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Simple Western™ systems are open platforms, meaning the possibilities are almost endless when selecting antibodies to develop your assays. The Simple Western Antibody database is curated to facilitate the selection process by providing general assay development guidance for identifying and selecting antibodies to test. These guidelines are provided to save time with recommended starting points to optimize for the biological system being examined.
How do antibodies become validated for Simple Western?
- R&D Systems, Novus Biologicals, and valued partners such as Cell Signaling Technology and Bethyl Laboratories are continually validating their antibodies for use with Simple Western.
- Help other scientists and fund your research! Submit new antibody validation data to the Simple Western antibody database and receive a free 8x25 separation module or detection module. Learn more about the Simple Western Antibody Validation Promotion.
- Our expert in-house scientists continue to validate antibodies and review customer-submitted data to ensure the recommendations meet our high standards, so come back for the latest list of validated antibodies in the future.
You can sort by column headings or just type into the Search box. If you don't see your antibody listed here, email us at support@proteinsimple.com.
*Antibodies validated and backed by Cell Signaling Technology (CST)
Simple Western Validated Antibodies
Filter Antibodies: (3371)
| Protein Target | Antibody Type | Vendor / Cat # | Protein Isoform | Citations & Supporting Data | Host Species | Sample | Separation | Dilution | kDa on Simple Western |
|---|---|---|---|---|---|---|---|---|---|
| α-actinin | Primary |
Santa Cruz Biotechnology sc-17829 |
Unmodified |
Citations
(1) Datasheet |
Mouse Monoclonal | NCI-H1975 | Size | Not_Stated | Not_Stated |
| α-Cardiac Actin | Primary |
R&D Systems MAB9308 |
alpha-Cardiac Actin | Datasheet | Mouse Monoclonal | Human, mouse, and rat heart tissue | Size | 2 µg/mL | 50 |
| α-Nucleocapsid | Primary |
Novus Biologicals NB100-56683 |
SARS Nucleocapsid Protein | Datasheet | Rabbit Polyclonal | Recombinant SARS-CoV-2 Nucleocapsid Protein (Novus Biologicals PN 10474-CV); Lysates of SARS-CoV-2) | Size | 25 mg/mL | 60 |
| α-Synuclein | Primary |
Cell Signaling Technology 2642 |
(α) alpha-Synuclein | Rabbit Polyclonal | Size | 1:25 | 21 | ||
| α-Synuclein | Primary |
Cell Signaling Technology 2647 |
(α) alpha-Synuclein (Syn204) | Mouse Monoclonal | Spinal cord 3 ug/ul | Size | 1:10 | 22 | |
| α-Synuclein | Primary |
Cell Signaling Technology 2647 |
(α) alpha-Synuclein (Syn204) | Mouse Monoclonal | Spinal cord 3 ug/uL | Size | 1:10 | 22 | |
| α-Synuclein, Phospho | Primary |
Cell Signaling Technology 23706 |
Phospho (α) alpha-Synuclein (Ser129) (D1R1R) | Rabbit Monoclonal | Spinal cord 3 ug/ul | Size | 1:250 | 23 | |
| α-Synuclein, Phospho | Primary |
Cell Signaling Technology 93706 |
Phospho (α) alpha-Synuclein (Ser129) (D1R1R) | Rabbit Monoclonal | Spinal cord 3 ug/uL | Size | 1:250 | 23 | |
| α-Tubulin | Primary |
MilliporeSigma T8203 |
