Sign In / Register
Simple Western™ systems are open platforms, meaning the possibilities are almost endless when selecting antibodies to develop your assays. The Simple Western Antibody database is curated to facilitate the selection process by providing general assay development guidance for identifying and selecting antibodies to test. These guidelines are provided to save time with recommended starting points to optimize for the biological system being examined.
How do antibodies become validated for Simple Western?
- R&D Systems, Novus Biologicals, and valued partners such as Cell Signaling Technology and Bethyl Laboratories are continually validating their antibodies for use with Simple Western.
- Help other scientists and fund your research! Submit new antibody validation data to the Simple Western antibody database and receive a free 8x25 separation module or detection module. Learn more about the Simple Western Antibody Validation Promotion.
- Our expert in-house scientists continue to validate antibodies and review customer-submitted data to ensure the recommendations meet our high standards, so come back for the latest list of validated antibodies in the future.
You can sort by column headings or just type into the Search box. If you don't see your antibody listed here, email us at support@proteinsimple.com.
*Antibodies validated and backed by Cell Signaling Technology (CST)
Simple Western Validated Antibodies
Filter Antibodies: (3371)
| Protein Target | Antibody Type | Vendor / Cat # | Protein Isoform▲ | Citations & Supporting Data | Host Species | Sample | Separation | Dilution | kDa on Simple Western |
|---|---|---|---|---|---|---|---|---|---|
| β-catenin | Primary |
Cell Signaling Technology 8480 |
Datasheet | Rabbit Monoclonal | HL60 Cell Lysates | Size | 1:25, 1:100, 1:400 | 85 | |
| ADAMTS13 | Primary |
R&D Systems AF4245 |
Datasheet | Sheep Polyclonal | Size | 1:50-1:300 | 129, 217 | ||
| AHCY | Primary |
ABclonal A5300 |
Citations
(1) Datasheet |
Rabbit Polyclonal | Size | 1:130 | Not_Stated | ||
| AMP-activated protein kinase (AMPK) | Primary |
MilliporeSigma 07-350 |
Datasheet | Rabbit Polyclonal | L6 cells | Size | 1:10, 1:50, 1:250 | 64 | |
| AMP-activated protein kinase (AMPK) | Primary |
Cell Signaling Technology 2603S |
Datasheet | Mouse Monoclonal | mouse brain tissue | Size | 1:50 | ||
| Anti Sheep-HRP | Secondary |
R&D Systems HAF016 |
Datasheet | Donkey Polyclonal | Size | 1:50 | |||
| Anti-goat-IgG-HRP | Secondary |
Thermo Fisher Scientific 31400 |
Charge | 1:100 | |||||
| Anti-goat-IgG-HRP | Secondary |
Zymax 81-1620 |
Charge | 1:100-1:300 | |||||
| Anti-goat-IgG-HRP | Secondary |
Invitrogen Antibodies 87-9363 |
Size | 1:100-1:300 | |||||
| Anti-Mouse-IgG-HRP | Secondary |
R&D Systems HAF018 |
Datasheet | Donkey Polyclonal | Size | 1:100 | |||
| ATF-3 (D2Y5W) | Primary |
Cell Signaling Technology 33593 |
Citations
(1) Datasheet |
Rabbit Monoclonal | Size | ||||
| BCRP/ABCG2 | Primary |
Abcam ab207732 |
Rabbit Monoclonal | Human K-562,K562/A02 | Size | Not_Stated | 72 | ||
| BDNF | Primary |
Abcam ab108319 |
Rabbit Monoclonal | Rat Retina | Size | Not_Stated | 15 | ||
| beta Catenin | Primary |
Abcam ab16051 |
Rabbit Polyclonal | Mouse HEI-OC1 | Size | 1:4000 | 92 | ||
| Beta-Actin | Primary |
LI-COR 926-42210 |
Rabbit monoclonal | Human neuroblastoma cells | Size | 1:200 | |||
| Beta3-tubulin | Primary |
Santa Cruz Biotechnology sc-80005 |
Datasheet | Mouse Monoclonal | Neuron (RA induced in vitro neural differentiation of NE-4C) | Size | 1:50 | ||
| Bim (C34C5) | Primary |
Cell Signaling Technology 2933 |
Citations
(1) Datasheet |
Mouse Monoclonal | Size | ||||
| Calponin 1 | Primary |
Proteintech 13938-1-AP |
Rabbit polyclonal | human menstrual blood collection | Size | 1:50 | |||
| Catenin-β (Y654), phospho | Primary |
