Simple Western™ systems are open platforms, meaning the possibilities are almost endless when selecting antibodies to develop your assays. The Simple Western Antibody database is curated to facilitate the selection process by providing general assay development guidance for identifying and selecting antibodies to test. These guidelines are provided to save time with recommended starting points to optimize for the biological system being examined.
How do antibodies become validated for Simple Western?
- R&D Systems, Novus Biologicals, and valued partners such as Cell Signaling Technology and Bethyl Laboratories are continually validating their antibodies for use with Simple Western.
- Help other scientists and fund your research! Submit new antibody validation data to the Simple Western antibody database and receive a free 8x25 separation module or detection module. Learn more about the Simple Western Antibody Validation Promotion.
- Our expert in-house scientists continue to validate antibodies and review customer-submitted data to ensure the recommendations meet our high standards, so come back for the latest list of validated antibodies in the future.
You can sort by column headings or just type into the Search box. If you don't see your antibody listed here, email us at support@proteinsimple.com.
*Antibodies validated and backed by Cell Signaling Technology (CST)
Simple Western Validated Antibodies
Protein Target | Antibody Type | Vendor / Cat #▲ | Protein Isoform | Citations & Supporting Data | Host Species | Sample | Separation | Dilution | kDa on Simple Western |
---|---|---|---|---|---|---|---|---|---|
β-Tubulin | Primary |
Abcam ab15568 |
Human (β) beta Tubulin aa 400 to the C-terminus | Rabbit Polyclonal | Mouse embryonic fibroblasts | Size | 1:50 | ||
A RAF | Primary |
Abcam ab200653 |
A RAF | Rabbit Monoclonal | A375 cell line | Size | 1:50 | ||
ABCA1 | Primary |
Abcam ab18180 |
ABCA1 | Mouse Monoclonal | Mouse liver lysate | Size | 1:50 | 270 | |
ABCG1 | Primary |
Abcam ab52617 |
ABCG1 | Rabbit monoclonal | Mouse liver lysate | Size | 1:50 | 110 (mouse) -116 (human) | |
ABCG2 | Primary |
Abcam ab24115 |
ABCG2 | Rat monoclonal | 3T3, MCF7, H460 | Size | 1:20 | 100 | |
Actin β | Primary |
Abcam ab8226 |
Cytoskeleton | Mouse Monoclonal | HeLa, Jurkat, HEK 293 | Size | 1:50 | 48 | |
ADAR1 | Primary |
Abcam ab226188 |
ADAR1 | Rabbit Polyclonal | Cell Lysate | Size | 1:25 | 136 | |
Adenosine Receptor A2a | Primary |
Abcam ab3461 |
Adenosine Receptor A2a | Rabbit Polyclonal | Size | 1:50 | 39 | ||
ADH5 | Primary |
Abcam ab59134 |
ADH5 | Goat Polyclonal | Human uterine tissue | Size | 1:100 | ||
AKT1/2/3 | Primary |
Abcam ab32505 |
AKT1/2/3 | Rabbit Monoclonal | Mouse aortas and HUVEC lysate | Size | 1:100 | ||
Alkaline Phosphatase | Primary |
Abcam ab108337 |
Alkaline Phosphatase | Rabbit Monoclonal | ADSCs, FBs, BMSCs | Size | 1:50 | 57 | |
Androgen Receptor | Primary |
Abcam ab198394 |
AR-V7 | Rabbit monoclonal | Human Plasma | Size | 1:50 | 97 | |
Androgen Receptor | Primary |
Abcam ab52615 |
Androgen Receptor | Rabbit Monoclonal | Size | 120 | |||
Angiopoietin 2/ANG2 | Primary |
Abcam ab155106 |
Angiopoietin 2/ANG2 | Rabbit Monoclonal | Untreated A549 Lysate 0.2 mg/mL, A549 Lysate Treated with 1% O2 0.2 mg/mL, A549 Lysate Treated with CoCL2 (4h) 0.2 mg/mL | Size | 1:50 | 39/56/95/145 | |
APC | Primary |
Abcam ab154906 |
APC | Rabbit polyclonal | Hela,K562 | Size | 1:50 | ||
APP | Primary |
Abcam ab32136 |
Amyloid Precursor Protein | Rabbit Monoclonal | Size | 1:200 | 129, 170 | ||
ATM, Phospho | Primary |
Abcam ab81292 |
ATM, Ser1981 | Rabbit Monoclonal | Transfected HeLa, Human Primary Fibroblast Lysates | Size | 1:100 | ||
ATP Synthase | Primary |
Abcam ab96655 |
ATP Synthase | Rabbit Polyclonal | Human bladder tumor cells (T24 cells) and human skin fibroblasts (CAFs) | Size | |||