alpha Tubulin | Mouse Monoclonal | Transfected HeLa, Human Primary Fibroblast Lysates | Size | 1:100 | ||
| β-Actin | Primary |
Cell Signaling Technology 4967 |
β-Actin | Datasheet | Rabbit Polyclonal | HeLa | Size | 1:10 - 1:50 | 48 |
| β-Actin | Primary |
MilliporeSigma A5411 |
(β) beta-actin | Mouse Monoclonal | Rat Brain Lysate (0.2 mg/mL) | Size | 1:400 | 48 | |
| β-actin | Primary |
MilliporeSigma A5441 |
(β) beta-actin | Mouse Monoclonal | Rat Brain Lysate (0.2 mg/mL) | Size | 1:400 | 48 | |
| β-Actin | Primary |
GeneTex GTX101794 |
Actin Gamma 1 | Rabbit Polyclonal | Human Stomach Smooth Muscles | Size | 1:100 | 44 | |
| β-Actin | Primary |
MilliporeSigma A5316 |
beta-actin | Mouse Monoclonal | Transfected HeLa, Human Primary Fibroblast Lysates | Size | 1:100 | ||
| β-Actin (13E5) | Primary |
Cell Signaling Technology 4970 |
β-Actin (13E5) | Datasheet | Rabbit Monoclonal | HeLa | Size | 1:10 - 1:50 | 48 |
| β-Actin (8H10D10) | Primary |
Cell Signaling Technology 3700 |
β-Actin (8H10D10) | Datasheet | Mouse Monoclonal | HeLa | Size | 1:10 - 1:50 | 49 |
| β-Actin (D6A8) | Primary |
Cell Signaling Technology 8457 |
Unmodified |
Citations
(4) Datasheet |
Rabbit Monoclonal | CHO | Size | 1:50 | Not_Stated |
| β-Catenin | Primary |
Cell Signaling Technology 8480 |
(β) beta-Catenin | Rabbit Monoclonal | NIH-3T3, A172, U87MG, U251 | Size | 1:250 | 90 | |
| β-catenin | Primary |
Cell Signaling Technology 8480 |
Datasheet | Rabbit Monoclonal | HL60 Cell Lysates | Size | 1:25, 1:100, 1:400 | 85 | |
| β-Catenin | Primary |
Cell Signaling Technology 9562 |
(β) beta-Catenin | Rabbit Polyclonal | HeLa | Size | 1:50 | ||
| β-Catenin (D10A8) | Primary |
Cell Signaling Technology 8480 |
Unmodified |
Citations
(2) Datasheet |
Rabbit Monoclonal | A-172 | Size | 1:250 | Not_Stated |
| β-Catenin, Phospho | Primary |
Cell Signaling Technology 2009 |
(β) beta-Catenin, Phospho S33/37 | Rabbit Polyclonal | NIH-3T3, A172, U87MG, U251 | Size | 1:50 | 92 | |
| β-Catenin, Phospho | Primary |
Cell Signaling Technology 9561 |
(β) beta-Catenin, S33/37/T41 | Rabbit Polyclonal | NIH-3T3, A172, U87MG, U251 | Size | 1:50 | 92 | |
| β-Tubulin | Primary |
Cell Signaling Technology 2125 |
Unmodified |
Citations
(9) Datasheet |
Rabbit Monoclonal | Brain | Size | 1:2000 | Not_Stated |
| β-Tubulin | Primary |
LI-COR 926-42211 |
Unmodified |
Citations
(9) Datasheet |
Rabbit Monoclonal | 4T1 | Size | 1:100 | Not_Stated |
| β-Tubulin | Primary |
Abcam ab15568 |
Human (β) beta Tubulin aa 400 to the C-terminus | Rabbit Polyclonal | Mouse embryonic fibroblasts | Size | 1:50 | ||
| β-Tubulin | Primary |
MilliporeSigma T4026 |
(β) beta-Tubulin | Mouse Monoclonal | Huh7.5.1 | Size | |||
| 14-3-3 γ | Primary |
Novus Biologicals NB100-406 |
14-3-3 gamma (γ) | Datasheet | Mouse Monoclonal | HeLa lysate 1.0 mg/ml | Size | 1:12.5 | 36 |
| 14-3-3 γ | Primary |
Novus Biologicals NBP2-27202 |
14-3-3 gamma (γ) | Datasheet | Rabbit polyclonal | Human Brain lysate 0.5 mg/ml | Size | 1:50 | 33 |
| 14-3-3 γ | Primary |
Novus Biologicals NBP2-27370 |
14-3-3 gamma (γ) | Datasheet | Rabbit Polyclonal | Human Brain lysate 0.5 mg/ml | Size | 1:10 | 34 |
| 14-3-3 ζ | Primary |
R&D Systems MAB2669 |
