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Rapid Characterization of Immune Response to SARS-CoV-2 Antigens

Multi SARS-CoV-2 Antigen Serological Assay for Jess & Abby

The SARS-CoV-2 Multi-Antigen Serology Module (SA-001) for use with Jess™ and Abby™ automated Simple Western™ systems enables the detection of human IgG antibodies in human serum or plasma reactive against multiple SARS-CoV-2 antigens.

  • Quantify differences in human antibody binding using five SARS-CoV-2 antigens in each sample.
  • Minimal set-up time, hands-free operation, and fully analyzed results on 24 samples in 3 hours.
  • Access your results remotely to minimize time in the lab for social distancing.
SARS CoV-2 Multi Antigen Serology Module Kit

The Simple Western SARS-CoV-2 serological assay is validated for human serum and plasma. Proof-of-concept studies have also shown that the SARS-CoV-2 Antigen Serological Assay can be used to monitor the COVID-19 immune response in animal systems such as mice when the appropriate secondary antibody is chosen.

Figure 1. With this antigen down serological assay, you can quantitatively characterize serum or plasma human IgG antibodies reactive against recombinant Nucleocapsid Protein (N), S1 receptor binding domain protein (RBD), S1 subunit full length, S2 subunit full length, and Spike (S1+S2) viral antigens within one 3-hour run!

Simple Western Vaccine Multiantigen Serology graph

The SARS-CoV-2 Multi-Antigen Serology Module for Simple Western provides an immune fingerprint of serum antibodies against five viral antigens of interest for characterization of the COVID-19 immune response in only three hours. As little as 1 µL of serum can be utilized for each patient sample.

Figure 2. The Simple Western SARS-CoV-2 serological assay demonstrates differential human serum reactivity to N, S1 RBD, S1 full length, S2 full length, and Spike (S1+S2) COVID-19 viral antigens by quantification of percent peak area for SARS-CoV-2 antigens detected by PCR positive human serum.


Multi SARS-CoV-2 Antigen Serological Assay data set

I Need a Fast, Confirmatory Serological Assay for My SARS-CoV-2 ELISA

Western blot-based serological assays are commonly used to confirm ELISA-based serology tests. The SARS-CoV-2 Multi-Antigen Serology Module for Simple Western demonstrates excellent agreement with the recently released COVID-SeroIndex™ Kantaro Quantitative SARS-CoV-2 IgG Antibody RUO ELISA (97% positive percent agreement and 99% negative percent agreement). The fully automated Simple Western serological assay is a powerful orthogonal assay that provides characterization of human immune responses to all five SARS-CoV-2 antigens in only three hours. 

Positive Percent Agreement and Negative Percent Agreement study benchmarking the SARS-CoV-2 Multi-Antigen Serology Module against the Kantaro COVID-SeroIndex™ RUO ELISA.

Benchmarking Against the Kantaro COVID-SeroIndex™ RUO ELISA
    Kantaro COVID-SeroIndex™ RUO ELISA
Positive Negative


SARS-CoV-2 Multi-Antigen Serology Module

Positive 64 1
Negative 2 99
  Total 66 100
  Positive Percent Agreement 97%  
  Negative Percent Agreement 99%