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Simple Western Antibody Database

Find Antibodies Validated for Simple Western

Simple Western™ systems are open platforms, meaning the possibilities are almost endless when selecting antibodies to develop your assays. The Simple Western Antibody database is curated to facilitate the selection process by providing general assay development guidance for identifying and selecting antibodies to test. These guidelines are provided to save time with recommended starting points to optimize for the biological system being examined.

How do antibodies become validated for Simple Western?

  • R&D Systems, Novus Biologicals, and valued partners such as Cell Signaling Technology and Bethyl Laboratories are continually validating their antibodies for use with Simple Western.
  • Help other scientists and fund your research! Submit new antibody validation data to the Simple Western antibody database and receive a free 8x25 separation module or detection module. Learn more about the Simple Western Antibody Validation Promotion.
  • Our expert in-house scientists continue to validate antibodies and review customer-submitted data to ensure the recommendations meet our high standards, so come back for the latest list of validated antibodies in the future.

You can sort by column headings or just type into the Search box. If you don't see your antibody listed here, email us at support@proteinsimple.com.

*Antibodies validated and backed by Cell Signaling Technology (CST)

Simple Western Validated Antibodies


Filter Antibodies: (3371)
Protein Target Antibody Type Vendor / Cat # Protein Isoform Citations & Supporting Data Host Species Sample Separation Dilution kDa on Simple Western
α-actinin Primary Santa Cruz Biotechnology
sc-17829
Unmodified Citations (1)
Datasheet
Mouse Monoclonal NCI-H1975 Size Not_Stated Not_Stated
α-Cardiac Actin Primary R&D Systems
MAB9308
alpha-Cardiac Actin Datasheet Mouse Monoclonal Human, mouse, and rat heart tissue Size 2 µg/mL 50
α-Nucleocapsid Primary Novus Biologicals
NB100-56683
SARS Nucleocapsid Protein Datasheet Rabbit Polyclonal Recombinant SARS-CoV-2 Nucleocapsid Protein (Novus Biologicals PN 10474-CV); Lysates of SARS-CoV-2) Size 25 mg/mL 60
α-Synuclein Primary Cell Signaling Technology
2642
(α) alpha-Synuclein Rabbit Polyclonal Size 1:25 21
α-Synuclein Primary Cell Signaling Technology
2647
(α) alpha-Synuclein (Syn204) Mouse Monoclonal Spinal cord 3 ug/ul Size 1:10 22
α-Synuclein Primary Cell Signaling Technology
2647
(α) alpha-Synuclein (Syn204) Mouse Monoclonal Spinal cord 3 ug/uL Size 1:10 22
α-Synuclein, Phospho Primary Cell Signaling Technology
23706
Phospho (α) alpha-Synuclein (Ser129) (D1R1R) Rabbit Monoclonal Spinal cord 3 ug/ul Size 1:250 23
α-Synuclein, Phospho Primary Cell Signaling Technology
93706
Phospho (α) alpha-Synuclein (Ser129) (D1R1R) Rabbit Monoclonal Spinal cord 3 ug/uL Size 1:250 23
α-Tubulin Primary MilliporeSigma
T8203
alpha Tubulin Mouse Monoclonal Transfected HeLa, Human Primary Fibroblast Lysates Size 1:100
β-Actin Primary Cell Signaling Technology
4967
β-Actin Datasheet Rabbit Polyclonal HeLa Size 1:10 - 1:50 48
β-Actin Primary MilliporeSigma
A5411
(β) beta-actin Mouse Monoclonal Rat Brain Lysate (0.2 mg/mL) Size 1:400 48
β-actin Primary MilliporeSigma
A5441
(β) beta-actin Mouse Monoclonal Rat Brain Lysate (0.2 mg/mL) Size 1:400 48
β-Actin Primary GeneTex
GTX101794
Actin Gamma 1 Rabbit Polyclonal Human Stomach Smooth Muscles Size 1:100 44
β-Actin Primary MilliporeSigma
A5316
beta-actin Mouse Monoclonal Transfected HeLa, Human Primary Fibroblast Lysates Size 1:100
β-Actin (13E5) Primary Cell Signaling Technology
4970
β-Actin (13E5) Datasheet Rabbit Monoclonal HeLa Size 1:10 - 1:50 48
β-Actin (8H10D10) Primary Cell Signaling Technology
3700
β-Actin (8H10D10) Datasheet Mouse Monoclonal HeLa Size 1:10 - 1:50 49
β-Actin (D6A8) Primary Cell Signaling Technology
8457
Unmodified Citations (4)
Datasheet
Rabbit Monoclonal CHO Size 1:50 Not_Stated
β-Catenin Primary Cell Signaling Technology
8480
(β) beta-Catenin Rabbit Monoclonal NIH-3T3, A172, U87MG, U251 Size 1:250 90
β-catenin Primary Cell Signaling Technology
8480
Datasheet Rabbit Monoclonal HL60 Cell Lysates Size 1:25, 1:100, 1:400 85
