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Simple Plex™ Assays for Viral Titer Quantification
Fast and reproducible viral capsid measurement is essential for those focused on gene transfer, as part of a cell and gene therapy workflow. While traditional plate-based and dot blot assays can be useful, they are often complex, time-consuming, and non-standardized. See how Simple Plex assays on the EllaTM platform are used for viral titer quantification. Next, meet Ella and request information. Finally, choose the Simple Plex Assay that best meets your needs.
Table of Contents
- Webinar: Scaling Analytics for Cell and Gene Therapy With Ella
- Comparing the Simple Plex AAV Assays With the PROGEN AAV ELISAs
- Select A Simple Plex AAV Assay: AAV1, AAV2, AAV6, and AAV8
- Lentiviral Vector Titration
- Find Out About Bio-Techne's Tools For Cell And Gene Therapy
- View Complete Simple Plex Assay Menu
Automated Simple Plex assays on the Ella platform offer a fast, sensitive, and reproducible method for reliable viral capsid measurement. Simple Plex assays are available to quantify the viral titer of both lentivirus and adeno-associated virus capsids.
- Measure viral titer across a broad dynamic range
- Get triplicate data results with just 25 µL or less of sample volume
- Streamline workflow with only 15 minutes of hands-on assay prep time
- Achieve the same reproducible answers across multiple users and multiple sites
- Get highly reproducible validated data with no manual steps in just 90 minutes
Scaling Analytics for Cell and Gene Therapy With Ella
Watch how Dr. Kiren Baines, a bioprocessing scientist from eXmoor, uses Ella to scale analytics for AAV process development.
Fast and Reproducible AAV Vector Titration
To meet increased throughput and safety demands, investigators are seeking more automated analytical solutions for assessment of viral vector identity, purity, and stability. Simple Plex AAV assays on the Ella platform provide an accurate and efficient method for measuring the AAV capsid titer. The assay uses the recombinant AAV antibodies from industry leader PROGEN to quantify assembled AAV capsids.
Combining the proven specificity of PROGEN AAV antibodies with the convenient workflow and robustness of the Ella platform, Simple Plex AAV viral titer assays:
- Offer a broad viral detection range combined with hands-free automation to accelerate AAV viral capsid quantification
- Use factory calibrated assay cartridges, which minimize setup time and reduce user-dependent variability while reducing the need for sample retests
- Rely on the innovative Ella platform to perform every step of the immunoassay automatically, from wash steps to final
- Provide a robust alternative to the conventional ELISA or dot blot approaches
- Deliver superior assay sensitivity, helping to ensure data quality and consistency
Comparing Simple Plex AAV Assays With the PROGEN AAV ELISAs
In this application note, we demonstrate assay comparability between the Simple Plex AAV assays and the PROGEN AAV Titration ELISAs to show that you get the same high-quality data you would expect from the PROGEN ELISA with an automated, streamlined workflow.
Creating Lentiviral Vectors With Fast, Reliable Viral Titration
With the growing use of lentiviral vectors derived from HIV-1 in gene transfer workflows, researchers need fast, reliable viral titer quantification during process development. The 3-to-6-hour timeframe required to run a standard ELISA is a challenge for operators who increasingly require results quickly to inform real-time decision-making.
The Simple Plex assay for the detection of human HIV-1 Gag p24 combines high throughput with superior accuracy and precision to enable rapid quantification of lentiviral capsid proteins throughout different stages of cell and vector manufacturing. The automated, reproducible nature of the Simple Plex p24 assay helps eliminate user error, making it an ideal tool in good manufacturing practice (GMP) environments where quality, standardization of process, assay transfer, and scalability are highly desirable.
Bio-Techne's Tools For Cell And Gene Therapy
Bio-Techne offers a diverse portfolio of products and services designed to accelerate and simplify your cell and gene therapy workflow. From GMP proteins and small molecules to state-of-the-art analytical instrumentation, Bio-Techne is committed to providing high quality innovative solutions. Learn more about Bio-Techne’s cell and gene therapy offerings.
What are viral titer assays?
Viral titer assays are designed to quantify viral vector concentration. In addition to cell and gene therapy gene transfer workflows, viral titer assays are integral to the study of infectious disease pathogenesis and vaccine development. At a high level, viral titer assays quantify: DNA titer, infectious titer, or capsid titer. DNA titer assays quantify the number of viral capsids containing DNA. Infectious titer assays quantify infectious particles that transfer DNA into host cells via a process called viral transduction. Capsid titer assays quantify viral capsids. Capsids may or may not contain viral DNA.
Learn more about viral titer assays at our Partner PROGEN’s website
What type of viral titer assay is the Simple Plex Immunoassay?
Simple Plex automated viral titer assays quantify viral particles, also known as capsids.
Learn more about Simple Plex Assays and the Ella Platform
I am looking for automated and scalable solutions for cell and gene therapy research and manufacturing. What do you suggest?
ProteinSimple, a Bio-techne Brand has several analytical solutions to suit your cell and gene therapy research and development needs. Download our eBook to see real-world examples of time, cost, and sample savings from our analytical solutions.
Download the Next Generation Analytical Solutions for Cell and Gene Therapy eBook
Do you have any other resources for cell and gene therapy research and manufacturing?
Bio-Techne spans the cell and gene therapy workflow. Solutions are available for immune cell therapy and regenerative medicine. Gene engineering services are also available using our nonviral TC Buster™ gene delivery system. GMP-grade raw materials and services are also available.
Find out more about our solutions for cell and gene therapy research and manufacturing at Bio-Techne.
Simple Plex Applications
COVID-19 Research
COVID-19 Research
Quantify cytokine and pro-inflammatory responses to SARS-CoV-2 infection in 90 minutes using an automated, multianalyte format.
Inflammation Research
Inflammation Research
Simple Plex assays enable rapid and reproducible quantification of inflammatory responses such as cytokine release syndrome and sepsis.
Cancer Biomarker Research
Cancer Biomarker Research
Simple Plex assays for cancer research have the sensitivity to profile low abundance cancer biomarkers.
Neuroscience Research
Neuroscience Research
Measure key neuroscience biomarkers such as neurofilament light (NF-L) and neurofilament heavy (NF-H) in CSF, plasma, and serum.
Cell Therapy Research and Development
Cell Therapy Research and Development
The Simple Plex HEK 293 HCP 3G assay on the Ella platform offers broad detection coverage of this host cell protein (HCP).
Bioprocess Testing
Bioprocess Testing
Iterate your workflow faster with Simple Plex CHO HCP 3G-1 assays for process-related impurity testing.
Preclinical Research
Preclinical Research
With a four-log range of detection, Simple Plex assays have the sensitivity to pick up even very low picogram-per-milliliter levels of endogenous mouse or rat analytes.
Viral Titer Assays
Viral Titer Assays
Get highly reproducible adeno-associated virus and lentivirus titer data with no manual steps in just 90 minutes.
Simple Plex Cartridge Denominations
| 16x1 | 32x1 | 16x2 | 16x3 | 16x4 | 32x2 | 72x1 | 72x2 | 32x3 | 32x4 | 32x5 | 32x6 | 32x7 | 32x8 | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Samples | 16 | 32 | 16 | 16 | 16 | 32 | 72 | 72 | 32 | 32 | 32 | 32 | 32 | 32 |
| Plex | 1 | 1 | 2 | 3 | 4 | 2 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 |
| Replicates | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 2 | 3 | 3 | 2 | 2 | 2 | 2 |
| Reportable Results | 16 | 32 | 32 | 48 | 64 | 64 | 72 | 144 | 96 | 128 | 160 | 192 | 224 | 256 |
