Simple Western Antibody Database

Find Antibodies Validated for Simple Western

Simple Western™ systems are open platforms, meaning the possibilities are almost endless when selecting antibodies to develop your assays. The Simple Western Antibody database is curated to facilitate the selection process by providing general assay development guidance for identifying and selecting antibodies to test. These guidelines are provided to save time with recommended starting points to optimize for the biological system being examined.

How do antibodies become validated for Simple Western?

  • R&D Systems, Novus Biologicals, and valued partners such as Cell Signaling Technology and Bethyl Laboratories are continually validating their antibodies for use with Simple Western.
  • Help other scientists and fund your research! Submit new antibody validation data to the Simple Western antibody database and receive a free 8x25 separation module or detection module. Learn more about the Simple Western Antibody Validation Promotion.
  • Our expert in-house scientists continue to validate antibodies and review customer-submitted data to ensure the recommendations meet our high standards, so come back for the latest list of validated antibodies in the future.

You can sort by column headings or just type into the Search box. If you don't see your antibody listed here, email us at support@proteinsimple.com.

*Antibodies validated and backed by Cell Signaling Technology (CST)

Simple Western Validated Antibodies

Filter Antibodies: (3371)
Protein Target Antibody Type Vendor / Cat # Protein Isoform Citations & Supporting Data Host Species Sample▲ Separation Dilution kDa on Simple Western
α-Synuclein Primary Cell Signaling Technology
2642 		(α) alpha-Synuclein Rabbit Polyclonal Size 1:25 21
6X-His tag-HRP Primary Thermo Fisher Scientific
MA1-21315-HRP 		Tag Mouse Monoclonal Size 1:50, 1:100
A. aeolicus BPL/BioID2 Primary Novus Biologicals
NBP2-59940 		A. aeolicus BPL/BioID2 Antibody (SS QD1) Datasheet Mouse Monoclonal Size 94
ABCA1 Primary Novus Biologicals
NB400-105 		ABCA1 Datasheet Rabbit polyclonal Charge 1:50
ACSF2 Primary Novus Biologicals
NBP2-47558 		ACSF2 Antibody Datasheet Rabbit Polyclonal Size
Actinin, alpha Primary Cell Signaling Technology
6487 		Actinin, alpha Rabbit monoclonal Size 1:200
ADAM10 Primary Cell Signaling Technology
14194 		ADAM10 Rabbit Polyclonal Size 60 and 100
ADAMTS13 Primary R&D Systems
AF4245 		Datasheet Sheep Polyclonal Size 1:50-1:300 129, 217
Adenosine Receptor A2a Primary Abcam
ab3461 		Adenosine Receptor A2a Rabbit Polyclonal Size 1:50 39
AHCY Primary ABclonal
A5300 		Citations (1)
Datasheet 		Rabbit Polyclonal Size 1:130 Not_Stated
Akt Primary Cell Signaling Technology
9272 		Unmodified Citations (20)
Datasheet 		Rabbit Polyclonal Size
AKT1 Primary MilliporeSigma
05-796 		AKT1 Rabbit monoclonal Size 1:50
AKT3 Primary MilliporeSigma
07-383 		AKT3 Rabbit polyclonal Size 1:50
alcohol dehydrogenase 5 Primary Novus Biologicals
NBP2-49350 		alcohol dehydrogenase 5 Antibody Datasheet Rabbit Polyclonal Size
Alpha Synuclein Primary BD Biosciences
610787 		Alpha Synuclein Mouse monoclonal Size 1:10 12,20
alpha-Fetoprotein/AFP Primary Novus Biologicals
NB110-7961 		alpha-Fetoprotein/AFP Datasheet Mouse Monoclonal Size
AMPK alpha, phospho Primary Cell Signaling Technology
2531 		AMPKα, Phospho (Thr172) Rabbit Polyclonal Size 63
Androgen Receptor Primary Abcam
ab52615 		Androgen Receptor Rabbit Monoclonal Size 120
Anti Sheep-HRP Secondary R&D Systems
HAF016 		Datasheet Donkey Polyclonal Size 1:50
Anti-bird-IgG-HRP Secondary Bethyl Laboratories
A140-110P 		Anti-bird-IgG-HRP Size 1:250
Anti-Chicken IgY (IgG) (H+L) HRP Secondary Jackson ImmunoResearch