β-Catenin Primary Cell Signaling Technology
9562
(β) beta-Catenin Rabbit Polyclonal HeLa Size 1:50
β-Catenin (D10A8) Primary Cell Signaling Technology
8480
Unmodified Citations (2)
Datasheet
Rabbit Monoclonal A-172 Size 1:250 Not_Stated
β-Catenin, Phospho Primary Cell Signaling Technology
2009
(β) beta-Catenin, Phospho S33/37 Rabbit Polyclonal NIH-3T3, A172, U87MG, U251 Size 1:50 92
β-Catenin, Phospho Primary Cell Signaling Technology
9561
(β) beta-Catenin, S33/37/T41 Rabbit Polyclonal NIH-3T3, A172, U87MG, U251 Size 1:50 92
β-Tubulin Primary Cell Signaling Technology
2125
Unmodified Citations (9)
Datasheet
Rabbit Monoclonal Brain Size 1:2000 Not_Stated
β-Tubulin Primary LI-COR
926-42211
Unmodified Citations (9)
Datasheet
Rabbit Monoclonal 4T1 Size 1:100 Not_Stated
β-Tubulin Primary Abcam
ab15568
Human (β) beta Tubulin aa 400 to the C-terminus Rabbit Polyclonal Mouse embryonic fibroblasts Size 1:50
β-Tubulin Primary MilliporeSigma
T4026
(β) beta-Tubulin Mouse Monoclonal Huh7.5.1 Size
14-3-3 γ Primary Novus Biologicals
NB100-406
14-3-3 gamma (γ) Datasheet Mouse Monoclonal HeLa lysate 1.0 mg/ml Size 1:12.5 36
14-3-3 γ Primary Novus Biologicals
NBP2-27202
14-3-3 gamma (γ) Datasheet Rabbit polyclonal Human Brain lysate 0.5 mg/ml Size 1:50 33
14-3-3 γ Primary Novus Biologicals
NBP2-27370
14-3-3 gamma (γ) Datasheet Rabbit Polyclonal Human Brain lysate 0.5 mg/ml Size 1:10 34
14-3-3 ζ Primary R&D Systems
MAB2669
14-3-3 zeta (ζ) Datasheet Mouse Monoclonal SH-SY5Y human neuroblastoma cell line Size 2 µg/mL 34
15-PGDH/HPGD Primary R&D Systems
AF5660
15-PGDH/HPGD Datasheet Goat Polyclonal Human liver tissue Size 10 µg/mL 34
15-PGDH/HPGD Primary Novus Biologicals
NB200-179
15-PGDH/HPGD Datasheet Rabbit polyclonal Lovo lysate 0.5 mg/ml Size 1:500 34
17 beta-HSD1/HSD17B1 Primary R&D Systems
MAB7178
17 beta-HSD1/HSD17B1 Datasheet Mouse Monoclonal Human placenta tissue Size 2.5 µg/mL 40
4-Hydroxynonenal Primary R&D Systems
MAB3249
4-Hydroxynonenal Datasheet Mouse Monoclonal HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 4-Hydroxynonenal (4-HNE) Size 5 µg/mL
4E-BP1, phospho Primary Cell Signaling Technology
9455
Phospho 4E-BP1 (T70) Rabbit polyclonal Human Liver Tissue Charge
4EBP1 Primary R&D Systems
AF3227
4EBP1 Datasheet Goat Polyclonal HeLa human cervical epithelial carcinoma cell line Size 5 µg/mL 26
4EBP1 Primary MilliporeSigma
07-1416
4EBP1 Rabbit polyclonal EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec Charge 1:50-1:200
4EBP1 Primary Epitomics
1557-1
4EBP1 Rabbit monoclonal EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec Charge 1:50-1:200
4EBP1 Primary Cell Signaling Technology
4923
Non-p4EBP1 (Thr46) Rabbit monoclonal EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec Charge 1:50-1:200
4EBP1 Primary Cell Signaling Technology
9452
4EBP1 Rabbit polyclonal Caco2 cell lysates Size 1:50
4EBP1 Primary Cell Signaling Technology
9644
4EBP1 Rabbit monoclonal MCF7 lysed in Bicine/CHAPS Size 1:50-1:100 28-32
4EBP1 Primary Cell Signaling Technology
9644
4EBP1 Rabbit polyclonal EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec Charge 1:200-1:1000
4EBP1, phospho Primary Epitomics
2334-1
p4EBP1 (Thr45) Rabbit monoclonal EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec Charge 1:50-1:200
4EBP1, phospho Primary Cell Signaling Technology
2855
p4EBP1 (Thr37/46) Rabbit monoclonal EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec Charge 1:50-1:200
4EBP1, phospho Primary Cell Signaling Technology
9451
p4EBP1 (Ser65) Rabbit polyclonal K562 -/+ Gleevec lysed in Bicine CHAPS Size 1:50 27-31
4EBP1, phospho Primary Cell Signaling Technology
9451
p4EBP1 (Ser65) Rabbit polyclonal EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec Charge 1:50-1:200
4EBP1, phospho Primary Cell Signaling Technology
9456
p4EBP1 (Ser65) Rabbit monoclonal EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec Charge 1:50-1:200
4EBP1, phospho Primary Cell Signaling Technology
9459
p4EBP1(Thr37/46) Rabbit polyclonal K562 -/+ Gleevec lysed in Bicine CHAPS Size 1:50 27-31
4EBP1, phospho Primary Cell Signaling Technology
9459
p4EBP1 (Thr37/46) Rabbit polyclonal MCF7 lysed in Bicine/CHAPS Charge 1:50-1:100

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