Simple Western Antibody Database

Find Antibodies Validated for Simple Western

Simple Western™ systems are open platforms, meaning the possibilities are almost endless when selecting antibodies to develop your assays. The Simple Western Antibody database is curated to facilitate the selection process by providing general assay development guidance for identifying and selecting antibodies to test. These guidelines are provided to save time with recommended starting points to optimize for the biological system being examined.

How do antibodies become validated for Simple Western?

  • R&D Systems, Novus Biologicals, and valued partners such as Cell Signaling Technology and Bethyl Laboratories are continually validating their antibodies for use with Simple Western.
  • Help other scientists and fund your research! Submit new antibody validation data to the Simple Western antibody database and receive a free 8x25 separation module or detection module. Learn more about the Simple Western Antibody Validation Promotion.
  • Our expert in-house scientists continue to validate antibodies and review customer-submitted data to ensure the recommendations meet our high standards, so come back for the latest list of validated antibodies in the future.

You can sort by column headings or just type into the Search box. If you don't see your antibody listed here, email us at support@proteinsimple.com.

*Antibodies validated and backed by Cell Signaling Technology (CST)

Simple Western Validated Antibodies

Filter Antibodies: (3371)
Protein Target Antibody Type Vendor / Cat # Protein Isoform Citations & Supporting Data Host Species Sample Separation Dilution kDa on Simple Western▲
α-Tubulin Primary MilliporeSigma
T8203 		alpha Tubulin Mouse Monoclonal Transfected HeLa, Human Primary Fibroblast Lysates Size 1:100
β-Actin Primary MilliporeSigma
A5316 		beta-actin Mouse Monoclonal Transfected HeLa, Human Primary Fibroblast Lysates Size 1:100
β-Catenin Primary Cell Signaling Technology
9562 		(β) beta-Catenin Rabbit Polyclonal HeLa Size 1:50
β-Tubulin Primary Abcam
ab15568 		Human (β) beta Tubulin aa 400 to the C-terminus Rabbit Polyclonal Mouse embryonic fibroblasts Size 1:50
β-Tubulin Primary MilliporeSigma
T4026 		(β) beta-Tubulin Mouse Monoclonal Huh7.5.1 Size
4-Hydroxynonenal Primary R&D Systems
MAB3249 		4-Hydroxynonenal Datasheet Mouse Monoclonal HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 4-Hydroxynonenal (4-HNE) Size 5 µg/mL
4E-BP1, phospho Primary Cell Signaling Technology
9455 		Phospho 4E-BP1 (T70) Rabbit polyclonal Human Liver Tissue Charge
4EBP1 Primary MilliporeSigma
07-1416 		4EBP1 Rabbit polyclonal EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec Charge 1:50-1:200
4EBP1 Primary Epitomics
1557-1 		4EBP1 Rabbit monoclonal EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec Charge 1:50-1:200
4EBP1 Primary Cell Signaling Technology
4923 		Non-p4EBP1 (Thr46) Rabbit monoclonal EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec Charge 1:50-1:200
4EBP1 Primary Cell Signaling Technology
9452 		4EBP1 Rabbit polyclonal Caco2 cell lysates Size 1:50
4EBP1 Primary Cell Signaling Technology
9644 		4EBP1 Rabbit polyclonal EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec Charge 1:200-1:1000
4EBP1, phospho Primary Epitomics
2334-1 		p4EBP1 (Thr45) Rabbit monoclonal EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec Charge 1:50-1:200
4EBP1, phospho Primary Cell Signaling Technology
2855 		p4EBP1 (Thr37/46) Rabbit monoclonal EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec Charge 1:50-1:200
4EBP1, phospho Primary Cell Signaling Technology
9451 		p4EBP1 (Ser65) Rabbit polyclonal EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec Charge 1:50-1:200
4EBP1, phospho Primary Cell Signaling Technology
9456 		p4EBP1 (Ser65) Rabbit monoclonal EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec Charge 1:50-1:200
