.C. et al. (2010) Cell 180:281.
- Jiang, S. et al. (2020) Trends. Immunol. https://doi.org/10.1016/j.it.2020.03.007.
- Ortega, J.T. et al. (2020) EXCLI J. 19:410.
- Wrapp, D. et al. (2020) Science 367:1260.
- Tai, W. et al. (2020) Cell. Mol. Immunol. https://doi.org/10.1016/j.it.2020.03.007.
- Okba, N. M. A. et al. (2020). Emerg. Infect. Dis. https://doi.org/10.3201/eid2607.200841.
- Wang, X. et al. (2020) https://doi.org/10.1038/s41423-020-0424-9.
- Wang, K. et al. (2020) bioRxiv https://www.biorxiv.org/content/10.1101/2020.03.14.988345v1.
Long Name
Alternate Names
Gene Symbol
UniProt
Product Documents for SARS-CoV-1/2 Spike RBD Llamabody Antibody
Product Specific Notices for SARS-CoV-1/2 Spike RBD Llamabody Antibody
For research use only
FAQs for SARS-CoV-1/2 Spike RBD Llamabody Antibody
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Q: What is the Isoelectricpoint (pI) of SARS-CoV-1/2 Spike RBD Llamabody VHH MAb (Clone VHH72), Catalog # LMAB10541?
A: The pI of SARS-CoV-1/2 Spike RBD Llamabody VHH MAb (Cl VHH72), Catalog # LMAB10541, is 6.35 for the primary species. It is not unexpected to observe a range of pI from 6.1 to 6.9 due to some variability in glycosylation.
Reconstitution Calculator
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