St John's Laboratory STJ90209 |
Rabbit monoclonal | Size | 1:10 | 79 | |||
| CD31 | Primary |
Abcam ab119339 |
Mouse Monoclonal | Human Brain | Size | Not_Stated | 231 | ||
| CDKN2A/p16INK4a | Primary |
Abcam ab108349 |
Rabbit Monoclonal | Mouse HEI-OC1 | Size | 1:1000 | 16 | ||
| Choline acetyltransferase (ChAT) | Primary |
Abcam ab50412 |
Datasheet | Goat Monoclonal | Mouse brain tissue | Size | 1:10, 1:50, 1:250 | 70 | |
| CHOP (L63F7) | Primary |
Cell Signaling Technology 2895 |
Citations
(1) Datasheet |
Mouse Monoclonal | AsPC-1,PK-1 | Size | Not_Stated | ||
| Claudin 5 | Primary |
Abcam ab131259 |
Rabbit Monoclonal | Human Brain | Size | Not_Stated | 28 | ||
| Cyclin D | Primary |
Cell Signaling Technology 2936 |
Datasheet | Mouse Monoclonal | HL60 Cell Lysates | Size | 1:25, 1:100, 1:400 | 38 | |
| EGFR (non-phospho-Tyr1173) | Primary |
MilliporeSigma 05-484 |
Citations
(1) Datasheet |
Mouse Monoclonal | Null | Charge | 0.076388888888888895 | Not_Stated | |
| Fatty Acid Binding Protein 5 | Primary |
Abcam ab128650 |
Rabbit Polyclonal | Mouse Liver | Charge | Not_Stated | 5.5, 5.6 | ||
| FGFR3 |
Cell Signaling Technology 4574 |
Rabbit Monoclonal | Human Spinal Cord | Size | 1:50 | 151 | |||
| Fibrinogen | Primary |
Aglient (DAKO) A0080 |
Rabbit Polyclonal | Citrated mouse plasma stimulated with α-thrombin | Size | 350 | |||
| Flotillin-1 | Primary |
Abcam ab133497 |
Rabbit Monoclonal | A549-derrived exosome standards (Novus NBP3-11645) | Size | 1:250 | 53 | ||
| gamma H2A.X (phospho S139) | Primary |
Abcam ab26350 |
Mouse Monoclonal | Mouse HEI-OC1 | Size | 1:1000 | 15 | ||
| Gankyrin | Primary |
Abcam ab182576 |
Rabbit Monoclonal | Human Bone marrow | Size | 1:50 | 29 | ||
| GATA2 | Primary |
Abcam ab109241 |
Rabbit monoclonal | CD34+ cells from human bone marrow | Size | ||||
| GFP | Primary |
Affinity Biologicals T0005 |
Mouse Monoclonal | Transfected HEK293T & HeLa | Size | 1:50 | 25 | ||
| Glucose transporter 2 (GLUT2) | Primary |
Abcam ab192599 |
Datasheet | Rabbit Monoclonal | Caco-2 cell | Size | 1:10, 1:50, 1:250 | 57-60 | |
| Glycine Receptor alpha 1 + alpha 2 | Primary |
Abcam ab23809 |
Rabbit Polyclonal | Rat Retina | Size | 1:100 | 52 | ||
| GNMT | Primary |
ABclonal A6608 |
Citations
(1) Datasheet |
Rabbit Polyclonal | Liver | Size | 1:130 | Not_Stated | |
| Goat Anti-Rabbit HRP | Secondary |
Molecular Probes A27036 |
Goat oligoclonal | human menstrual blood collection | Size | 1:50 | |||
| GSK3 beta | Primary |
Abcam ab69739 |
Rabbit Polyclonal | Mouse Liver | Charge | Not_Stated | 6.2, 8.8, 8.9, 9.3 | ||
| HIF1a | Primary |
Abcam ab179483 |
Rabbit Monoclonal | Hepatocytes | Size | 1:50 | 110 | ||
| HIF2a | Primary |
Abcam ab199 |
Rabbit Polyclonal | Hepatocytes | Size | 1:50 | 110 | ||
| HSP70 | Primary |
Cell Signaling Technology 4872 |
Rabbit polyclonal | 3T3-L1 | Size | 1:100 | |||
| human RPS19 | Primary |
Bethyl Laboratories A304-002A |
Datasheet | Rabbit Polyclonal | Hudep-2 cell line | Size | 1:100 | 24 | |
| ICAM1 | Primary |
Proteintech 10831-1-AP |
Datasheet | Rabbit Polyclonal | cell | Size | 1:20, 1:100, 1:500 | 95 | |
| JUN | Primary |
Cell Signaling Technology 9165 |
Rabbit Monoclonal | Mouse Retinal Ganglion Cells | Charge | ||||
| MAP3K12 | Primary |
Novus Biologicals NBP2-17218 |
Datasheet | Rabbit Polyclonal | Mouse Retinal Ganglion Cells | Size | |||
| MAT1A | Primary |
ABclonal A6650 |
Citations
(1) Datasheet |
Rabbit Polyclonal | Liver | Size | 1:200 | Not_Stated | |
| MDM4 | Primary |
Bethyl Laboratories A700-000 |
Datasheet | Rabbit Monoclonal | A375 cell line | Size | 1:10, 1:50, 1:100 | 73-81 | |
| Metabotropic Glutamate Receptor 2+3 | Primary |
Abcam ab6438 |
Rabbit Polyclonal | Rat Retina | Size | Not_Stated | 100, 230 | ||
| MRP-1 | Primary |
Abcam ab24108 |
Mouse monoclonal | Tumor-forming endothelial (EOMA) cells | Size | 1:50 |