14-3-3 zeta (ζ) | Datasheet | Mouse Monoclonal | SH-SY5Y human neuroblastoma cell line | Size | 2 µg/mL | 34 |
| 15-PGDH/HPGD | Primary |
R&D Systems AF5660 |
15-PGDH/HPGD | Datasheet | Goat Polyclonal | Human liver tissue | Size | 10 µg/mL | 34 |
| 15-PGDH/HPGD | Primary |
Novus Biologicals NB200-179 |
15-PGDH/HPGD | Datasheet | Rabbit polyclonal | Lovo lysate 0.5 mg/ml | Size | 1:500 | 34 |
| 17 beta-HSD1/HSD17B1 | Primary |
R&D Systems MAB7178 |
17 beta-HSD1/HSD17B1 | Datasheet | Mouse Monoclonal | Human placenta tissue | Size | 2.5 µg/mL | 40 |
| 4-Hydroxynonenal | Primary |
R&D Systems MAB3249 |
4-Hydroxynonenal | Datasheet | Mouse Monoclonal | HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 4-Hydroxynonenal (4-HNE) | Size | 5 µg/mL | |
| 4E-BP1, phospho | Primary |
Cell Signaling Technology 9455 |
Phospho 4E-BP1 (T70) | Rabbit polyclonal | Human Liver Tissue | Charge | |||
| 4EBP1 | Primary |
R&D Systems AF3227 |
4EBP1 | Datasheet | Goat Polyclonal | HeLa human cervical epithelial carcinoma cell line | Size | 5 µg/mL | 26 |
| 4EBP1 | Primary |
MilliporeSigma 07-1416 |
4EBP1 | Rabbit polyclonal | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec | Charge | 1:50-1:200 | ||
| 4EBP1 | Primary |
Epitomics 1557-1 |
4EBP1 | Rabbit monoclonal | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec | Charge | 1:50-1:200 | ||
| 4EBP1 | Primary |
Cell Signaling Technology 4923 |
Non-p4EBP1 (Thr46) | Rabbit monoclonal | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec | Charge | 1:50-1:200 | ||
| 4EBP1 | Primary |
Cell Signaling Technology 9452 |
4EBP1 | Rabbit polyclonal | Caco2 cell lysates | Size | 1:50 | ||
| 4EBP1 | Primary |
Cell Signaling Technology 9644 |
4EBP1 | Rabbit monoclonal | MCF7 lysed in Bicine/CHAPS | Size | 1:50-1:100 | 28-32 | |
| 4EBP1 | Primary |
Cell Signaling Technology 9644 |
4EBP1 | Rabbit polyclonal | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec | Charge | 1:200-1:1000 | ||
| 4EBP1, phospho | Primary |
Epitomics 2334-1 |
p4EBP1 (Thr45) | Rabbit monoclonal | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec | Charge | 1:50-1:200 | ||
| 4EBP1, phospho | Primary |
Cell Signaling Technology 2855 |
p4EBP1 (Thr37/46) | Rabbit monoclonal | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec | Charge | 1:50-1:200 | ||
| 4EBP1, phospho | Primary |
Cell Signaling Technology 9451 |
p4EBP1 (Ser65) | Rabbit polyclonal | K562 -/+ Gleevec lysed in Bicine CHAPS | Size | 1:50 | 27-31 | |
| 4EBP1, phospho | Primary |
Cell Signaling Technology 9451 |
p4EBP1 (Ser65) | Rabbit polyclonal | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec | Charge | 1:50-1:200 | ||
| 4EBP1, phospho | Primary |
Cell Signaling Technology 9456 |
p4EBP1 (Ser65) | Rabbit monoclonal | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec | Charge | 1:50-1:200 | ||
| 4EBP1, phospho | Primary |
Cell Signaling Technology 9459 |
p4EBP1(Thr37/46) | Rabbit polyclonal | K562 -/+ Gleevec lysed in Bicine CHAPS | Size | 1:50 | 27-31 | |
| 4EBP1, phospho | Primary |
Cell Signaling Technology 9459 |
p4EBP1 (Thr37/46) | Rabbit polyclonal | MCF7 lysed in Bicine/CHAPS | Charge | 1:50-1:100 |