103-035-155 		Anti-Chicken IgY (IgG) (H+L) HRP Conjugated Minimal Cross Reactivity Goat Polyclonal Size 1:1000
Anti-chicken-IgG-HRP Secondary KPL
14-24-06 		Anti-chicken-IgG-HRP Size 1:300
Anti-Goat IgG (H+L) Biotin-SP Secondary Jackson ImmunoResearch
305-065-045 		Anti-Goat IgG (H+L) Biotin-SP Conjugated Minimal Cross Reactivity Goat Polyclonal Charge 1:1000
Anti-Goat IgG (H+L) HRP Secondary Jackson ImmunoResearch
705-035-003 		Anti-Goat IgG (H+L) HRP Conjugated Donkey Polyclonal Charge 1:250
Anti-Goat IgG (H+L) HRP Secondary Jackson ImmunoResearch
705-035-147 		Anti-Goat IgG (H+L) HRP Conjugated Minimal Cross Reactivity Donkey Polyclonal Charge 1:100-1:300
Anti-Goat IgG (H+L) HRP Secondary Jackson ImmunoResearch
705-035-147 		Anti-Goat IgG (H+L) HRP Conjugated Minimal Cross Reactivity Donkey Polyclonal Size 1:100-1:300
Anti-Goat IgG (H+L) HRP Secondary Jackson ImmunoResearch
805-035-180 		Anti-Goat IgG (H+L) HRP Conjugated Bovine Polyclonal Charge
Anti-goat-IgG-HRP Secondary Thermo Fisher Scientific
31400 		Charge 1:100
Anti-goat-IgG-HRP Secondary Zymax
81-1620 		Charge 1:100-1:300
Anti-goat-IgG-HRP Secondary Invitrogen Antibodies
87-9363 		Size 1:100-1:300
Anti-Goat/Sheep IgG HRP Secondary MilliporeSigma
A9452 		Anti-Bovine/Sheep/Goat IgG HRP Conjugated Mouse Monoclonal Size 1:100
Anti-Human IgG (H+L) Biotin-SP Secondary Jackson ImmunoResearch
109-065-003 		Anti-Human IgG (H+L) Biotin-SP Conjugated Goat Polyclonal Charge 1:1000
Anti-Human IgG (H+L) HRP Secondary Jackson ImmunoResearch
109-035-003 		Anti-Human IgG (H+L) HRP Conjugated Goat Polyclonal Charge 1:100-1:300
Anti-Human IgG (H+L) HRP Secondary Jackson ImmunoResearch
109-035-003 		Anti-Human IgG (H+L) HRP Conjugated Goat Polyclonal Size 1:100-1:300
Anti-Human IgG Fc Secondary Thermo Fisher Scientific
31142 		Human IgG Fc Rabbit Polyclonal Size 1:400
Anti-Human IgG Fc Secondary Thermo Fisher Scientific
MA1-81864 		Human IgG Fc (CH2 domain) Mouse Monoclonal Size 1:400
Anti-Mouse IgG (H+L) HRP Secondary Jackson ImmunoResearch
115-035-062 		Anti-Mouse IgG (H+L) HRP Conjugated Minimal Cross Reactivity Goat Polyclonal Size 1:100
Anti-Mouse-IgG-HRP Secondary R&D Systems
HAF018 		Datasheet Donkey Polyclonal Size 1:100
Anti-Rabbit IgG (H+L) HRP Secondary Novus Biologicals
NB7185 		Rabbit IgG (H+L) Pre-adsorbed, HRP Conjugated Datasheet Donkey Polyclonal Size 1:50
Anti-Rabbit IgG (H+L) HRP Secondary MilliporeSigma
A0545 		Anti-Rabbit IgG (H+L) HRP Conjugated Minimal Cross Reactivity to Human Goat Polyclonal Charge
Anti-Rabbit IgG (H+L) Poly-HRP Secondary Thermo Fisher Scientific
32260 		Anti-Rabbit IgG (H+L) Poly-HRP Goat Polyclonal Size 1:100
Anti-Rat IgG (H+L) HRP Secondary Jackson ImmunoResearch
712-035-153 		Anti-Rat IgG (H+L) HRP Conjugated Donkey Polyclonal Size 1:100-1:300
Anti-Sheep IgG (H+L) HRP Secondary Jackson ImmunoResearch
713-036-147 		F(ab')2 Fragment Donkey Anti-Sheep IgG (H+L) HRP Conjugated Minimal Cross Reactivity Donkey Polyclonal Size 1:100
Anti-Sheep IgG (H+L) HRP Secondary MilliporeSigma
A3415 		Anti-Sheep IgG (H+L) HRP Conjugated Donkey Polyclonal Size 1:100
APP Primary Abcam
ab32136 		Amyloid Precursor Protein Rabbit Monoclonal Size 1:200 129, 170
ATF-3 (D2Y5W) Primary Cell Signaling Technology
33593 		Citations (1)
Datasheet 		Rabbit Monoclonal Size
ATR, phospho Primary Cell Signaling Technology
2853 		ATR, Phospho (Ser428) Rabbit Polyclonal Size 300
Bcl-XL Primary Abcam
ab32370 		Bcl-XL Rabbit monoclonal Size 1:50 34
BDNF Primary Novus Biologicals
NB120-10505 		BDNF Antibody (35928.11) Datasheet Mouse Monoclonal Size
Beta actin Primary Cell Signaling Technology
4967 		b-actin (may cross react with g-actin) Rabbit polyclonal Size 1:50 45
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