ATPB | Primary |
Abcam ab14730 |
ATPB | Mouse monoclonal | Mouse brain endothelioma cell line (bEnd5) | Size | 1:500 | ||
Aurora B | Primary |
Abcam ab45145 |
Aurora B | Rabbit Monoclonal | TK6 cells | Size | 1:50 | ||
B Raf | Primary |
Abcam ab33899 |
B Raf | Rabbit Monoclonal | A375 cell line | Size | 1:50 | ||
BCKDHA | Primary |
Abcam ab138460 |
BC | Rabbit polyclonal | Bone Tissue Extracts | Size | 1:50 | 50 | |
Bcl-XL | Primary |
Abcam ab32370 |
Bcl-XL | Rabbit monoclonal | Size | 1:50 | 34 | ||
BCRP/ABCG2 | Primary |
Abcam ab207732 |
Rabbit Monoclonal | Human K-562,K562/A02 | Size | Not_Stated | 72 | ||
BDNF | Primary |
Abcam ab10505 |
BDNF | Mouse monoclonal | Human Saliva | Charge | 1:50 | ||
BDNF | Primary |
Abcam ab10505 |
BDNF | Mouse monoclonal | Human Plasma | Size | 1:50 | ||
BDNF | Primary |
Abcam ab108319 |
Rabbit Monoclonal | Rat Retina | Size | Not_Stated | 15 | ||
Beta actin | Primary |
Abcam Ab6276 |
Beta actin |
Citations
(6) Datasheet |
Mouse monoclonal | Size | 1:25-1:100 | ||
Beta actin | Primary |
Abcam Ab6276 |
Beta actin |
Citations
(6) Datasheet |
Mouse monoclonal | Skin, Kidney, Lung, Instestine | Size | Not_Stated | |
Beta actin | Primary |
Abcam Ab6276 |
Beta actin |
Citations
(6) Datasheet |
Mouse monoclonal | Skin | Size | Not_Stated | |
Beta actin | Primary |
Abcam Ab6276 |
Beta actin |
Citations
(6) Datasheet |
Mouse monoclonal | ARPE-19 | Size | 1:50 | |
Beta actin | Primary |
Abcam Ab6276 |
Beta actin |
Citations
(6) Datasheet |
Mouse monoclonal | Not Stated | Size | Not_Stated | |
Beta actin | Primary |
Abcam Ab6276 |
Beta actin |
Citations
(6) Datasheet |
Mouse monoclonal | T98G | Size | Not_Stated | 66 |
Beta actin | Primary |
Abcam Ab6276 |
Beta actin |
Citations
(6) Datasheet |
Mouse monoclonal | THP-1 | Size | 1:1000 | |
Beta actin | Primary |
Abcam Ab6276 |
Beta actin |
Citations
(6) Datasheet |
Mouse monoclonal | HEK293T | Size | Not_Stated | |
Beta Actin | Primary |
Abcam ab8224 |
Beta actin | Mouse Monoclonal | Primate Corpus Luteum | Size | 1:100 | 49 | |
Beta Actin | Primary |
Abcam ab8224 |
Unmodified |
Citations
(1) Datasheet |
Mouse Monoclonal | Brain | Size | 1:150 | 49.7 |
beta Actin | Primary |
Abcam ab8227 |
beta Actin | Rabbit, polyclonal | mouse oocytes | Size | 1:50 | 47 | |
beta Catenin | Primary |
Abcam ab16051 |
Rabbit Polyclonal | Mouse HEI-OC1 | Size | 1:4000 | 92 | ||
Beta-2 Microglobulin | Primary |
Abcam ab75853 |
Beta-2 Microglobulin | Rabbit monoclonal | Many | Charge | 1:50 | ||
BLK, Phospho | Primary |
Abcam ab192670 |
Phospho Tyr501 | Rabbit Polyclonal | Mouse splenocytes +/- LCWE treatment | Size | |||
BRCA1, Phospho | Primary |
Abcam ab90528 |
BRCA1, Ser1423 | Rabbit Polyclonal | Transfected HeLa, Human Primary Fibroblast Lysates | Size | 1:100 | ||
Cadherin | Primary |
Abcam Ab6528 |
Pan cadherin | Mouse monoclonal | Rat cortex | Size | 1:1000 | ||
Caspase 3 | Primary |
Abcam ab2302 |
Cleaved Caspase 3 | Rabbit polyclonal | K562 cells -/+ Imatinib | Charge | 1:25-1:100 | ||
Caspase 3 | Primary |
Abcam ab32042 |
Cleaved Caspase 3 | Rabbit monoclonal | K562 cells -/+ Imatinib | Charge | 1:50 | ||
CD31 | Primary |
Abcam ab119339 |
Mouse Monoclonal | Human Brain | Size | Not_Stated | 231 | ||
CD4 | Primary |
Abcam ab133622 |
CD4 | Rabbit Monoclonal | Treg cell lysate 0.04 mg/mL, PBMC lysate 0.04 mg/mL | Size | 1:250 | 64 | |
CD63 | Primary |
Abcam ab68418 |
CD63 | Rabbit Polyclonal | Human A549 Cells (Novus Biologicals PN NBP2-49862) PNGase F Treated 0.2 mg/mL | Size | 1:10 | 28 | |
CD83 | Primary |
Abcam ab170855 |
CD83 | Rabbit Monoclonal | Human monocyte-derived dentritic cells 0.5 mg/mL | Size | 1:10 | 44/52/56/77/137 | |
CDKN2A/p16INK4a | Primary |
Abcam ab108349 |
Rabbit Monoclonal | Mouse HEI-OC1 | Size | 1:1000 | 16 |