4EBP1, phospho Primary Cell Signaling Technology
9459 		p4EBP1 (Thr37/46) Rabbit polyclonal MCF7 lysed in Bicine/CHAPS Charge 1:50-1:100
4EBP1, phospho Primary Cell Signaling Technology
9459 		p4EBP1 (Thr37/46) Rabbit polyclonal EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec Charge 1:50-1:200
4EBP2 Primary Cell Signaling Technology
2845 		4EBP2 Rabbit polyclonal EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562-/+Gleevec Charge 1:50-1:100
5-HT2AR Primary LSBio
LS-C172270 		5-HT2AR Rabbit polyclonal Rat cortex Size 1:500
5-Lipoxygenase Primary Novus Biologicals
NB110-58748 		5-Lipoxygenase Datasheet Rabbit polyclonal Human Lung lysate 0.5 mg/ml Size 50 µg/mL
5-Lipoxygenase Primary Novus Biologicals
NB110-58749 		5-Lipoxygenase Datasheet Rabbit polyclonal Human Lung lysate 0.5 mg/ml Size 10 µg/mL
5HT1D Primary Novus Biologicals
NB100-56349 		5HT1D Datasheet Rabbit polyclonal Human Brain lysate 0.5 mg/ml Size 1:10
6-His Epitope Tag Primary Novus Biologicals
NB100-64768 		6-His Epitope Tag Datasheet Mouse monoclonal rhEDG6 CHO S Tx lysate 0.1 mg/ml Size 1:250
6X-His tag-HRP Primary Thermo Fisher Scientific
MA1-21315-HRP 		Tag Mouse Monoclonal Size 1:50, 1:100
A RAF Primary Abcam
ab200653 		A RAF Rabbit Monoclonal A375 cell line Size 1:50
A20 Primary Origene
TA318419 		A20 Rabbit Caco cell lysate Size
A20 (TNFa-induced protein 1 member 3) Primary Origene
TA307167 		A20 Rabbit monoclonal Caco2 cell lysates Size 1:50
ABCA1 Primary Novus Biologicals
NB400-105 		ABCA1 Datasheet Rabbit polyclonal Charge 1:50
Acetyl-CoA carboxylase Primary Cell Signaling Technology
3661 		Ser79 Rabbit Polyclonal HEK293T Size 1:200
Acetyl-CoA Carboxylase (ACC), phospho Primary Cell Signaling Technology
3661 		pACC (Ser 79) Rabbit Polyclonal HEK 293 Size 1:200
Acetylated Lysine Primary Cell Signaling Technology
9441 		Acetylated-Lysine Rabbit Polyclonal Huh7.5 Charge 1:50
ACSF2 Primary Novus Biologicals
NBP2-47558 		ACSF2 Antibody Datasheet Rabbit Polyclonal Size
ACSL3 Primary Novus Biologicals
NBP2-15252 		ACSL3 Antibody Datasheet Rabbit Polyclonal Mouse brain (hippocampus) tissue Size
ACSL5 Primary Novus Biologicals
NBP1-59645 		ACSL5 Antibody Datasheet Rabbit Polyclonal Mouse liver lysate Size
Actin Primary Sigma-Aldrich
A3853 		Actin Mouse monoclonal Viral particles in chicken eggs Size 1:25
Actin (C-2) Primary Santa Cruz Biotechnology
sc-8432 		Actin Mouse Monoclonal Mouse brain lysate Size 1:50
Actinin, alpha Primary Cell Signaling Technology
6487 		Actinin, alpha Rabbit monoclonal Size 1:200
ADH5 Primary Abcam
ab59134 		ADH5 Goat Polyclonal Human uterine tissue Size 1:100
AIF Primary Novus Biologicals
NBP2-27177 		AIF Datasheet Rabbit polyclonal Jurkat and MCF-7 lysate 0.2 mg/ml Size 1:500
AKR1D1 (5βR1) Primary Thermo Fisher Scientific
PA5-28963 		5βR1 Rabbit Polyclonal Human fetal adrenal glands Size 1:100
AKT Primary Cell Signaling Technology
2920 		AKT1/2/3 Mouse monoclonal MEF and NHLF Charge 1:100
AKT Primary Cell Signaling Technology
2920 		Total AKT Mouse Monoclonal SH-SY5Y Size 1:50
AKT Primary Cell Signaling Technology
4691 		AKT Citations (5)
Datasheet 		Rabbit monoclonal MCF7 lysed in Bicine/CHAPS Charge 1:50-1:100
AKT Primary Cell Signaling Technology
9272 		AKT1/2/3 Rabbit polyclonal EGF stimulated MCF-7 Charge 1:50
Akt Primary Cell Signaling Technology
9272 		Unmodified Citations (20)
Datasheet 		Rabbit Polyclonal Size
Akt Primary Cell Signaling Technology
9272 		Unmodified Citations (20)
Datasheet 		Rabbit Polyclonal N1E-115, Brain Size Not_Stated
AKT, phospho Primary Cell Signaling Technology
13038 		Phospho AKT (T308) Rabbit monoclonal Human Liver Tissue Charge
AKT, phospho Primary Epitomics
2118-1 		pAKT (Ser473) Rabbit monoclonal LNCaP Size 1:50
AKT, phospho Primary Epitomics
2118 		pAKT (Ser473) Rabbit monoclonal EGF stimulated MCF-7 Charge 1:50
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